03939nam 22006375 450 991101563600332120250712074743.01-0716-4124-710.1007/978-1-0716-4124-8(MiAaPQ)EBC32206303(Au-PeEL)EBL32206303(CKB)39625841100041(DE-He213)978-1-0716-4124-8(EXLCZ)993962584110004120250710d2025 u| 0engurcnu||||||||txtrdacontentcrdamediacrrdacarrierBiophysical Chemistry of Proteins An Introduction to Laboratory Methods /by Engelbert Buxbaum2nd ed. 2025.New York, NY :Springer US :Imprint: Springer,2025.1 online resource (911 pages)1-0716-4123-9 Analytical techniques -- Microscopy -- Single molecule techniques -- Preparation of cells and tissues for microscopy -- Principles of optical spectroscopy -- Photometry -- Fluorimetry -- Chemiluminescence -- Electrophoresis -- Immunological methods -- Isotope techniques -- Purification of proteins -- Homogenisation and fractionisation of cells and tissues -- Isolation of organelles -- Precipitation methods -- Chromatography -- Membrane proteins -- Determination of protein concentration -- Cell culture -- Protein modification and inactivation -- General technical remarks -- Amine-reactive reagents -- Thiol- and disulphide reactive reagents -- Reagents for other groups -- Cross-linkers -- Detection methods -- Spontaneous reactions in proteins -- Protein size and shape -- Centrifugation -- Osmotic pressure -- Diffusion -- Viscosity -- Non-resonant interactions with electromagnetic waves -- Protein structure -- Protein sequencing -- Synthesis of peptides -- Protein secondary structure -- Structure of protein-ligand complexes -- 3D-structures -- Folding and unfolding of proteins -- Enzyme kinetics -- Steady-state kinetics -- Leaving the steady state: Analysis of progress curves -- Reaction velocities -- Isotope effects -- Isotope exchange -- Protein-ligand interactions -- General conditions for interpretable results -- Binding equations -- Methods to measure binding equilibria -- Temperature effects on binding equilibrium and reaction rate -- Industrial enzymology -- Industrial enzyme use -- Immobilised enzymes -- Special statistics -- Quality control -- Testing whether or not a model fits the data -- Appendix -- List of symbols -- Greek alphabet -- Properties of electrophoretic buffers -- Bond properties -- Acronyms.This textbook, designed for all scientists interested in protein research, provides a thorough overview of laboratory methods for the biophysical chemistry of proteins. This new edition, completely restructured and expanded for ease of learning, includes sections on analytical techniques, working with proteins, protein size and shape, protein structure, enzyme kinetics, industry enzymology, and a new section on special statistics.ProteinsProteinsSynthesisProteinsBiochemistryChemistryProtein BiochemistryProtein Synthesis and TranslationProteinsBiochemistryChemistryProteins.ProteinsSynthesis.Proteins.Biochemistry.Chemistry.Protein Biochemistry.Protein Synthesis and Translation.Proteins.Biochemistry.Chemistry.572.6Buxbaum Engelbert1061033MiAaPQMiAaPQMiAaPQBOOK9911015636003321Biophysical Chemistry of Proteins4408062UNINA02372oam 22004934a 450 991028643050332120230621140123.0607-628-760-8968-12-0832-3(CKB)4100000006673600(OCoLC)1053888978(MdBmJHUP)muse84889(WaSeSS)IndRDA00125639(oapen)https://directory.doabooks.org/handle/20.500.12854/89803(oapen)doab89803(EXLCZ)99410000000667360019980429d1997 uy 0spaur|||||||nn|ntxtrdacontentcrdamediacrrdacarrierLa transformación económica en la Rusia poscomunistaTatiana Sidorenko1. ed.El Colegio de México1997Mexico, D.F. :El Colegio de Mexico, Centro de Estudios Internacionales,1997.©1997.1 online resource (189 p.)Includes bibliographical references (p. 183-189).Con la desintegración de la URSS a finales de 1991 se inició en Rusia, la antigua república soviética más grande y económicamente más poderosa, un importante proceso de transición de la sociedad comunista a la moderna democracia capitalista, que implica el paso de una economía de planificación centralizada a una de mercado. En esta obra se examinan los resultados y las dificultades del proceso de transformación económica por el cual está pasando actualmente Rusia. Asimismo, la autora analiza las políticas de la estabilización macroeconómica, las reformas sistémicas, cuyo elemento clave es la privatización y la creación de una gama más amplia de una economía de mercado, el desarrollo del sector empresarial y la apertura de la economía nacional hacia el exterior.EconomicsbicsscRussia (Federation)Economic conditions1991-Russia (Federation)Economic policy1991-Electronic books. Economics of specific sectorsEconomics338.947Sidorenko Tatiana1025759MdBmJHUPMdBmJHUPBOOK9910286430503321La transformación económica en la Rusia poscomunista2439495UNINA