04111nam 22005774a 450 991087788440332120200520144314.01-280-28688-197866102868810-470-32559-30-471-73949-90-471-73948-0(CKB)1000000000355062(EBL)242871(OCoLC)85784717(SSID)ssj0000165610(PQKBManifestationID)11180866(PQKBTitleCode)TC0000165610(PQKBWorkID)10162668(PQKB)10885562(MiAaPQ)EBC242871(EXLCZ)99100000000035506220041213d2006 uy 0engur|n|---|||||txtccrGreen fluorescent protein properties, applications, and protocols /edited by Martin Chalfie, Steven R. Kain2nd ed.Hoboken, N.J. Wiley-Intersciencec20061 online resource (486 p.)Methods of biochemical analysis ;v. 47Description based upon print version of record.0-471-73682-1 Includes bibliographical references and index.Discovery of green fluorescent protein / Osamu Shimomura -- Photons for reporting molecular events: green fluorescent protein and four luciferase systems / J. Woodland Hastings and James G. Morin -- Biochemical and physical properties of green fluorescent protein / William W. Ward -- The three-dimensional structure of green fluorescent protein and its implications for function and design / George N. Phillips, Jr -- Molecular biology and mutation of green fluorescent protein / David A. Zacharias, Roger Y. Tsien -- Discovery and properties of GFP-like proteins from nonbioluminescent anthozoa / Konstantin A. Lukyanov ... [et al.] -- Evolution of function and color in GFP-like proteins / Mikhail V. Matz, Yulii A. Labas, and Juan Ugalde -- The uses of green fluorescent protein in prokaryotes / Raphael H. Valdivia, Brendan P. Cormack, and Stanley Falkow -- The uses of green fluorescent protein in yeasts / Amy L. Hitchcock, Jason A. Kahana, and Pamela A. Silver -- Uses of GFP in Caenorhabditis elegans / Oliver Hobert and Paula Loria -- Green fluorescent protein applications in drosophila / Tulle Hazelrigg and Jennifer H. Mansfield -- The uses of green fluorescent protein in plants / Jim Haseloff and Kirby R. Siemering -- Uses of GFP in transgenic vertebrates / Sean Magason ... [et al.] -- The uses of green fluorescent protein in mammalian cells / Theresa H. Ward and Jennifer Lippincott-Schwartz -- Practical considerations for use of reef coral fluorescent proteins in mammalian cells: applications in fluorescent microscopy and flow cytometry / Yu Fang ... [et al.] -- Pharmaceutical applications of GFP and RCFP / Nicola Bevan and Stephen Rees -- Reassembled GFP: detecting protein-protein interactions and protein expression patterns / Thomas J. Magliery and Lynne Regan.Since the discovery of the gene for green fluorescent protein (GFP), derived from jellyfish, this protein that emits a green glow has initiated a revolution in molecular biosciences. With this tool, it is now possible to visualize nearly any protein of interest in any cell or tissue of any species. Since the publication of the first edition, there have been tremendously significant technological advances, including development of new mutant variants. Proteins are now available in yellow and blue, and Novel Fluorescent Proteins (NFPs) have expanded their utility in developing biosensors, biologMethods of biochemical analysis ;v. 47.Green fluorescent proteinLaboratory manualsGreen fluorescent protein572/.6Chalfie Martin1758104Kain Steven1758105MiAaPQMiAaPQMiAaPQBOOK9910877884403321Green fluorescent protein4196225UNINA