05211nam 2200649Ia 450 991101924930332120200520144314.09786611239268978128123926612812392679783527612307352761230097835276123143527612319(CKB)1000000000375888(EBL)482134(OCoLC)609855542(SSID)ssj0000303617(PQKBManifestationID)11263557(PQKBTitleCode)TC0000303617(PQKBWorkID)10275996(PQKB)11526391(MiAaPQ)EBC482134(Perlego)2772077(EXLCZ)99100000000037588820050323d2005 uy 0engur|n|---|||||txtccrMore practical problem solving in HPLC /Stavros Kromidas ; with contributions by Friedrich Mandel, Jurgen Maier-Rosenkranz and Hans-Joachim KussWeinheim ;[Great Britain] Wiley-VCHc20051 online resource (312 p.)Includes index.9783527311132 3527311130 More Practical Problem Solving in HPLC; Foreword; Contents; Preface; The Structure of the Book; Part 1 (general section); Part 2 (specific questions); In Lieu of an Introduction; Chromatography - and more - Crossword; Across; Down; An HPLC-Quiz; An HPLC Tale; The Tale of Peaky and Chromy; 1 HPLC Tips; 1.1 Stationary Phases and Columns; Tip No. 01 "It improves with age" is a rule that applies to port and sometimes to red wine, but how about your C(18) column?; Tip No. 02 Optimization via column parameters - what works best?Tip No. 03 Can selectivity always be put down to chemical interactions with the stationary phase?Tip No. 04 A matter of perspective . . . Or: Selectivity and peak symmetry of basic compounds using reversed-phase packing materials; Tip No. 05 Separation of isomers; Tip No. 06 When should I use a "polar" C(18) phase?; Tip No. 07 Are polar RP-C(18) phases more suitable for the separation of polar analytes than non-polar phases?; Tip No. 08 What about non-endcapped phases - are they a thing of the past?; Tip No. 09 How can I separate acids using RP C(18)?Tip No. 10 The nitrile phase - some like it polarTip No. 11 The selectivity of RP columns; 1.2 Buffers, pH Value; Tip No. 12 Does it always have to be potassium phosphate?; Tip No. 13 UV cut-off of buffer solutions; Tip No. 14 Sources of errors when using buffers; Tip No. 15 The drawbacks of using buffers; Tip No. 16 Why is the pH value so important, and what does it do?; Tip No. 17 Why does the pH value shift even though I am using the correct buffer and the buffer capacity is sufficient?; Tip No. 18 Changes to the pH value in the eluent: the extent of the shift and the reasons behind itTip No. 19 An unintentional pH shift and its consequencesTip No. 20 RP separations in the alkaline medium; Tip No. 21 Separation of basic and acidic compounds contained in the same sample; 1.3 Optimization, Peak Homogeneity; Tip No. 22 The peaks appear too soon - what can be done?; Tip No. 23 What can I do if the peaks elute late?; Tip No. 24 Quick optimization of an existing gradient method; Tip No. 25 Increasing efficiency - often the fast track to success; Tip No. 26 Additives to the eluent; Tip No. 27 Separating the unknown - where shall I begin?Tip No. 28 Separation of an unknown sample using a reversed-phase C(18) column - how do I go about it?Tip No. 29 Developing an RP separation - the two-day-method; Part 1: Choice of column and eluent; Tip No. 30 Developing an RP separation - the two-day method; Part 2: Fine-tuning of the separation; Tip No. 31 Quick check on peak homogeneity; Tip No. 32 Quick check on peak homogeneity; Tip No. 33 Tied to a standard operating procedure - how can a bad separation be improved further?; Tip No. 34 More elaborate measures to check peak homogeneity; Tip No. 35 First easily digestible tipTip No. 36 Second easily digestible tipA unique approach to solving HPLC problems.Everyone who bought ""Problem Solving in HPLC"" by Stavros Kromidas will equally benefit from nearly 100 new practical examples for optimization, trouble-shooting, and instrument performance given in this sequel.The author provides- guidance for selecting and evaluating methods, intstruments and columns,- practical help with everyday trouble-shooting,- advice for optimizing separations, always explaining the reason why.In each case the problem, the solution and the conclusions are presented over a maximum of 4 pages, and in addHigh performance liquid chromatographyPhase partitionHigh performance liquid chromatography.Phase partition.543.84Kromidas Stavros1604367MiAaPQMiAaPQMiAaPQBOOK9911019249303321More practical problem solving in HPLC4421112UNINA