LEADER 04088nam 2200649 a 450 001 9910130871003321 005 20200520144314.0 010 $a1-283-23936-1 010 $a9786613239365 010 $a0-470-66982-9 010 $a0-470-66981-0 035 $a(CKB)3460000000000123 035 $a(EBL)675259 035 $a(OCoLC)742333230 035 $a(SSID)ssj0000482393 035 $a(PQKBManifestationID)12160432 035 $a(PQKBTitleCode)TC0000482393 035 $a(PQKBWorkID)10524765 035 $a(PQKB)10726279 035 $a(MiAaPQ)EBC675259 035 $a(Au-PeEL)EBL675259 035 $a(CaPaEBR)ebr10492727 035 $a(CaONFJC)MIL323936 035 $a(PPN)172033675 035 $a(EXLCZ)993460000000000123 100 $a20100910d2011 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 00$aAnimal cell culture$b[electronic resource] $eessential methods /$feditor, John M. Davis 210 $aHoboken, N.J. $cJohn Wiley & Sons Inc.$d2011 215 $a1 online resource (380 p.) 225 1 $aEssential Methods 300 $aDescription based upon print version of record. 311 $a0-470-97563-6 311 $a0-470-66658-7 320 $aIncludes bibliographical references and index. 327 $aCONTENTS; Contributors; Preface; Abbreviations; Protocols; 1 The Cell Culture Laboratory; 1.1 Introduction; 1.2 Methods and approaches; 1.3 Troubleshooting; Acknowledgements; References; 2 Sterilization; 2.1 Introduction; 2.2 Methods and approaches; 2.3 Troubleshooting; References; 3 Microscopy of Living Cells; 3.1 Introduction; 3.2 Methods and approaches; 3.3 Troubleshooting; Acknowledgements; References; 4 Basic Techniques and Media, the Maintenance of Cell Lines,and Safety; 4.1 Introduction; 4.2 Methods and approaches; 4.3 Troubleshooting; Acknowledgements; References 327 $a5 Development and Optimization of Serum- and Protein-freeCulture Media5.1 Introduction; 5.2 Methods and approaches; 5.3 Troubleshooting; 5.4 Conclusion; Acknowledgements; References; 6 Cryopreservation and Banking of Cell Lines; 6.1 Introduction; 6.2 Methods and approaches; 6.3 Troubleshooting; Acknowledgements; References; 7 Primary Culture of Specific Cell Types and the Establishmentof Cell Lines; 7.1 Introduction; 7.2 Methods and approaches; 7.3 Troubleshooting; References; 8 Cloning; 8.1 Introduction; 8.2 Methods and approaches; 8.3 Troubleshooting; References 327 $a9 The Quality Control of Animal Cell Lines and the Prevention,Detection and Cure of Contamination9.1 Introduction; 9.2 Methods and approaches; 9.3 Troubleshooting; References; 10 Systems for Cell Culture Scale-up; 10.1 Introduction; 10.2 Methods and approaches; 10.3 Troubleshooting; References; 11 Good Laboratory Practice in the Cell Culture Laboratory; 11.1 Introduction; 11.2 Background to GLP; 11.3 General GLP principles; 11.4 Study performance; 11.5 Good Manufacturing Practice; 11.6 Summary; References; Index 330 $aThis is a comprehensive research guide that describes both the key new techniques and more established methods. Every chapter discusses the merits and limitations of the various approaches and then provides selected tried-and-tested protocols, as well as a plethora of good practical advice, for immediate use at the bench. It presents the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells. Detailed protocols for a wide variety of methods provide the core of each chapter, making new methodology easily accessible.This book is 410 0$aEssential Methods 606 $aCell culture$xTechnique 606 $aTissue culture$xTechnique 615 0$aCell culture$xTechnique. 615 0$aTissue culture$xTechnique. 676 $a571.6/381 701 $aDavis$b John$f1952-$0868987 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910130871003321 996 $aAnimal cell culture$92262367 997 $aUNINA