04184nam 2200649 450 99630875140331620221206172600.01-5231-0070-23-11-042625-03-11-041133-410.2478/9783110411331(CKB)3710000000346226(SSID)ssj0001535743(PQKBManifestationID)11835763(PQKBTitleCode)TC0001535743(PQKBWorkID)11502844(PQKB)11276661(MiAaPQ)EBC1787150(DE-B1597)445659(OCoLC)1013967206(OCoLC)954878217(DE-B1597)9783110411331(Au-PeEL)EBL1787150(CaPaEBR)ebr11054948(OCoLC)939262791(EXLCZ)99371000000034622620150531h20142014 uy 0engurcnu||||||||txtccrBiochemistry laboratory manual for undergraduates an inquiry-based approach /Timea Gerczei, Scott Pattison; managing editor, Anna RulkaWarsaw, [Poland] ;Berlin, [Germany] :De Gruyter Open,2014.©20141 online resource (186 pages) illustrations, (some colour)Bibliographic Level Mode of Issuance: Monograph3-11-041132-6 Front matter --Preface --Contents --1 Introducing the Bacterial Antibiotic Sensor Mini Project --2 Identifying Conserved Elements in the Toxin Sensor and Designing Mutants to Test Whether They are Important for Function --3 Designing Primers for Site-Directed Mutagenesis --4 Performing Site-Directed Mutagenesis --5 Purifying Mutant Toxin Sensor DNA from Bacterial Cells and Evaluating its Quality Using Agarose Gel Electrophoresis and UV Spectroscopy --6 Preparing DNA Template for Mutant RNA Sensor Synthesis Using a Restriction Endonuclease --7 Synthesizing the ykkCD Mutant Toxin Sensor RNA in vitro --8 Purifying the ykkCD Mutant Toxin Sensor RNA and Evaluating its Purity Using Denaturing PAGE and UV spectrometry --9 Evaluating the Ability of the ykkCD Toxin Sensor to Recognize the Antibiotic Tetracycline Using Fluorescent Quenching --10 Evaluating Antibiotic Binding to Blood Serum Albumin Using Fluorescence Spectroscopy --11 Understanding the Importance of Buffers in Biological Systems --12 Molecular Visualization of an Enzyme, Acetylcholinesterase --13 Determining the Efficiency of the Enzyme Acetylcholine Esterase Using Steady-State Kinetic Experiment --14 Separation of the Phosphatidylcholines Using Reverse Phase HPLC --List of FiguresBiochemistry laboratory manual for undergraduates - an inquiry based approach by Gerczei and Pattison is the first textbook on the market that uses a highly relevant model, antibiotic resistance, to teach seminal topics of biochemistry and molecular biology while incorporating the blossoming field of bioinformatics. The novelty of this manual is the incorporation of a student-driven real real-life research project into the undergraduate curriculum. Since students test their own mutant design, even the most experienced students remain engaged with the process, while the less experienced ones get their first taste of biochemistry research. Inclusion of a research project does not entail a limitation: this manual includes all classic biochemistry techniques such as HPLC or enzyme kinetics and is complete with numerous problem sets relating to each topic.BiochemistryLaboratory manualsMolecular biologyLaboratory manualsDrug resistance in microorganismsLaboratory manualsBiochemistryMolecular biologyDrug resistance in microorganisms572.078Gerczei Timea987175Pattison Scott1947-,Rulka AnnaMiAaPQMiAaPQMiAaPQBOOK996308751403316Biochemistry laboratory manual for undergraduates2256096UNISA