02721oam 2200637I 450 991095872750332120251117100704.00-429-11045-61-4398-4813-010.1201/b14930 (CKB)2670000000359900(EBL)1190579(SSID)ssj0000876789(PQKBManifestationID)11467572(PQKBTitleCode)TC0000876789(PQKBWorkID)10904439(PQKB)10259615(MiAaPQ)EBC1190579(Au-PeEL)EBL1190579(CaPaEBR)ebr10706067(CaONFJC)MIL502331(OCoLC)845257664(OCoLC)855711925(OCoLC)1280137499(FINmELB)ELB143548(EXLCZ)99267000000035990020180331d2013 uy 0engur|n|---|||||txtccrPCR technology current innovations /edited by Tania Nolan, Stephen A. Bustin3rd ed.Boca Raton, Fla. CRC Press2013Boca Raton :Taylor & Francis,2013.1 online resource (457 p.)Description based upon print version of record.1-4398-4805-X Includes bibliographical references.pt. 1. Template preparation -- pt. 2. Reaction components -- pt. 3. Instruments -- pt. 4. Design, optimization, QC, and standardization -- pt. 5. Data analysis -- pt. 6. Applications.A technique used to amplify the number of copies of a specific region of DNA, the polymerase chain reaction (PCR) is at the forefront of the dramatic development of biochemistry. This text provides the tools for developing innovative approaches to using this leading technology. It includes theoretical considerations, discussions, and a selection of state-of-the-art techniques for mutation studies, clinical diagnosis, and the detection of food-borne pathogens. This edition also discusses the preparation of PCR experiments, includes examples of analytical PCR divided into qualitative and quantitative applications, and explores preparative methods that address DNA generation for further analysis and in vitro evolution--Provided by publisher.DNA polymerasesTransferasesDNA polymerases.Transferases.572.8/636Nolan Tania1876705Bustin Stephen A.1954-1876706MiAaPQMiAaPQMiAaPQBOOK9910958727503321PCR technology4488490UNINA