05281nam 2200661Ia 450 991083071360332120230617031401.01-280-27145-00-470-86217-397866102714500-470-46889-00-470-86729-9(CKB)1000000000019112(EBL)158129(OCoLC)53813738(SSID)ssj0000192870(PQKBManifestationID)11166250(PQKBTitleCode)TC0000192870(PQKBWorkID)10215595(PQKB)11582930(MiAaPQ)EBC158129(EXLCZ)99100000000001911220020814d2003 uy 0engur|n|---|||||txtccrLiquid chromatography-mass spectrometry[electronic resource] an introduction /Robert E. ArdreyNew York J. Wiley20031 online resource (298 p.)Analytical techniques in the sciencesDescription based upon print version of record.0-471-49801-7 0-471-49799-1 Includes bibliographical references and index.LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY: AN INTRODUCTION; Contents; Series Preface; Preface; Acknowledgements; Abbreviations, Acronyms and Symbols; About the Author; 1 Introduction; 1.1 What are the Advantages of Linking High Performance Liquid Chromatography with Mass Spectrometry?; 1.2 What Capabilities are Required of the Combination?; 1.3 What Problems, if Any, Have to be Addressed to Allow the LC-MS Combination to Function, and Function Effectively?; References; 2 Liquid Chromatography; 2.1 Introduction; 2.2 High Performance Liquid Chromatography; 2.2.1 Pump2.2.2 Sample Introduction (Injector)2.2.3 Mobile Phase; 2.2.4 Stationary Phase; 2.2.5 Detectors; 2.3 Chromatographic Properties; 2.4 Identification Using High Performance Liquid Chromatography; 2.5 Quantitation Using High Performance Liquid Chromatography; 2.6 The Need for High Performance Liquid Chromatography-Mass Spectrometry; References; 3 Mass Spectrometry; 3.1 Introduction; 3.2 Ionization Methods; 3.2.1 Electron Ionization; 3.2.2 Chemical Ionization; 3.2.3 Fast-Atom Bombardment; 3.2.4 Matrix-Assisted Laser Desorption Ionization; 3.2.5 Negative Ionization; 3.3 Ion Separation3.3.1 The Quadrupole Mass Analyser3.3.2 The (Quadrupole) Ion-Trap Mass Analyser; 3.3.3 The Double-Focusing and Tri-Sector Mass Analysers; 3.3.4 The Time-of-Flight Mass Analyser; 3.4 Tandem Mass Spectrometry (MS-MS); 3.4.1 Instrumentation; 3.4.2 Techniques; 3.5 Data Acquisition; 3.5.1 Identification; 3.5.2 Quantitation; 3.6 Processing of Mass Spectral Data; 3.6.1 The Total-Ion-Current Trace; 3.6.2 Qualitative Analysis; 3.6.3 Quantitative Analysis; 3.6.4 The Use of Tandem Mass Spectrometry; References; 4 Interface Technology; 4.1 Introduction; 4.2 The Moving-Belt Interface4.3 The Direct-Liquid-Introduction Interface4.4 The Continuous-Flow/Frit (Dynamic) Fast-Atom-Bombardment Interface; 4.5 The Particle-Beam Interface; 4.6 The Thermospray Interface; 4.7 The Electrospray Interface; 4.7.1 The Mechanism of Electrospray Ionization; 4.7.2 Sample Types; 4.7.3 The Appearance of the Electrospray Spectrum; 4.7.4 Structural Information from Electrospray Ionization; 4.8 The Atmospheric-Pressure Chemical Ionization Interface; 4.8.1 The Mechanism of Atmospheric-Pressure Chemical Ionization; References5 Applications of High Performance Liquid Chromatography-Mass Spectrometry5.1 Method Development; 5.1.1 The Use of Experimental Design for Method Development; 5.1.2 The Choice of Electrospray or APCI; 5.2 The Molecular Weight Determination of Biopolymers; 5.2.1 Electrospray Spectra of Co-Eluting Components; 5.2.2 The Use of Selected-Ion Monitoring to Examine the Number of Terminal Galactose Moieties on a Glycoprotein; 5.2.3 The Effect of Mobile-Phase Additives and Cone-Voltage; 5.3 Structure Determination of Biopolymers; 5.3.1 Amino Acid Sequencing of Proteins5.3.2 The Use of Enzymes for Amino Acid SequencingFirst explaining the basic principles of liquid chromatography and mass spectrometry and then discussing the current applications and practical benefits of LC-MS, along with descriptions of the basic instrumentation, this title will prove to be the indispensable reference source for everyone wishing to use this increasingly important tandem technique.* First book to concentrate on principles of LC-MS* Explains principles of mass spectrometry and chromatography before moving on to LC-MS* Describes instrumental aspects of LC-MS* Discusses current applications of LC-MS and shows bAnalytical techniques in the sciences.Liquid chromatographyMass spectrometryLiquid chromatography.Mass spectrometry.543.84543/.0894Ardrey R. E1606744MiAaPQMiAaPQMiAaPQBOOK9910830713603321Liquid chromatography-mass spectrometry3932687UNINA