04400nam 2200685 a 450 991096664040332120250609110044.01-280-04313-X97866100431320-306-46850-610.1007/0-306-46850-6(CKB)111056486601264(EBL)3035626(SSID)ssj0000179699(PQKBManifestationID)11154650(PQKBTitleCode)TC0000179699(PQKBWorkID)10148222(PQKB)10143501(DE-He213)978-0-306-46850-6(MiAaPQ)EBC3035626(Au-PeEL)EBL3035626(CaPaEBR)ebr10052617(CaONFJC)MIL4313(OCoLC)923696399(MiAaPQ)EBC196710(BIP)46129235(BIP)47202525(EXLCZ)9911105648660126419960515d1996 uy 0engur|n|---|||||txtccrInsect cell cultures fundamental and applied aspects /edited by J.M. Vlak ... [et al.]1st ed. 2002.Dordrecht [Netherlands] ;Boston Kluwerc19961 online resource (325 p.)Current applications in cell culture engineering ;vol. 2Description based upon print version of record.0-7923-3403-5 Includes bibliographical references and index.Insect Cell Lines -- Development and characterization of insect cell lines -- New approaches to insect tissue culture -- Transgenic insect cells: mosquito cell mutants and the dihydrofolate reductase gene -- Insect cell physiology -- Insect cell cultivation: growth and kinetics -- Medium design for insect cell culture -- Viruses -- Baculovirus-insect cell interactions -- Replication patterns and cytopathology of cells infected with baculoviruses -- Construction of baculovirus recombinants -- Passage effect of virus infection in insect cells -- Post-translational modifications in insect cells -- Productivity of insect cells for recombinant proteins -- Chaperone and foldase coexpression in the baculovirus-insect cell expression system -- Engineering Aspects -- Shear sensitivity of insect cells -- Insect cell bioreactors -- Perfusion bioreactors for the production of recombinant proteins in insect cells -- Immobilization of insect cells -- Modelling baculovirus infection of insect cells in culture -- Scale up aspects of sparged insect-cell bioreactors -- Oxygen gradients in small and big sparged insect-cell bioreactors -- Downstream processing of insect cell cultures -- Applications -- Parvovirus diagnostics and vaccine production in insect cells -- Classical swine fever virus diagnostics and vaccine production in insect cells -- Production of multidomain complement glycoproteins in insect cells -- Economic and Regulatory Aspects -- Economics of baculovirus-insect cell production systems -- Safety aspects of insect cell culture -- Regulatory issues in the use of insect-cell culture.A comprehensive reference work covering the key issues in insect cell cultures, this text includes 30 review papers on such topics as: cell lines (development, characterisation, physiology, cultivation and medium design); viruses (virus-cell interactions, replication, recombinant construction, infection kinetics, post-translational modification and passage effects); engineering (shear, bioreactors including perfusion, immobilisation, scale-up and modelling, downstream processing); applications; and economics and regulatory aspects.; This text should be useful for cell biologists, biochemists, molecular biologists, virologists, immunologists and other basic and applied disciplines related to cell culture engineering, both academic and industrial.Current applications of cell culture engineering ;v. 2.Insect cell biotechnologyInsectsVirusesInsectsCytologyInsect cell biotechnology.InsectsViruses.InsectsCytology.660/.6Vlak J. M1823107MiAaPQMiAaPQMiAaPQBOOK9910966640403321Insect cell cultures4389586UNINA