03003nam 2200577Ia 450 991083097160332120230607213121.01-280-36659-197866103665900-470-24459-30-471-46103-20-471-22390-5(CKB)111056485581914(EBL)152099(OCoLC)475871949(SSID)ssj0000204810(PQKBManifestationID)11168594(PQKBTitleCode)TC0000204810(PQKBWorkID)10192119(PQKB)11661517(MiAaPQ)EBC152099(EXLCZ)9911105648558191420010214d2001 uy 0engur|n|---|||||txtccrMolecular biology problem solver[electronic resource] a laboratory guide /edited by Alan S. GersteinNew York Wileyc20011 online resource (591 p.)Description based upon print version of record.0-471-37972-7 Includes bibliographical references and index.MOLECULAR BIOLOGY PROBLEM SOLVER; Contents; Preface; Contributors; Chapter 1. Preparing for Success in the Laboratory; Chapter 2. Getting What You Need from a Supplier; Chapter 3. The Preparation of Buffers and Other Solutions: A Chemist's Perspective; Chapter 4. How to Properly Use and Maintain Laboratory Equipment; Chapter 5. Working Safely with Biological Samples; Chapter 6. Working Safely with Radioactive Materials; Chapter 7. DNA Purification; Chapter 8. RNA Purification; Chapter 9. Restriction Endonucleases; Chapter 10. Nucleotides, Oligonucleotides, and Polynucleotides; Chapter 11. PCRChapter 12. ElectrophoresisChapter 13. Western Blotting; Chapter 14. Nucleic Acid Hybridization; Chapter 15. E. coli Expression Systems; Chapter 16. Eukaryotic Expression; IndexMost research in the life sciences involves a core set of molecular-based equipment and methods, for which there is no shortage of step-by-step protocols. Nonetheless, there remains an exceedingly high number of inquiries placed to commercial technical support groups, especially regarding problems. Molecular Biology Problem Solver: A Laboratory Guide asks the reader to consider crucial questions, such as:Have you selected the most appropriate research strategy?Have you identified the issues critical to your successful application of a technique?Are you familiar Molecular biologyMethodologyMolecular biologyLaboratory manualsMolecular biologyMethodology.Molecular biology572.8572.8078Gerstein Alan S.1957-1675337MiAaPQMiAaPQMiAaPQBOOK9910830971603321Molecular biology problem solver4040714UNINA