02181nam0 2200469 i 450 MIL019393820231121125540.0883508741420031211d1993 ||||0itac50 baitaitz01i xxxe z01nProslogionAnselmo d'Aostacon In difesa dello stolto di Gaunilone di Marmoutiers e Risposta di S. Anselmoa cura di Giovanni ZuanazziBresciaLa scuola©1993143 p.21 cm˜Il œpensieroIn difesa dello stolto (tit. orig.: Liber pro insipiente); Risposta di S. Anselmo (tit. orig.: Liber apologeticus.001CFI00288612001 ˜Il œpensiero2001 Risposta di S. Anselmo.700 0Anselmus : Cantuariensis <santo>CFIV0768480702001 In difesa dello stolto.700 0GauniloLO1V131338070Teologia dogmaticaFIRRMLC079567IDioFIRRMLC096052E212.121Anselmus : Cantuariensis <santo>CFIV076848070159583GauniloLO1V131338Zuanazzi, GiovanniMILV119153Anselmo : d'AostaBVEV136937Anselmus : Cantuariensis <santo>Anselmo : d'Aosta <santo>CFIV085078Anselmus : Cantuariensis <santo>Anselme : de CantorberyNAPV093858Anselmus : Cantuariensis <santo>Anselmus <archivescovo di Canterbury>VEAV037969Anselmus : Cantuariensis <santo>Gaunilone : di MarmoutiersCFIV143049GauniloGauniloneCFIV150890GauniloITIT-0120031211IT-FR0084 IT-FR0017 Biblioteca Del Monumento Nazionale Di MontecassinoFR0084 Biblioteca umanistica Giorgio ApreaFR0017 MIL0193938Biblioteca umanistica Giorgio Aprea 52CIS 12/1901 52VM 0000860175 VM barcode:00081192. - Inventario:5565 FSVMA 2010122020121204 25 52Proslogion550034UNICAS05050nam 22007094a 450 991096178360332120251116173937.097866111211509781281121158128112115097898127083119812708316(CKB)1000000000334182(EBL)312314(OCoLC)476099627(SSID)ssj0000197433(PQKBManifestationID)11172036(PQKBTitleCode)TC0000197433(PQKBWorkID)10154737(PQKB)10767415(MiAaPQ)EBC312314(WSP)00006269(Au-PeEL)EBL312314(CaPaEBR)ebr10188745(CaONFJC)MIL112115(OCoLC)935264139(PPN)168070251(Perlego)849896(BIP)13812284(EXLCZ)99100000000033418220071109d2007 uy 0engur|n|---|||||txtrdacontentcrdamediacrrdacarrierA manual for biochemistry protocols /Markus R. Wenk, Aaron Zefrin Fernandis1st ed.Singapore ;Hackensack, NJ World Scientificc20071 online resourceManuals in biomedical research ;v. 3Includes index.9789812700667 9812700668 Includes bibliographical references and index.Intro -- Contents -- Preface -- List of Figures -- List of Contributors -- A Protein Purification -- Summary -- A.1 Protein Precipitation -- A.2 Column Chromatography -- A.2.1 Ionic Exchange Chromatography -- A.2.2 Size-Exclusion Chromatography -- A.2.3 Affinity Chromatography -- A.2.4 Purification of Recombinant Proteins -- A.2.5 Commercially Prepacked Column Kits -- Notes -- B Protein Analysis -- B.1 Protein Estimation -- B.1.1 Absorbance Assays -- B.1.2 Colourimetric Assays -- B.1.3 Commercial Protein Estimation Kits -- B.2 Spectrometric Analysis -- B.3 SDS-PAGE -- B.4 Gel Staining -- B.4.1 Coomassie Blue Staining -- B.4.2 Silver Staining -- B.5 Western Blotting -- B.6 Immunoprecipitations -- Notes -- C Lipid Analysis -- C.1 Lipid Extraction -- C.1.1 Modified Bligh and Dyers Method for Phospholipid Extraction -- C.1.2 Folch Extraction -- C.1.3 Grey Method for Phosphatidylinositol Phosphate Extraction -- C.1.4 Modified Alex Brown Method for Phosphatidylinositol Phosphate Extraction -- C.1.5 Hexane Extraction for Neutral Lipids -- C.1.6 Glycolipid Extraction -- C.2 Thin Layer Chromatography -- Notes -- D Mammalian Cell Culture -- D.1 Primary Culture -- D.1.1 Primary Cell Isolation and Culturing -- D.1.2 Splenocyte Isolation -- D.1.3 Isolation of Peripheral Blood Lymphocytes -- D.2 Tissue Culture -- D.2.1 Adherent Cells -- D.2.2 Suspension Cells -- D.2.3 Cell Maintenance -- D.3 Cell Count -- D.4 Mammalian Transfection -- D.4.1 Calcium Phosphate Coprecipitation -- D.4.2 Electroporation Protocol -- D.4.3 Lipofectamine Transfection Protocol -- Notes -- E Microscopy -- E.1 Poly-L-Lysine Immobilisation -- E.2 Immunofluorescence and Confocal Microscopy -- E.3 Immunohistochemistry -- Notes -- F Assays -- F.1 Enzyme Assays -- F.1.1 Enzyme Kinetics: Basics -- F.1.2 Lipid Kinase Assay -- F.1.3 Protein Kinase Assay.F.1.4 Protein Tyrosine Phosphatase Assay -- F.1.5 Alkaline Phosphatase Assay -- F.1.6 Caspase Assay -- F.2 Functional Assays -- F.2.1 Apoptosis Assay -- F.2.2 XTT Cell Proliferation Assay [(2,3-Bis (2-methoxy-4-nitro-5-sulfophenyl)- 2H-tetrazolium-5-carboxanilide)] -- F.2.3 Chemotaxis Assay -- F.2.4 Matrigel Invasion or Chemoinvasion Assay -- Notes -- G Autoradiography -- Summary -- Notes -- Suggested Readings -- Abbreviations -- Appendices -- Appendix 1 Safety Issues -- Appendix 2 Buffer Chart -- Appendix 3 Composition of Regular Buffers or Stock Solutions -- Index.Biochemistry plays an important role in all areas of the biological and medical sciences. With most of the research or diagnosis involved in these areas being based on biochemically obtained observations, it is essential to have a profile of well standardized protocols. This manual is a basic guide for all students, researchers and experts in biochemistry, designed to help readers in directly starting off their experiments without prior knowledge of the protocol. The book dwells on the concepts used in designing the methodologies, thereby giving ample room for researchers to modify them according to their research requirements.Manuals in biomedical research ;v. 3.BiochemistryLaboratory manualsBiochemistry572572.078Wenk Markus R1867463Fernandis Aaron Zefrin1867464MiAaPQMiAaPQMiAaPQBOOK9910961783603321A manual for biochemistry protocols4475015UNINA