04173nam 2200661Ia 450 991045777120332120200520144314.01-283-31097-X97866133109720-12-385545-4(CKB)2550000000063471(EBL)802466(OCoLC)769342081(SSID)ssj0000591978(PQKBManifestationID)11352048(PQKBTitleCode)TC0000591978(PQKBWorkID)10728666(PQKB)11568203(MiAaPQ)EBC802466(PPN)170261727(Au-PeEL)EBL802466(CaPaEBR)ebr10509200(CaONFJC)MIL331097(EXLCZ)99255000000006347120111109d2012 uy 0engurcn|||||||||txtrdacontentstirdacontentcrdamediacrrdacarrierMolecular biology techniques[electronic resource] a classroom laboratory manual /Susan Carson, Heather Miller, D. Scott Witherow3rd ed.Amsterdam ;Boston Elsevier /Academic Pressc20121 online resource (xxvi, 200 pages) color illustrationsIncludes index.Previous edition: 2006.0-12-385544-6 Includes bibliographical references and index.Lab Session 1 Getting Oriented: Practicing with Micropipettes -- Lab Session 2 Purification and Digestion of Plasmid (Vector) DNA -- Lab Session 3 PCR Amplification of egfp and Completion of Vector Preparation -- Lab Session 4 Preparation of Insert DNA (egfp) PCR Product -- Lab Session 5 DNA Ligation and Transformation of Escherichia coli -- Lab Session 6 Colony Hybridization -- Lab Session 7 Characterization of Recombinant Clones: Part 1 -- Lab Session 8 Characterization of Recombinant Clones: Part 2 -- Lab Session 9 Characterization of Recombinant Clones: Part 3 -- Lab Session 10 Computational Analysis of DNA Sequence from a Positive Clone: Part 2 -- Lab Session 11 Expression of Fusion Protein from Positive Clones, SDS-PAGE and Western Blot: Part 1 -- Lab Session 12 Expression of Fusion Protein from Positive Clones, SDS-PAGE and Western Blot: Part 2 -- Lab Session 13 Extraction of Recombinant Protein from Escherichia coli Using a Glutathione Affinity Column -- Lab Session 14 Analysis of Purification Fractions -- Lab Session 15 Total RNA Purification -- Lab Session 16 Analysis of gst::egfp mRNA Levels by RT-qPCR: Part 1 -- Lab Session 17 Analysis of gst::egfp mRNA Levels by RT-qPCR: Part 2 -- Lab Session 18 Analysis of gst::egfp mRNA Levels by Semi-Quantitative RT-PCR: Part 1 -- Lab Session 19 Analysis of gst::egfp mRNA Levels by Semi-Quantitative RT-PCR: Part 2 -- Appendix 1 Equipment -- Appendix 2 Prep List -- Appendix 3 Preparation of Competent E. coli Cells -- Appendix 4 Pre-Lab Questions.This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein.The second edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester,Molecular biologyLaboratory manualsMolecular biologyTechniqueHandmooks, manuals, etcElectronic books.Molecular biologyMolecular biologyTechnique572.8/078572.8078Carson SueDr.988962Miller Heather Bennett988963Witherow D. Scott(Dennis Scott)988964MiAaPQMiAaPQMiAaPQBOOK9910457771203321Molecular biology techniques2261540UNINA03921nam 2200661 450 991046577750332120200917021826.03-11-047589-83-11-047713-010.1515/9783110477139(CKB)3710000000718469(EBL)4605640(SSID)ssj0001681623(PQKBManifestationID)16507147(PQKBTitleCode)TC0001681623(PQKBWorkID)15026097(PQKB)11562674(PQKBManifestationID)16494768(PQKBWorkID)15026098(PQKB)22152022(MiAaPQ)EBC4605640(DE-B1597)465955(OCoLC)951916038(DE-B1597)9783110477139(Au-PeEL)EBL4605640(CaPaEBR)ebr11238670(CaONFJC)MIL941830(OCoLC)954285129(EXLCZ)99371000000071846920160820h20162016 uy 0engur|n|---|||||txtccrLevitical sacrifice and heavenly cult in Hebrews /Benjamin J. RibbensBerlin, [Germany] ;Boston, [Massachusetts] :De Gruyter,2016.©20161 online resource (316 p.)Beihefte zur Zeitschrift für die neutestamentliche Wissenschaft,0171-6441 ;Volume 222Description based upon print version of record.3-11-047581-2 Includes bibliographical references and indexes.Frontmatter -- Acknowledgements -- Contents -- List of Abbreviations -- 1. The Efficacy of Levitical Sacrifice Compared to Christ’s Sacrifice in Hebrews -- 2. Sacrifice Theology in Second Temple Judaism -- 3. Heavenly Cult in Second Temple Judaism -- 4. Heavenly Tabernacle and Cult in Hebrews -- 5. Old Covenant Sacrifices -- 6. New Covenant Sacrifice -- 7. Conclusion -- Appendix: Perfection in Second Temple Literature and the New Testament -- Bibliography -- Index of References -- Index of Authors -- Index of Subjects This monograph examines Hebrews’ understanding of the relationship between old covenant sacrifices and Christ’s new covenant sacrifice, especially as it relates to the question of efficacy. Most scholars think the author of Hebrews strips the levitical sacrifices of most, if not all, efficacy, but this work affirms a more positive depiction of the levitical sacrifices. A mystical apocalyptic tradition stands behind Hebrews’ description of the heavenly cult , which establishes the framework for relating the levitical sacrifice to Christ’s sacrifice. The earthly, levitical cult was efficacious when it corresponded to or synchronized with the heavenly sacrifice of Christ. Still, the author of Hebrews develops the notion of the heavenly cult in unique ways, as Christ’s sacrifice both validates the earthly practice but also, due to his new covenant theology, calls for its end. Ribbens’ bold proposal joins a growing number of scholars that place Hebrews in the mystical apocalyptic tradition, highlights positive statements in Hebrews related to the efficacy of levitical sacrifices that are often overlooked, and relies on the heavenly cult to reconcile the positive and negative descriptions of the levitical cult.Beihefte zur Zeitschrift für die neutestamentliche Wissenschaft und die Kunde der älteren Kirche ;Volume 222.SacrificeBible teachingElectronic books.SacrificeBible teaching.227/.8706Ribbens Benjamin J.1983-1056123MiAaPQMiAaPQMiAaPQBOOK9910465777503321Levitical sacrifice and heavenly cult in Hebrews2490234UNINA01214nam0 22002891i 450 UON0038872020240520093406.81420110218d1968 |0itac50 baengUS|||| 1||||Essays on English and American literatureby Leo Spitzeredited by Anna Hatcher PrincetonPrinceton University Press1968XVII, 290 p.21 cm.001UON003887212001 Princeton Paperbacks.Literature210 PrincetonPrinceton University PressLetteratura americanaSaggiUONC077383FILETTERATURA INGLESESaggiUONC077382FIUSPrincetonUONL000074SpitzerLeoUONV0619980HATCHERAnnaUONV200167Princeton University PressUONV245813650ITSOL20251003RICASIBA - SISTEMA BIBLIOTECARIO DI ATENEOUONSIUON00388720SIBA - SISTEMA BIBLIOTECARIO DI ATENEOSI Angl XI 051 SI SI 3468 5 051 Essays on english and american literature175134UNIOR