05400nam 22006734a 450 991014358260332120170809162043.01-280-72166-997866107216650-470-08795-10-470-08794-3(CKB)1000000000355408(EBL)281847(OCoLC)476027151(SSID)ssj0000174659(PQKBManifestationID)11171696(PQKBTitleCode)TC0000174659(PQKBWorkID)10174632(PQKB)10516282(MiAaPQ)EBC281847(EXLCZ)99100000000035540820060523d2007 uy 0engur|n|---|||||txtccrHPLC for pharmaceutical scientists[electronic resource] /edited by Yuri Kazakevich, Rosario LoBruttoHoboken, N.J. Wiley-Intersciencec20071 online resource (1136 p.)Description based upon print version of record.0-471-68162-8 Includes bibliographical references and indexes.HPLC FOR PHARMACEUTICAL SCIENTISTS; CONTENTS; PREFACE; CONTRIBUTORS; PART I HPLC THEORY AND PRACTICE; 1 Introduction; 1.1 Chromatography in the Pharmaceutical World; 1.2 Chromatographic Process; 1.3 Classification; 1.4 History of Discovery and Early Development (1903-1933); 1.5 General Separation Process; 1.5.1 Modern HPLC Column; 1.5.2 HPLC System; 1.6 Types of HPLC; 1.6.1 Normal-Phase Chromatography (NP HPLC); 1.6.2 Reversed-Phase HPLC (RP HPLC or RPLC); 1.6.3 Ion-Exchange Chromatography (IEX); 1.6.4 Size-Exclusion Chromatography (SEC); 1.7 HPLC Descriptors (Vr, k, N, etc.)1.7.1 Retention Volume1.7.2 Void Volume; 1.7.3 Retention Factor; 1.7.4 Selectivity; 1.7.5 Efficiency; 1.7.6 Resolution; References; 2 HPLC Theory; 2.1 Introduction; 2.2 Basic Chromatographic Descriptors; 2.3 Efficiency; 2.4 Resolution; 2.5 HPLC Retention; 2.6 Retention Mechanism; 2.7 General Column Mass Balance; 2.8 Partitioning Model; 2.9 Adsorption Model; 2.10 Total and Excess Adsorption; 2.11 Mass Balance in Adsorption Model; 2.12 Adsorption of the Eluent Components; 2.13 Void Volume Considerations; 2.14 Thermodynamic Relationships; 2.14.1 Effect of the Eluent Composition2.15 Adsorption-Partitioning Retention Mechanism2.16 Secondary Equilibria; 2.16.1 Inclusion of Secondary Equilibria in the Mass Balance; 2.16.2 Salt Effect; 2.17 Gradient Elution Principles; 2.18 Types of Analyte Interactions with the Stationary Phase; 2.19 Conclusion; References; 3 Stationary Phases; 3.1 Introduction; 3.2 Type of Packing Material (Porous, Nonporous, Monolithic); 3.3 Base Material (Silica, Zirconia, Alumina, Polymers); 3.4 Geometry; 3.4.1 Shape (Spherical/Irregular); 3.4.2 Particle Size Distribution; 3.4.3 Surface Area; 3.4.4 Pore Volume; 3.4.5 Surface Geometry3.5 Adsorbent Surface Chemistry3.5.1 Surface Chemistry of the Base Material; 3.5.2 Silica; 3.5.3 Silica Hybrid; 3.5.4 Polymeric Packings; 3.5.5 Zirconia (Metal Oxides); 3.5.6 Porous Carbon (or Carbon-Coated Phases); 3.6 Surface of Chemically Modified Material; 3.6.1 Limits of Surface Modification; 3.6.2 Chemical Modification; 3.6.3 Types of Bonded Phases; 3.6.4 Structure of the Bonded Layer; 3.6.5 Density of Bonded Ligands; 3.6.6 Residual Silanoles; 3.6.7 Surface Area of Modified Adsorbent; 3.7 Polymer-Based Adsorbents; 3.8 Stationary Phases for Chiral Separations3.8.1 Polysaccharide-Coated Phases3.8.2 Pirkle-Type Phases; 3.8.3 Protein Phases; 3.8.4 Molecular Imprinted Polymers for Chiral Separations; 3.9 Columns; 3.9.1 Capillary/Monolithic/Packed Columns; 3.9.2 Column Cleaning; 3.9.3 Column Void Volume; 3.9.4 Mass of Adsorbent in the Column; References; 4 Reversed-Phase HPLC; 4.1 Introduction; 4.2 Retention in Reversed-Phase HPLC; 4.3 Stationary Phases for RPLC; 4.4 Mobile Phases for RPLC; 4.4.1 Eluent Composition and Solvent Strength of the Mobile Phase; 4.4.2 Type of Organic Modifier4.4.3 Selectivity as a Function of Type and Concentration of Organic CompositionHPLC for Pharmaceutical Scientists is an excellent book for both novice and experienced pharmaceutical chemists who regularly use HPLC as an analytical tool to solve challenging problems in the pharmaceutical industry. It provides a unified approach to HPLC with an equal and balanced treatment of the theory and practice of HPLC in the pharmaceutical industry. In-depth discussion of retention processes, modern HPLC separation theory, properties of stationary phases and columns are well blended with the practical aspects of fast and effective method development and method validation. PrHigh performance liquid chromatographyDrugsAnalysisClinical chemistryElectronic books.High performance liquid chromatography.DrugsAnalysis.Clinical chemistry.615.19615.1901615/.1901Kazakevich Yuri922091LoBrutto Rosario922092MiAaPQMiAaPQMiAaPQBOOK9910143582603321HPLC for pharmaceutical scientists2069203UNINA01733nam0 2200361 i 450 CFI007389820251003044118.0IT88-10570 20160402d1987 ||||0itac50 baitaitz01i xxxe z01nz01ncRDAcarrierˆLa ‰città modernaitinerario storico di idee ed esperienzePaolo Vitolonota introduttiva di Mario ColettaNapoliCUEN1987XV,171 p.ill.23 cm.UrbanisticaSec. 18.-20.FIRCFIC025602I711PIANIFICAZIONE TERRITORIALE (URBANISTICA)20711.409PIANI E PIANIFICAZIONE MUNICIPALE.STORIA GEOGRAFIA PERSONE21711.409URBANISTICA. PIANI E PIANIFICAZIONE MUNICIPALE. Storia, geografia, persone23Vitolo, PaoloCFIV0469120705649Coletta, MarioCFIV046915ITIT-00000020160402IT-BN0095 IT-NA0079 IT-BN0015 NAP BNMAGAZZINO magazzini correnti divisi per anniNAP PBGENERALE Dizionari, Enciclopedie, AtlantiNAP 01POZZO LIB.Vi sono collocati fondi di economia, periodici di ingegneria e scienze, periodici di economia e statistica e altri fondi comprendenti documenti di economia pervenuti in dono. CFI0073898Biblioteca Centralizzata di Ateneo1 v. 01POZZO LIB.ECON MON 7116 0101 0700202745E VMA 1 v. (Precedente collocazione 20 Ec 274)B 2022072820220728 01 BN PBCittà moderna618175UNISANNIO