LEADER 03183nam 22006134a 450 001 9910144671803321 005 20230920152815.0 010 $a1-282-25217-8 010 $a9786612252174 010 $a0-470-69888-8 010 $a0-470-69834-9 035 $a(CKB)1000000000687665 035 $a(EBL)454295 035 $a(OCoLC)608624480 035 $a(SSID)ssj0000226016 035 $a(PQKBManifestationID)11220448 035 $a(PQKBTitleCode)TC0000226016 035 $a(PQKBWorkID)10233346 035 $a(PQKB)10057753 035 $a(MiAaPQ)EBC454295 035 $a(Au-PeEL)EBL454295 035 $a(CaPaEBR)ebr10315644 035 $a(CaONFJC)MIL225217 035 $a(EXLCZ)991000000000687665 100 $a20031007d2004 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt$2rdacontent 182 $cc$2rdamedia 183 $acr$2rdacarrier 200 00$aPractice development in nursing /$fedited by Brendan McCormack, Kim Manley, and Robert Garbett 205 $a1st ed. 210 $aOxford ;$aMalden, Mass. $cBlackwell Pub.$d2004 215 $a1 online resource (xvi, 336 pages) $cillustrations 311 0 $a1-4051-1038-4 320 $aIncludes bibliographical references and index. 327 $aA concept analysis of practice development -- Practice development: purpose, methodology, facilitation, & evaluation -- Transformational culture: a culture of effectiveness -- Evaluating practice developments -- Research implementation: evidence, context and facilitation - the PARIHS framework -- Helping relationships for practice development: critical companionship -- Including the older person with a dementia in practice development -- Practice development in child health nursing: a personal perspective -- 327 $aAcute mental healthcare: transforming cultures, a practice development approach -- Developing a corporate strategy to develop effective and patient-centred care -- From conception to delivery: a journey to a trust-wide strategy to develop a culture of patient-centeredness -- Developing and implementing a family health assessment: from project worker to practice developer -- A clearer vision of practice development? 330 $aPractice development depends on understanding current research and practice in order to improve healthcare for patients and users. Practice Development in Nursing explores the basis of practice development, its aims, implementation and impact on health care, and goes on to propose a conceptual basis for developing practice. It is aimed at practitioners, managers, and educators as well as those with a primary practice development role, in order to enable them to effectively develop practice. 606 $aNursing 606 $aNurse practitioners 615 0$aNursing. 615 0$aNurse practitioners. 676 $a610.73 701 $aMcCormack$b Brendan$0853506 701 $aManley$b Kim$cMN.$0863031 701 $aGarbett$b Robert$0863032 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910144671803321 996 $aPractice development in nursing$91926524 997 $aUNINA LEADER 01153nam0 22003133i 450 001 UBO3138069 005 20231121125900.0 010 $a8884924022 010 $a9788884924025 100 $a20160204d2006 ||||0itac50 ba 101 | $aeng 102 $ait 181 1$6z01$ai $bxxxe 182 1$6z01$an 200 1 $aPower and culture$ehegemony, interaction and dissent$fedited by Ausma Cimdina and Jonathan Osmond 210 $aPisa$cPlus$dc2006 215 $aXIII, 177 p.$cill.$d24 cm. 225 | $aThematic work group$i. 2, Power and culture$v1 410 0$1001USM1797245$12001 $aThematic work group$i. 2, Power and culture$v1 702 1$aOsmond$b, Jonathan$3LO1V130064 702 1$aCimdina$b, Ausma$3USMV642711 801 3$aIT$bIT-01$c20160204 850 $aIT-FR0017 899 $aBiblioteca umanistica Giorgio Aprea$bFR0017 912 $aUBO3138069 950 0$aBiblioteca umanistica Giorgio Aprea$d 52CIS 12/962$e 52VM 0000819725 VM barcode:00076442. - Inventario:33794 FLSVM$fA $h20100225$i20121204 977 $a 52 996 $aPower and culture$91118290 997 $aUNICAS LEADER 11105nam 2200529 450 001 9910830392803321 005 20230623153210.0 010 $a1-119-81408-1 010 $a1-119-81406-5 035 $a(MiAaPQ)EBC7219877 035 $a(Au-PeEL)EBL7219877 035 $a(OCoLC)1374426480 035 $a(EXLCZ)9926323395900041 100 $a20230604d2023 uy 0 101 0 $aeng 135 $aurcnu|||||||| 181 $ctxt$2rdacontent 182 $cc$2rdamedia 183 $acr$2rdacarrier 200 00$aMicrobiological identification using MALDI-TOF and tandem mass spectrometry $eindustrial and environmental applications /$fedited by Saheer E. Gharbia [and four others] 210 1$aChichester, England :$cJohn Wiley & Sons Ltd,$d[2023] 210 4$d©2023 215 $a1 recurso en liña (560 páxinas) 311 08$aPrint version: Gharbia, Saheer E. Microbiological Identification Using MALDI-TOF and Tandem Mass Spectrometry Newark : John Wiley & Sons, Incorporated,c2023 9781119814054 320 $aIncludes bibliographical references and index. 327 $aCover -- Title Page -- Copyright Page -- Contents -- List of Contributors -- Preface -- Chapter 1 Progress in the Microbiological Applications of Mass Spectrometry: from Electron Impact to Soft Ionization Techniques, MALDI-TOF MS and Beyond -- 1.1 Introduction -- 1.1.1 Algorithms Based upon Traditional Carbohydrate Fermentation Tests -- 1.1.2 Dynamic Changes in the Chemotaxonomic Era (c. 1970-1985) through the Lens of the Genus Bacteroides -- 1.1.3 Microbial Lipids as Diagnostic Biomarkers -- Resurgence of Interest in MALDI-TOF MS with Advances in Lipidomics -- 1.2 The Dawn of MALDI-TOF MS: Establishing Proof of Concept for Diagnostic Microbiology -- 1.2.1 Development of a MALDI-TOF MS Database for Human Infectious Diseases -- 1.2.2 The Dilemma with Clostridium difficile: from Intact Cells to Intracellular Proteins, MALDI-TOF MS Enters a New Phase -- 1.3 Linear/Reflectron MALDI-TOF MS to Tandem Mass Spectrometry -- 1.3.1 Tandem MALDI-TOF Mass Spectrometry -- 1.3.2 Electrospray-based Mass Analysers -- 1.3.3 Tandem Mass Spectrometry -- 1.3.4 Mass Spectrometry-based Proteomics -- 1.3.5 Case Study: LC-MS/MS of Biothreat Agents, Proteomes of Pathogens and Strain-level Tying Using Bottom-up and Top-down Proteomics -- 1.3.6 Discovery Proteomics -- 1.3.7 Targeted Proteomics -- 1.3.8 Top-down Proteomics -- 1.3.9 Targeted Protein Quantitation -- 1.4 The Application of MALDI-MS Profiling and Imaging in Microbial Forensics: Perspectives -- 1.4.1 MALDI-MSP of Microorganisms and their Products -- 1.5 Hydrogen/Deuterium Exchange Mass Spectrometry in Microbiology -- 1.6 The Omnitrap, a Novel MS Instrument that Combines Many Applications of Mass Spectrometry -- References -- Chapter 2 Machine Learning in Analysis of Complex FloraUsing Mass Spectrometry -- 2.1 Introduction. 327 $a2.2 An Improved MALDI-TOF MS Data Analysis Pipeline for the Identification of Carbapenemase-producing Klebsiella pneumoniae -- 2.2.1 Motivation -- 2.2.2 Materials and Methods -- 2.2.3 Spectra Acquisition -- 2.2.4 Results -- 2.2.5 Discussion -- 2.3 Detection of Vancomycin-Resistant Enterococcus faecium -- 2.3.1 Motivation -- 2.3.2 Materials and Methods -- 2.3.3 Results and Discussion -- 2.4 Detection of Azole Resistance in Aspergillus fumigatus Complex Isolates -- 2.4.1 Introduction -- 2.4.2 Material and Methods -- 2.4.3 Results -- 2.4.4 Discussion -- 2.5 Peak Analysis for Discrimination of Cryptococcus neoformans Species Complex and their Interspecies Hybrids -- 2.5.1 Motivation -- 2.5.2 Material and Methods -- 2.5.3 Results and Discussion -- 2.6 Conclusions -- References -- Chapter 3 Top-down Identification of Shiga Toxin (and Other Virulence Factors and Biomarkers) from Pathogenic E. coli using MALDI-TOF/TOF Tandem Mass Spectrometry -- 3.1 Introduction -- 3.2 Decay of Metastable Peptide and Protein Ions by the Aspartic Acid Effect -- 3.3 Energy Deposition during Desorption/Ionization by MALDI -- 3.4 Protein Denaturation and Fragmentation Efficiency of PSD -- 3.5 Arginine and its Effect on Fragment Ion Detection and MS/MS Spectral Complexity -- 3.6 Inducing Gene Expression in Wild-type Bacteria for Identification by Top-Down Proteomic Analysis -- 3.7 Top-down Proteomic Identification of B-Subunit of Shiga Toxin from STEC Strains -- 3.8 Furin-digested Shiga Toxin and Middle-down Proteomics -- 3.9 Top-down Identification of an Immunity Cognate of a Bactericidal Protein Produced from a STEC Strain -- 3.10 LC-MALDI-TOF/TOF -- 3.11 Conclusions -- References -- Chapter 4 Liquid Atmospheric Pressure (LAP) - MALDI MS(/MS) Biomolecular Profiling for Large-scale Detection of Animal Disease and Food Adulteration and Bacterial Identification -- 4.1 Introduction. 327 $a4.2 Background to LAP-MALDI MS -- 4.3 Bacterial Identification by LAP-MALDI MS -- 4.4 Food Adulteration and Milk Quality Analysis by LAP-MALDI MS -- 4.5 Animal Disease Detection by LAP-MALDI MS -- 4.6 Antibiotic Resistance Detection of Microbial Consortia by LAP-MALDI MS -- 4.7 Future Directions for LAP-MALDI MS Applications -- References -- Chapter 5 Development of a MALDI-TOF Mass Spectrometry Test for Viruses -- 5.1 Introduction -- 5.2 Understanding the Systems Biology of the Virus and Viral Infections -- 5.3 Understanding the Nature of Viral Proteins and Molecular Biology -- 5.4 Virion Protein Solubilization and Extraction -- 5.5 Sampling and Virion Enrichment -- 5.6 Peak Identification: Quantification and Bioinformatics -- 5.7 Promise and Pitfalls of Machine Learning Bioinformatics -- 5.8 Accelerating MALDI-TOF Assay Protocol Development Using Pseudotypes/pseudoviruses -- 5.9 Understanding the Operational Parameters of your MALDI-TOF MS -- 5.10 Understanding the Operational Requirements of the Clinical Testing Laboratory: Validation and International Accreditation -- 5.10.1 Limitation and Advantages of CLIA LDTs -- 5.11 MALDI-TOF MS Screening Test for SARS-CoV-2s -- 5.11.1 Prepare Positive Control -- 5.11.2 Prepare Gargle-saliva Samples -- 5.11.3 Viral Particle Enrichment -- 5.11.4 Dissolution of Virions and Solubilization of Viral Proteins -- 5.11.5 MALDI-TOF MS -- 5.12 CLIA LDT Validation of a MALDI-TOF MS Test for SARS-CoV-2 -- 5.12.1 Limit of Detection -- 5.12.2 Interfering Substances and Specificity -- 5.12.3 Clinical Performance Evaluation -- 5.12.4 Reproducibility -- 5.12.5 Stability -- 5.12.6 Validation Disposition -- References -- Chapter 6 A MALDI-TOF MS Proteotyping Approach for Environmental, Agricultural and Food Microbiology -- 6.1 Introduction -- 6.2 Serotyping of Salmonella enterica Subspecies enterica. 327 $a6.3 Discrimination of the Lineages of Listeria monocytogenes and Species of Listeria -- 6.4 Discrimination of the Bacillus cereus Group and Identification of Cereulide -- 6.5 Identification of Alkylphenol Polyethoxylate-degrading Bacteria in the Environment -- 6.6 Conclusions and Future Perspectives -- References -- Chapter 7 Diversity, Transmission and Selective Pressure on the Proteome of Pseudomonas aeruginosa -- 7.1 Introduction: Diversity -- 7.1.1 P. aeruginosa: from 'Atypical' to Diverse -- 7.1.2 Phenotypical Diversity in Isolates from Different Environments -- 7.1.3 The Relationship Between Phenotypical and Proteomic Diversity -- 7.1.4 Techniques and Practical Considerations for Studying Proteomic Diversity -- 7.1.5 Proteomic Diversity and MS Applications -- 7.2 Transmission -- 7.2.1 The History of P. aeruginosa Transmission -- 7.2.2 Proteomics and P. aeruginosa Transmission -- 7.2.3 The Impact of Proteomic Diversity on Transmission -- 7.3 Selective Pressures on the Proteome -- 7.3.1 Tandem MS Systems for Studying Selected Proteomes -- 7.3.2 Microenvironment Selection -- 7.3.3 Antimicrobial Selection -- 7.4 Conclusions on Studies of the Proteome -- 7.5 Genomic Studies on Pseudomonas aeruginosa Strains Revealing the Presence of Two Distinct Clades -- 7.5.1 Phylogenomic Analysis Reveals the Presence of Two Distinct Clades Within P. aeruginosa -- 7.5.2 Identification of Molecular Markers Distinguishing the Two P. aeruginosa Clades -- 7.6 Final Conclusions -- References -- Chapter 8 Characterization of Biodegradable Polymers by MALDI-TOF MS -- 8.1 Introduction -- 8.2 Structural Characterization of Poly(-caprolactone) Using MALDI-TOF MS -- 8.3 Biodegradation Profiles of a Terminal-modified PCL Observed by MALDI-TOF MS -- 8.4 Bacterial Biodegradation Mechanisms of Non-ionic Surfactants. 327 $a8.5 Advanced Molecular Characterization by High-resolution MALDI-TOF MS Combined with KMD Analysis -- 8.6 Structural Characterization of High-molecular-weight Biocopolyesters by High-resolution MALDI-TOF MS Combined with KMD Analysis -- References -- Chapter 9 Phytoconstituents and Antimicrobiological Activity -- 9.1 Introduction to Phytochemicals -- 9.2 An Application to Bacteriology -- 9.2.1 Allicin Leads to a Breakdown of the Cell Wall of Staphylococcus aureus -- 9.3 Applications to Parasitology -- 9.3.1 Drug Discovery -- 9.3.2 Parasite Characterization -- 9.4 A Proteomic Approach: Leishmania Invasion of Macrophages -- 9.5 Intracellular Leishmania Amastigote Spreading between Macrophages -- 9.6 Potential Virus Applications -- Acknowledgements -- References -- Chapter 10 Application of MALDI-TOF MS in Bioremediation and Environmental Research -- 10.1 Introduction -- 10.2 Microbial Identification: Molecular Methods and MALDI-TOF MS -- 10.2.1 PCR-based Methods -- 10.2.2 MALDI-TOF MS -- 10.3 Combination of MALDI-TOF MS with Other Methods for the Identification of Microorganisms -- 10.4 Application of MALDI-TOF MS in Environmental and Bioremediation Studies -- 10.4.1 The Atmospheric Environment -- 10.4.2 The Aquatic Environment -- 10.4.3 The Terrestrial Environment -- 10.4.4 Bioremediation Research Applications -- 10.5 Microbial Products and Metabolite Activity -- 10.6 Challenges of Environmental Applications -- 10.7 Opportunities and Future Outlook -- 10.8 Conclusions -- References -- Chapter 11 From Genomics to MALDI-TOF MS: Diagnostic Identification and Typing of Bacteria in Veterinary Clinical Laboratories -- 11.1 Introduction -- 11.2 Genomics -- 11.3 Defining Bacterial Species Through Genomics -- 11.4 MALDI-TOF MS -- 11.5 Combining Genomics with MALDI-TOF MS to Classify Bacteria at the Subspecies Level -- 11.6 Data Exploration with MALDI-TOF MS. 327 $a11.7 Validation of Typing Strategies. 606 $aBiomolecules$xAnalysis 606 $aMatrix-assisted laser desorption-ionization 606 $aTime-of-flight mass spectrometry 615 0$aBiomolecules$xAnalysis. 615 0$aMatrix-assisted laser desorption-ionization. 615 0$aTime-of-flight mass spectrometry. 676 $a572.36 702 $aGharbia$b Saheer 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 801 2$bES_ScU 906 $aBOOK 912 $a9910830392803321 996 $aMicrobiological identification using MALDI-TOF and tandem mass spectrometry$94050037 997 $aUNINA