04860nam 2200613Ia 450 991083070160332120170918181121.01-282-46084-697866124608453-527-62665-43-527-62664-6(CKB)1000000000820644(EBL)481899(OCoLC)566109955(SSID)ssj0000342913(PQKBManifestationID)11252510(PQKBTitleCode)TC0000342913(PQKBWorkID)10288879(PQKB)10955362(MiAaPQ)EBC481899(PPN)198274629(EXLCZ)99100000000082064420071213d2009 uy 0engur|n|---|||||txtccrSingle cell analysis[electronic resource] technologies and applications /edited by Dario AnselmettiWeinheim Wiley-VCHc20091 online resource (286 p.)Description based upon print version of record.3-527-31864-X Includes bibliographical references and index.Single Cell Analysis: Technologies and Applications; Contents; Foreword; Preface; List of Contributors; Part I Single Cell Analysis: Imaging; 1 Single Molecule Fluorescence Monitoring in Eukaryotic Cells: Intranuclear Dynamics of Splicing Factors; 1.1 Motivation; 1.2 Experimental Approach; 1.3 Single Particle Tracking within Living Cells; 1.4 Pre-Messenger RNA Splicing; 1.5 Intranuclear Splicing Factor Tracking; 1.6 Intranuclear U1 snRNP Splicing Factor Binding; 1.7 Events in Speckles; 1.8 Intranuclear U1 snRNP Mobility; 1.9 Perspectives of Single Molecule Microscopy; References2 Gene Classification and Quantitative Analysis of Gene Regulation in Bacteria using Single Cell Atomic Force Microscopy and Single Molecule Force Spectroscopy2.1 Introduction; 2.2 AFM on Paracrystalline Cell Surface Layers of C. glutamicum: Protein Sequence Information and Morphology; 2.3 Imaging of Living C. glutamicum Cells with Molecular Resolution: Genes, Transcriptional Regulation and Morphology; 2.4 Single Molecule Force Spectroscopy on Specific Protein-DNA Complexes: Transcriptional Regulation in S. meliloti2.5 Effector-Induced Protein-DNA Binding on the Single Molecule Level: Quorum Sensing in S. meliloti2.6 Conclusion; References; 3 Cellular Cryo-Electron Tomography (CET): Towards a Voyage to the Inner Space of Cells; 3.1 Introduction; 3.2 Tomography with the Electron Microscope - a Practical Perspective; 3.2.1 Sample Preparation; 3.2.2 Instrumental and Technical Requirements; 3.2.3 Alignment, Reconstruction and Visualization; 3.3 Molecular Interpretation of Cellular Tomograms; 3.4 Outlook: The Future is Bright; References; Part II Single Cell Analysis: Technologies; 4 Single Cell Proteomics4.1 Introduction4.2 The Challenge; 4.3 Single Cell Proteomics: Mass Spectrometry; 4.4 Single Cell Separations; 4.5 Ultrasensitive Protein Analysis: Capillary Electrophoresis with Laser-Induced Fluorescence Detection; 4.6 Capillary Sieving Electrophoresis of Proteins from a Single Cancer Cell; 4.7 Cell Cycle-dependent Single Cell Capillary Sieving Electrophoresis; 4.8 Tentative Identification of Proteins in Single Cell Electropherograms; 4.9 Capillary Micellar and Submicellar Separation of Proteins from a Single Cell; 4.10 Two-Dimensional Capillary Electrophoresis of Proteins in a Single Cell4.11 Single Copy Detection of Specific Proteins in Single Cells4.12 Conclusion; References; 5 Protein Analysis of Single Cells in Microfluidic Format; 5.1 Introduction; 5.2 Microfluidic Single Cell Analysis Concept; 5.2.1 Single Cell Selection and Trapping; 5.2.2 Single Cell Lysis; 5.3 Single Cell Electrophoretic Separation and Detection of Proteins; 5.3.1 Label-Based Fluorescence Detection; 5.3.2 Label-Free Fluorescence Detection; 5.3.2.1 UV-LIF in Quartz Microfluidic Devices; 5.3.2.2 UV-LIF in PDMS Microfluidic Devices; 5.3.2.3 Single Cell UV-LIF Electrophoretic Analysis5.4 Future Directions in Single Cell AnalysisThe first-ever comprehensive overview of the methods used in this key technology in modern biology provides the latest working knowledge needed by every scientist entering this growing field. It covers all the current technology and application areas, from microscopy and spectroscopy to proteomics and microfluidics.CellsAnalysisCytologyCellsAnalysis.Cytology.571.6Anselmetti D1640802MiAaPQMiAaPQMiAaPQBOOK9910830701603321Single cell analysis3984512UNINA