02953nam 2200589 a 450 991097045130332120251117091614.097816166861161616686111(CKB)2550000000059457(EBL)3019928(SSID)ssj0000569856(PQKBManifestationID)12235902(PQKBTitleCode)TC0000569856(PQKBWorkID)10587165(PQKB)11395938(MiAaPQ)EBC3019928(Au-PeEL)EBL3019928(CaPaEBR)ebr10674936(OCoLC)923662102(BIP)29194492(EXLCZ)99255000000005945720100322d2010 uy 0engur|n|---|||||txtccrQuantitative immunohistology problems and solutions /Anthony S-Y Leong and Trishe Y-M Leong1st ed.Hauppauge, N.Y. Nova Science Publishers20101 online resource (82 p.)Immunology and immune system disordersDescription based upon print version of record.9781616682613 1616682612 Includes bibliographical references and index.The immunohistological assay : a total test approach -- Pre-analytical variables : tissue preservation, transportation, fixation and tissue processing -- Analytical variables : antigen retrieval, antibodies, detection systems and controls -- Post-analytical phase -- Receptor detection for targeted therapies -- Interobserver variability in quantitative immunohistology.As immunohistochemistry enters its fourth decade as a diagnostic tool, this analytical procedure continues to experience poor reproducibility, albeit in specific situations. The problem becomes more important with the mounting pressure to employ the assay in a quantitative manner for the assessment of therapeutic and prognostic markers in a number of neoplasms. Qualitative applications of immunohistochemistry are well established and continue to increase with the range of sensitive antibodies and detection systems available, however, numerous variables that influence the immunoexpression of proteins in formalin-fixed tissue continue to exist in the pre-analytical and analytical phases of the test procedure. This book emphasizes these problems in detail and suggests possible solutions.Immunology and immune system disorders.Diagnostic immunohistochemistryDiagnostic immunohistochemistry.616.07/56Leong Anthony S.-Y(Anthony Siew-Yin),1945-2011.1493006Leong Trishe Y-M1867452MiAaPQMiAaPQMiAaPQBOOK9910970451303321Quantitative immunohistology4475001UNINA