03554nam 2200685Ia 450 991045103950332120210603204035.01-281-12546-697866111254620-226-14400-310.7208/9780226144009(CKB)1000000000411228(EBL)408589(OCoLC)476229779(SSID)ssj0000251800(PQKBManifestationID)11206770(PQKBTitleCode)TC0000251800(PQKBWorkID)10175753(PQKB)11556014(StDuBDS)EDZ0000119067(MiAaPQ)EBC408589(DE-B1597)524364(OCoLC)1055416647(DE-B1597)9780226144009(Au-PeEL)EBL408589(CaPaEBR)ebr10210003(CaONFJC)MIL112546(EXLCZ)99100000000041122820021114d2003 uy 1engurun#---|u||utxtccrThe story of Sapho[electronic resource] /Madeleine de Scudéry ; translated and with an introduction by Karen NewmanChicago University of Chicago Press20031 online resource (189 p.)The other voice in early modern EuropeDescription based upon print version of record.0-226-14399-6 0-226-14398-8 Includes bibliographical references (p. 145-155).Front matter --CONTENTS --Acknowledgments --Series Editors' Introduction --Volume Editor's Introduction --The Story of Sapho --The Twentieth Harangue from Les femmes illustres; ou, Les harangues héröıques --Volume Editor's Bibliography --Series Editors' BibliographyRidiculed for her Saturday salon, her long romance novels, and her protofeminist ideas, Madeleine de Scudéry (1607-1701) has not been treated kindly by the literary establishment. Yet her multivolume novels were popular bestsellers in her time, translated almost immediately into English, German, Italian, Spanish, and even Arabic. The Story of Sapho makes available for the first time in modern English a self-contained section from Scudéry's novel Artamène ou le Grand Cyrus, best known today as the favored reading material of the would-be salonnières that Molière satirized in Les précieuses ridicules. The Story tells of Sapho, a woman writer modeled on the Greek Sappho, who deems marriage slavery. Interspersed in the love story of Sapho and Phaon are a series of conversations like those that took place in Scudéry's own salon in which Sapho and her circle discuss the nature of love, the education of women, writing, and right conduct. This edition also includes a translation of an oration, or harangue, of Scudéry's in which Sapho extols the talents and abilities of women in order to persuade them to write.Other voice in early modern Europe.French literature17th centuryTranslations into EnglishWomenFranceSocial conditions17th centuryFictionElectronic books.French literatureWomenSocial conditions843/.7Scudéry Madeleine de1607-1701.403267Newman Karen1949-887391MiAaPQMiAaPQMiAaPQBOOK9910451039503321The story of Sapho2216440UNINA05172nam 22006974a 450 991101909890332120200520144314.0978661194699997835276915003527691502978128194699712819469909783527622702352762270597835276226963527622691(CKB)1000000000550884(EBL)481824(SSID)ssj0000119208(PQKBManifestationID)11144005(PQKBTitleCode)TC0000119208(PQKBWorkID)10057867(PQKB)10715635(MiAaPQ)EBC481824(OCoLC)264717199(Perlego)1009038(EXLCZ)99100000000055088420090404d2008 uy 0engur|n|---|||||txtccrCell-free protein synthesis methods and protocols /edited by Alexander S. Spirin and James R. SwartzWeinheim Wiley-VCHc20081 online resource (264 p.)Description based upon print version of record.9783527316496 3527316493 Includes bibliographical references and index.Cell-free Protein Synthesis; Contents; Preface; List of Contributors; 1 Cell-free Protein Synthesis Systems: Historical Landmarks, Classification, and General Methods; 1.1 Introduction: Historical Landmarks; 1.1.1 Discovery of Protein Synthesis in Cell Extracts; 1.1.2 Translation of Exogenous Messages; 1.1.3 Coupled Transcription-translation in Bacterial Extracts; 1.1.4 Combined Transcription-translation Systems; 1.1.5 Continuous Flow/Continuous Exchange Principle; 1.2 Prokaryotic and Eukaryotic Types of Cell-free Expression Systems; 1.2.1 Cell Extracts; 1.2.1.1 E. coli extract (ECE)1.2.1.2 Wheat Germ Extract (WGE)1.2.1.3 Rabbit Reticulocyte Lysate (RRL); 1.2.2 Genetic Constructs (Expression Vectors); 1.2.2.1 Prokaryotic Systems; 1.2.2.2 Eukaryotic Systems; 1.3 Preparing Cell Extracts; 1.3.1 E. coli Extracts; 1.3.1.1 Genetics; 1.3.1.2 Cell Growth; 1.3.1.3 Extract Preparation; 1.3.2 Wheat Germ Extracts; 1.4 Designing Reaction Composition; 1.4.1 Mg(2+) and Phosphate; 1.4.2 Other Salts; 1.4.3 Nucleotides and Amino Acids; 1.4.4 Stabilization Reagents; 1.4.5 Other Factors; 1.5 Providing Energy; 1.5.1 Direct Nucleotide Regeneration; 1.5.2 Indirect Nucleotide Regeneration1.6 Enhancing Protein Folding1.6.1 Temperature Effects; 1.6.2 Cell Extract Concentration; 1.6.3 Effects of Folding Ligands; 1.6.4 Effects of Chaperones and Foldases; 1.6.5 Effects of Detergents; 2 The Constructive Approach for Cell-free Translation; 2.1 Introduction; 2.2 The Process of Protein Synthesis; 2.2.1 Polypeptide Synthesis; 2.2.2 Protein Maturation; 2.3 A Constructive Approach to Protein Synthesis; 2.3.1 In Vitro Reconstitution of Polypeptide Synthesis; 2.3.2 Protocol of Protein Synthesis using PURE System; 2.3.3 Addition of Protein Folding Machinery to the PURE System2.3.4 Integration of a Membrane Targeting System with the PURE system2.3.5 Protein Synthesis using the PURE System containing Molecular Chaperones; 2.4 Conclusion; 3 Functional Genomic Analysis using Sequential Cell-free Protein Synthesis; 3.1 Introduction; 3.1.1 The Post-genomic Era; 3.1.2 Cell-free Protein Synthesis (CFPS) as a Functional Proteomic Tool; 3.2 Developing an enabling Technology for Sequential Expression Analysis; 3.2.1 Improving Linear Template Stability; 3.2.2 Improving PCR Reactions for generating Genomic Linear Templates3.2.3 Optimizing Cofactor Concentrations for Enzyme Activation3.3 Demonstrating Functional Genomic Analysis with CFPS; 3.3.1 Isolation and Expression of Genomic Targets; 3.3.2 Effects of Sample Library on β-Lactamase Expression and Activity; 3.4 Conclusions and Projections; 4 Cell-free Technology for Rapid Production of Patient-specific Fusion Protein Vaccines; 4.1 Introduction; 4.1.1 Lymphoma and Fusion Protein Vaccine Treatments; 4.1.2 Comparing Cell-free and In Vivo Production Systems; 4.2 Developing the Fusion Protein Construct and the Cell-free Production Process4.2.1 Fusion-protein Production in the Cell-free SystemWith its detailed description of membrane protein expression, high-throughput and genomic-scale expression studies, both on the analytical and the preparative scale, this book covers the latest advances in the field. The step-by-step protocols and practical examples given for each method constitute practical advice for beginners and experts alike.ProteinsSynthesisGenetic translationProteinsSynthesis.Genetic translation.572.6Spirin A. S(Aleksandr Sergeevich)64527Swartz James R1841541MiAaPQMiAaPQMiAaPQBOOK9911019098903321Cell-free protein synthesis4421300UNINA