05441nam 2200685Ia 450 991082998700332120230829010339.01-280-72271-197866107227163-527-60914-83-527-60869-9(CKB)1000000000375863(EBL)481587(OCoLC)78210231(SSID)ssj0000221275(PQKBManifestationID)11190872(PQKBTitleCode)TC0000221275(PQKBWorkID)10161868(PQKB)11553397(MiAaPQ)EBC481587(EXLCZ)99100000000037586320050207d2006 uy 0engur|n|---|||||txtccrPhotoproteins in bioanalysis[electronic resource] /edited by Sylvia Daunert and Sapna K. DeoWeinheim Wiley-VCHc20061 online resource (258 p.)Description based upon print version of record.3-527-31016-9 Includes bibliographical references and index.Photoproteins in Bioanalysis; Contents; Preface; List of Contributors; 1 The Photoproteins; 1.1 Discovery of Photoprotein; 1.2 Various Types of Photoproteins Presently Known; 1.2.1 Radiolarian (Protozoa) Photoproteins; 1.2.2 Coelenterate Photoproteins; 1.2.3 Ctenophore Photoproteins; 1.2.4 Pholasin (Pholas Luciferin); 1.2.5 Chaetopterus Photoprotein; 1.2.6 Polynoidin; 1.2.7 Symplectin; 1.2.8 Luminodesmus Photoprotein; 1.2.9 Ophiopsila Photoprotein; 1.3 Basic Strategy of Extracting and Purifying Photoproteins; 1.4 The Photoprotein Aequorin; 1.4.1 Extraction and Purification of Aequorin1.4.1.1 Hydrophobic Interaction Chromatography1.4.2 Properties of Aequorin; 1.4.2.1 Stability; 1.4.2.2 Freeze-drying; 1.4.3 Specificity to Ca(2+); 1.4.4 Luminescence of Aequorin by Substances Other Than Divalent Cations; 1.4.5 Mechanism of Aequorin Luminescence and Regeneration of Aequorin; 1.4.5.1 Structure of Aequorin; 1.4.5.2 Luminescence Reaction; 1.4.5.3 Regeneration; 1.4.6 Inhibitors of Aequorin Luminescence; 1.4.7 Recombinant Aequorin; 1.4.8 Semi-synthetic Aequorins; 1.4.8.1 e-Aequorins; References; 2 Luminous Marine Organisms; 2.1 Introduction; 2.1.1 Non-luminous Taxa2.1.2 Luminous Taxa2.2 Taxonomic Distribution of Bioluminescence; 2.2.1 Bacterial Luminescence; 2.2.2 Dinoflagellate Luciferin; 2.2.3 Cypridina (Vargula) Luciferin; 2.2.4 Coelenterazine; 2.2.5 Other Luciferins: Known and Unknown; 2.3 Functions; 2.3.1 Startle or Distract; 2.3.2 Burglar Alarm; 2.3.3 Counterillumination; 2.3.4 Mating Displays; 2.3.5 Prey Attraction; References; 3 Beetle Luciferases: Colorful Lights on Biological Processes and Diseases; 3.1 Introduction; 3.2 Beetle Luciferases; 3.3 Bioanalytical Assays of ATP; 3.3.1 Biomass Estimation and Microbiological Contamination3.3.2 Cytotoxicity and Cell Viability Tests3.3.3 Enzymatic Assays; 3.4 Luciferases as Reporter Genes; 3.4.1 Dual and Multiple Reporter Assays; 3.5 Biophotonic Imaging in Animals: A Living Light on Diseases; 3.5.1 Pathogen Infection in Living Models; 3.5.2 Drug Screening; 3.5.3 Tumor Proliferation and Regression Studies; 3.5.4 Gene Delivery and Gene Therapy; 3.5.5 Luciferase as Biomarkers for Cell Trafficking Studies; 3.5.6 Immunoassays; 3.6 Biophotonic Imaging in Plants; 3.7 Biosensors: Sensing the Environment; 3.8 Novel Luciferases: Different Colors for Different Occasions; References4 Split Luciferase Systems for Detecting Protein-Protein Interactions in Mammalian Cells Based on Protein Splicing and Protein Complementation4.1 Introduction; 4.2 Protein Splicing-based Split Firefly Luciferase System [23]; 4.2.1 Split Luciferase Works as a Probe for Protein Interaction; 4.3 Split Renilla Luciferase Complementation System [33]; 4.3.1 Time Course of the Interaction Between Y941 and SH2n; 4.3.2 Location of the Interaction Between Y941 and SH2n; References; 5 Photoproteins in Nucleic Acid Analysis; 5.1 Hybridization Assays; 5.2 Quantitative Polymerase Chain Reaction5.3 Genotyping of Single-nucleotide PolymorphismsThe use of light-emitting proteins for the detection of biomolecules provides fast and sensitive methods which overcome the disadvantages of radioactive labels and the high cost of fluorescent dyes.This reference work summarizes modern advanced techniques and their applications and includes practical examples of assays based on photoproteins. The book presents contemporary key topics like luminescent marine organisms, DNA probes, reporter gene assays and photoproteins, ratiometric sensing, use of photoproteins for in vivo functional imaging and luminescent proteins in binding assays, to naProteinsBiomoleculesAnalysisBiochemistryTechniquePhotoluminescenceBioluminescenceProteins.BiomoleculesAnalysis.BiochemistryTechnique.Photoluminescence.Bioluminescence.572.435Daunert Sylvia1659712Deo Sapna K1659713MiAaPQMiAaPQMiAaPQBOOK9910829987003321Photoproteins in bioanalysis4014488UNINA