05445nam 2200673Ia 450 991083074460332120170810182601.01-119-99027-01-283-37396-397866133739600-470-97913-50-470-97912-7(CKB)3400000000000314(EBL)661780(OCoLC)705353428(SSID)ssj0000477073(PQKBManifestationID)11320049(PQKBTitleCode)TC0000477073(PQKBWorkID)10480622(PQKB)11539724(MiAaPQ)EBC661780(PPN)243264615(EXLCZ)99340000000000031420101004d2011 uy 0engur|n|---|||||txtccrLabel-free technologies for drug discovery[electronic resource] /editors, Matthew Cooper, Lorenz MayrChichester, West Sussex, U.K. John Wiley & Sons20111 online resource (350 p.)Description based upon print version of record.0-470-74683-1 Includes bibliographical references and index.Label-Free TechnologiesFor Drug Discovery; Contents; Preface; List of Contributors; 1 The Revolution of Real-Time, Label-Free Biosensor Applications; 1.1 Introduction; 1.2 SPR Pessimists; 1.3 Setting Up Experiments; 1.4 Data Processing and Analysis; 1.5 The Good News; References; 2 Design and Implementation of Vertically Emitting Distributed Feedback Lasers for Biological Sensing; 2.1 Introduction; 2.2 DFB Laser Biosensor Design; 2.3 Fabrication and Instrumentation; 2.4 Experimental Results; 2.4.1 Vertically Emitting DFB Laser; 2.4.2 Bulk Material Sensing; 2.4.3 Sensitivity Resolution2.4.4 Small Molecule Binding Detection2.5 Conclusions; Acknowledgements; References; 3 SPR Screening of Chemical Microarrays for Fragment-Based Discovery; 3.1 Introduction; 3.2 Key Features of Fragment Screening; 3.3 SPR Fragment Screening; 3.4 Synthesis of Library Compounds; 3.5 Library Design and Array Content; 3.6 Chemical Microarray Production; 3.7 Surface Plasmon Resonance; 3.8 SPR Imaging; 3.9 Array Visualization and Analysis; 3.10 Follow-up; 3.11 Applications: MMP Case Study; 3.11.1 Search for New Binding Modes; 3.11.2 Selectivity Studies; 3.12 Other Target Classes; 3.13 ConclusionReferences4 The CellKey R System: A Label-Free Cell-Based Assay Platform for Early Drug Discovery Applications; 4.1 Introduction; 4.2 Cellular Impedance Technology; 4.3 Target Identification and Validation; 4.4 Screening and Lead Optimization; 4.5 Conclusion; References; 5 Dynamic and Label-Free Cell-Based Assays Using the xCELLigence System; 5.1 Introduction; 5.2 The xCELLigence System; 5.3 Principle of Detection; 5.4 Applications; 5.4.1 Cell Proliferation, Cytotoxicity and Time Dependent Cellular Response Profiling; 5.5 Functional Assays for G-Protein Coupled Receptors; 5.6 ConclusionReferences6 Selecting the Best HTS Hits to Move Forward: ITC Ligand Binding Characterization Provides Guidance; 6.1 Introduction; 6.2 Principles of Isothermal Titration Calorimetry (ITC); 6.3 Applications of ITC in Hit Validation; 6.3.1 Assay Design for Hit Confirmation and Affinity Determination; 6.3.2 Identification of Nonspecific Binders, Unstable Protein Targets and Multiple Binding Sites; 6.3.3 High Speed ITC Hit Characterization Assays; 6.4 Applications of ITC in Fragment-Based Drug Discovery; 6.4.1 Measuring Weak Affinities by ITC; 6.5 Applications of ITC in Mechanism of Action Studies6.6 Applications of ITC in Lead Optimization6.7 ITC as an Enzyme Activity Monitor; 6.8 Conclusion; References; 7 Incorporating Transmitted Light Modalities into High-Content Analysis Assays; 7.1 Introduction; 7.2 Transmitted Light (Bright Field) Imaging; 7.3 Image Analysis of Phase Contrast Images; 7.4 Conclusion; References; 8 Nonradioactive Rubidium Efflux Assay Technology for Screening of Ion Channels; 8.1 Introduction; 8.2 Ion Channels as Drug Targets; 8.3 Ion Channel Assays and Screening; 8.4 Nonradioactive Rubidium Efflux Assay Based on Atomic Absorption Spectrometry8.5 A Typical Assay ProtocolOver the past two decades the benefits of label-free biosensor analysis have begun to make an impact in the market, and systems are beginning to be used as mainstream research tools in many drug discovery laboratories. Label-Free Technologies For Drug Discovery summarises the latest and emerging developments in label-free detection systems, their underlying technology principles and end-user case studies that reveal the power and limitations of label-free in all areas of drug discovery. Label-free technologies discussed include SPR, NMR, high-throughput mass spectrometry, resonanDrug developmentBiosensorsPharmaceutical biotechnologyDrug development.Biosensors.Pharmaceutical biotechnology.615.19615/.19Cooper M. A(Matthew A.)1658862Mayr Lorenz1658863MiAaPQMiAaPQMiAaPQBOOK9910830744603321Label-free technologies for drug discovery4013152UNINA