04438nam 22006255 450 991092044740332120260327113212.09789819768516981976851910.1007/978-981-97-6851-6(MiAaPQ)EBC31867299(Au-PeEL)EBL31867299(CKB)37111014700041(DE-He213)978-981-97-6851-6(EXLCZ)993711101470004120241230d2024 u| 0engurcnu||||||||txtrdacontentcrdamediacrrdacarrierAntibiotics and Their Mechanisms of Action /edited by Deepesh Nagarajan1st ed. 2024.Singapore :Springer Nature Singapore :Imprint: Springer,2024.1 online resource (185 pages)9789819768509 9819768500 Chapter 1. The history of antibiotics -- Chapter 2. Methods for antibiotic discovery -- Chapter 3. Penicillin -- Chapter 4. Vancomycin -- Chapter 5. Antimicrobial peptides -- Chapter 6. Quinolones -- Chapter 7. Rifampicin -- Chapter 8. Tetracyclines -- Chapter 9. Streptomycin -- Chapter 10. Chloramphenicol -- Chapter 11. Antimetabolites -- Chapter 12. Mycolic acid inhibitors -- Chapter 13. Other antimicrobial agents -- Chapter 14. Antibiotic Pharmacology and Biopharmaceutics.“Antibiotics and their mechanisms of action” provides a deep and lucid understanding of the mechanisms of action of various antibiotics. The experiments followed to decipher the mechanisms have been explained in great depth, which is a novelty in this textbook. Most undergraduate textbooks on antibiotics place emphasis on their use from the clinician’s perspective. Taking penicillin as an example, typical textbooks discuss its discovery, penicillin’s β-lactam derivatives, routes of administration, the diseases and pathogens treated with β-lactam antibiotics, side effects, and finally the mechanisms of resistance that have evolved against penicillin. No description of how its mechanism of action was deciphered is ever provided. This textbook describes how simple experiments using protoplasts, covalent affinity chromatography, SYPRO Orange binding assays, and X-ray crystallography have all been used to elucidate the mechanism of action of penicillin. Like this, the complicated mechanisms of action of different antibiotics have been presented in simple terms with the help of original illustrations. Chapters 8-10 cover the mechanisms of action of anti-ribosomal antibiotics (tetracycline, streptomycin, chloramphenicol). Elucidating the mechanisms of action for these antibiotics requires complex experiments such as ex vivo transcription via a cell-free ribosomal reaction mixture, photolabelling experiments, and sucrose density gradient centrifugation of radiolabelled translation reactants and products. These experiments are conveyed to the reader in the simplest possible language and with easily understandable, appealing illustrations. Finally, the reader is challenged at the end of every chapter with original questions, testing whether hecan apply the concepts he learned in the preceding chapter to research-level problems. This book is written primarily for undergraduate students in microbiology or biotechnology courses to help them develop a simplistic yet comprehensive understanding of the mechanisms underlying antibiotics action. It is believed that this book will prepare the students for PhD level research.PharmacologyMedical microbiologyDrug delivery systemsPharmacologyMedical MicrobiologyDrug DeliveryAntibiòticsthubMecanismes de reacció (Química)thubLlibres electrònicsthubPharmacology.Medical microbiology.Drug delivery systems.Pharmacology.Medical Microbiology.Drug Delivery.AntibiòticsMecanismes de reacció (Química)615Nagarajan Deepesh1781745MiAaPQMiAaPQMiAaPQBOOK9910920447403321Antibiotics and Their Mechanisms of Action4306566UNINA