00825nam0-22002891i-450-99000086175040332120081127152246.00070522081000086175FED01000086175(Aleph)000086175FED0100008617520020821d1983----km-y0itay50------baitay-------001yyNumerical Methods, Software, and AnalysisJ.R. RiceAucklandMcGraw-Hill Book Company1983XII, 483 p.26 cm519.4Rice,John R.<Rischard ;1934- >333432ITUNINARICAUNIMARCBK99000086175040332102 47 E 66042FINBNFINBNNumerical methods, software, and analysis114797UNINA02953nam 2200625Ia 450 991095365360332120200520144314.09781462508235146250823597814625082281462508227(CKB)2670000000336940(EBL)1137444(OCoLC)828792721(SSID)ssj0000833070(PQKBManifestationID)12299401(PQKBTitleCode)TC0000833070(PQKBWorkID)10935486(PQKB)10810256(MiAaPQ)EBC1137444(Perlego)4409722(EXLCZ)99267000000033694020121128d2013 uy 0engur|n|---|||||txtccrDoing statistical mediation and moderation /Paul E. JoseNew York Guilford Pressc20131 online resource (354 p.)Methodology in the social sciencesDescription based upon print version of record.9781462508211 1462508219 9781462508150 1462508154 Includes bibliographical references and index.Cover; Front Matter; Series Page; Title Page; Copyright; Dedication; Series Editor's Note; Preface; Acknowledgments; Contents; 1--A Basic Orientation; 2--Historical Background; 3--Basic Mediation; 4--Special Topics in Mediation; 5--Basic Moderation; 6--Special Topics in Moderation; 7--Mediated Moderation and Moderated Mediation; Appendix A--SPSS, Amos, and Mplus Models; Appendix B--Resources for Researchers Who Use Mediation and Moderation; References; Author Index; Subject Index; About the Author Written in a friendly, conversational style, this book offers a hands-on approach to statistical mediation and moderation for both beginning researchers and those familiar with modeling. Starting with a gentle review of regression-based analysis, Paul Jose covers basic mediation and moderation techniques before moving on to advanced topics in multilevel modeling, structural equation modeling, and hybrid combinations, such as moderated mediation. User-friendly features include numerous graphs and carefully worked-through examples; ""Helpful Suggestions"" about procedures and pitfallsMethodology In The Social SciencesMediation (Statistics)Social sciencesStatistical methodsMediation (Statistics)Social sciencesStatistical methods.519.5/36PSY032000EDU027000MED058200BUS061000bisacshJose Paul E(Paul Easton),1952-1809782MiAaPQMiAaPQMiAaPQBOOK9910953653603321Doing statistical mediation and moderation4360736UNINA05549nam 2200721Ia 450 991101893380332120200520144314.09786612689796978128268979412826897979783527627196352762719797835276272023527627200(CKB)1000000000789627(EBL)481654(OCoLC)441887034(SSID)ssj0000342480(PQKBManifestationID)11243285(PQKBTitleCode)TC0000342480(PQKBWorkID)10285626(PQKB)10182309(MiAaPQ)EBC481654(PPN)152543341(Perlego)2767575(EXLCZ)99100000000078962720090617d2009 uy 0engur|n|---|||||txtccrRNA purification and analysis sample preparation, extraction, chromatography /by Douglas T. Gjerde, Lee Hoang and David HornbyWeinheim Wiley-VCH ;Chichester John Wiley [distributor]c20091 online resource (209 p.)Description based upon print version of record.9783527321162 3527321160 Includes bibliographical references and index.RNA Purification and Analysis: Sample Preparation, Extraction, Chromatography; Contents; Preface; Acknowledgments; 1: RNA Extraction, Separation, and Analysis; 1.1 The Need to Be Able to Extract, Manipulate, and Analyze RNA; 1.2 Using Chemical Tools to Solve the Problem of Analysis of Biological Processes; 1.3 The Principle of Chromatography and Solid-Phase Extraction; 1.3.1 Principle of Chromatography; 1.3.2 Mobile Phase Gradient Controls Elution; 1.3.3 Different Types of Column and Eluent Chemistries; 1.3.4 The Principle of Solid-Phase Extraction; 1.4 RNA Chromatography1.5 Enzymatic Treatment of RNA and Analysis1.5.1 Polyacrylamide Gel Electrophoresis; 1.5.2 RNA Structure Probing with Ribonuclease Enzymes; 1.6 Content and Organization of This Book; References; 2: Biological and Chemical RNA; 2.1 Why Classify RNA with Biology and Chemistry?; 2.1.1 Chemical Classification of RNA; 2.1.2 Biological Classification of RNA; 2.2 Prokaryotic Cellular RNA; 2.3 Prokaryote Sample Type; 2.3.1 Escherichia coli; 2.3.2 Other Bacteria; 2.4 Eukaryotic Cellular RNA; 2.5 Eukaryote Sample Type; 2.5.1 Yeast; 2.5.2 Other Fungi; 2.5.3 Simple Multicellular Organism2.5.4 Soft Animal2.5.5 Hard Animal; 2.5.6 Plant; 2.5.7 Cell Culture; 2.6 Other Samples; 2.6.1 Virus; 2.6.2 Soil and Rock; 2.7 Synthetic RNA; 2.7.1 Aptamers; 2.7.2 SELEX; 2.7.3 Short Hairpin RNAs; References; 3: RNA Separation: Substrates, Functional Groups, Mechanisms, and Control; 3.1 Solid-Phase Interaction; 3.1.1 Adsorption of Sample Compounds and Sample Matrix Compounds; 3.1.2 Roles of Solid-Phase Substrate and Functional Group; 3.1.3 Correlation of Interaction Type, Functional Group, and Substrate; 3.1.4 RNA Structure and Solid Surface Interaction3.2 The Solid-Phase Substrate and Attachment of Functional Groups3.2.1 Polymeric Resin Substrates; 3.2.2 Porous and Nonporous Polymeric Resins; 3.2.3 Monolith Polymeric Columns; 3.2.4 Functionalization of the Polymer; 3.2.5 Silica-Glass-Based Substrates; 3.2.6 Functionalization of Silica; 3.2.7 Agarose and Cellulose Affinity Substrates; 3.2.8 Dextran and Polyacrylamide Gel Filtration Substrates; 3.3 Reverse-Phase Ion-Pairing Separation Mechanism; 3.4 Ion-Exchange Separation Mechanism; 3.5 Chaotropic Denaturing Interaction Mechanism; 3.6 Hybridization; 3.6.1 SELEX; 3.7 Gel FiltrationReferences4: RNA Extraction and Analysis; 4.1 Transcription; 4.1.1 RNA Catalysis; 4.1.2 RNA-Protein Complex Interactions; 4.1.3 Pre-mRNA Splicing; 4.2 Translation; 4.2.1 Post-Transcriptional Control of Eukaryotic Gene Expression; 4.3 Gene Regulation; 4.3.1 RNA Interference Pathway; 4.3.2 Micro RNAs and Their Role in Gene Regulation; 4.4 Use of siRNA to Investigate Gene Function; References; 5: RNA Chromatography; 5.1 Development of RNA Chromatography; 5.2 RNA Chromatography Instrumentation; 5.2.1 The Column Oven; 5.2.2 Ultraviolet (UV) and Fluorescence Detection; 5.2.3 Fragment Collection5.3 RNA Chromatography ConditionsThis first book on the market covers the many new and important RNA species discovered over the past five years, explaining current methods for the enrichment, separation and purification of these novel RNAs.Building up from general principles of RNA biochemistry and biophysics, this book addresses the practical aspects relevant to the laboratory researcher throughout, while discussing the performance and potential problems of the methods discussed. An appendix contains a glossary with the important terms and techniques used in RNA analysis.By explaining the basic and working principleRNAAnalysisLaboratory manualsRNAPurificationLaboratory manualsRNAAnalysisRNAPurification572.88572.8836574.87/3283Gjerde Douglas T440146Hornby DavidDr.1840239Hoang Lee1840240MiAaPQMiAaPQMiAaPQBOOK9911018933803321RNA purification and analysis4419759UNINA