04301nam 22004335 450 991056309150332120190412143002.01-4939-7021-610.1007/978-1-4939-7021-6(CKB)4340000000062474(DE-He213)978-1-4939-7021-6(OCoLC)1028534965(PPN)202988481(EXLCZ)99434000000006247420170620d2017 u| 0engurnn#008mamaatxtrdacontentcrdamediacrrdacarrier3D Cell Culture[electronic resource] Methods and Protocols /edited by Zuzana Koledova1st ed. 2017.New York, NY :Springer New York :Imprint: Humana,2017.1 online resource (XVI, 452 p. 114 illus., 99 illus. in color.)Methods in Molecular Biology,1064-3745 ;16121-4939-7019-4 3D Cell Culture: An Introduction -- Preparation of Decellularized Biological Scaffolds for 3D Cell Culture -- 3D Cell Culture in Interpenetrating Networks of Alginate and rBM Matrix -- Hydrogel-Based In Vitro Models of Tumor Angiogenesis -- Generation of Induced Pluripotent Stem Cells in Defined Three-Dimensional PEG Hydrogels -- Calcium Phosphate Foams: Potential Scaffolds for Bone Tissue Modelling in Three-Dimensions.oids -- 3d="" fluid-dynamic="" models="" multiple="" myeloma -- preparation="" three-dimensional="" full="" thickness="" skin="" equivalent -- analysis="" breast="" cell="" invasion="" using="" an="" system -- 3d="" the="" brain="" parenchyma="" metastasis="" interface="" metastasis -- 3d="" neural="" in="" dual="" hydrogel="" systems -- 3d="" micro-patterned="" hydrogels="" prepared="" by="" photomask,="" micro-needle,="" or="" soft="" lithography="" techniques -- 3d="" niche="" engineering="" via="" two-photon="" laser="" polymerization -- microfluidic-based="" generation="" collagen="" spheres="" investigate="" multicellular="" spheroid="" invasion -- high-throughput="" sphere="" formation="" culture -- high-throughput="" tumor="" recyclable="" microfluidic="" platform -- high-throughput="" platform="" mesenchymal="" anastomosed="" microvascular="" network="" living="" capillary="" networks="" and="" endothelial="" cell-lined="" channels -- human="" small="" airway-on-a-chip="" protocol -- microfluidic="" bioprinting="" heterogeneous="" tissue="" constructs -- bioprinting="" decellularized="" extracellular="" matrix="" bioink -- bioprinting="" cartilage="" peg="" hydrogel -- real-time="" cycle="" imaging="" melanoma -- revealing="" ultrastructure="" morphology="" spheroids="" electron="" microscopy -- quantitative="" phenotypic="" image="" analysis="" cultures.This book provides an overview of established 3D cell culture assays from leaders in the field. Their contributions cover a wide spectrum of techniques and approaches for 3D cell culture, from organoid cultures through organotypic models to microfluidic approaches and emerging 3D bioprinting techniques, which are used in developmental, stem cell, cancer, and pharmacological studies, among many others. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, 3D Cell Culture: Methods and Protocols aims to inspire researchers to develop novel 3D cell culture techniques according to their specific scientific needs and interests, leading to a new generation of physiologically relevant and realistic 3D cell cultures. Chapter 15 of this book is available open access under a CC BY 4.0 license.Methods in Molecular Biology,1064-3745 ;1612CytologyCell Biologyhttp://scigraph.springernature.com/things/product-market-codes/L16008Cytology.Cell Biology.571.6Koledova Zuzanaedthttp://id.loc.gov/vocabulary/relators/edtBOOK99105630915033213D Cell Culture2835991UNINA00781nam a2200229 i 450099100239476970753620020508195500.0931217s1930 it ||| | ita b11002785-39ule_instPARLA161729ExLDip.to FilosofiaitaSand, George137342Consuelo :Romanzo /Giorgio SandMilano :Fratelli Treves,19303 v. ;24 cm.Ripr. facsimilare..b1100278521-09-0628-06-02991002394769707536LE005 MF 23 M 3/I-3/III1LE005N-9965/9965/IIle005-E0.00-l- 00000.i1111931728-06-02Consuelo193209UNISALENTOle00501-01-93ma -itait 0101081nam0 22002651i 450 UON0038040820231205104525.12120100420d1817 |0itac50 baitaIT||||||||| |||||||||Avventure e osservazioni di Filippo Pananti sopra le coste di Barberia. Prima parteFirenzePresso Leonardo Ciardetti18171 v.21 cmBERBERIStoriaDocumentiUONC081097FIITFirenzeUONL000052961.004933Storia dei berberi21PanantiFilippoUONV089049221992Leonardo CiardettiUONV277245650ITSOL20250620RICASIBA - SISTEMA BIBLIOTECARIO DI ATENEOUONSIUON00380408SIBA - SISTEMA BIBLIOTECARIO DI ATENEOSI * 022 SI AA 30157 6 022 Avventure e osservazioni di Filippo Pananti sopra le coste di Barberia. Prima parte1356640UNIOR