05336nam 2200649 a 450 991082986100332120230721033029.01-281-83132-897866118313250-470-36963-90-470-36983-3(CKB)1000000000550532(EBL)366718(OCoLC)437234346(SSID)ssj0000222367(PQKBManifestationID)11215760(PQKBTitleCode)TC0000222367(PQKBWorkID)10170975(PQKB)10135299(MiAaPQ)EBC366718(EXLCZ)99100000000055053220071217d2008 uy 0engur|n|---|||||txtccrPlant proteomics[electronic resource] technologies, strategies, and applications /edited by Ganesh Kumar Agrawal, Randeep RakwalHoboken, N.J. J. Wileyc20081 online resource (818 p.)Wiley-Interscience series in mass spectrometryDescription based upon print version of record.0-470-06976-7 Includes bibliographical references and index.PLANT PROTEOMICS; CONTENTS; PREFACE; CONTRIBUTORS; ACRONYMS AND ABBREVIATIONS; 1 AN INTRODUCTION TO PROTEOMICS: APPLICATIONS TO PLANT BIOLOGY; 1.1 Proteomics Defined; 1.2 Proteomics Applied; References; PART I TECHNOLOGIES; 2 GEL-BASED PROTEOMICS; 2.1 Introduction and Brief Bibliographic Review; 2.2 SDS-PAGE; 2.3 IEF; 2.4 2D Maps; 2.5 Conclusions; 2.6 Five-Year Viewpoint; References; 3 MASS SPECTROMETRY-BASED PROTEOMICS: IDENTIFYING PLANT PROTEINS; 3.1 Introduction and Brief Bibliographic Review; 3.2 Instrumentation; 3.3 MALDI; 3.4 ESI; 3.5 Mass Analyzers; 3.6 Ion Detectors3.7 Sample Preparation3.8 Protein Identification; 3.9 Conclusions; 3.10 Five-Year Viewpoint; References; 4 CHEMICAL PROTEOMICS; 4.1 Introduction; 4.2 Strategies For Activity-Based Protein Profiling (ABPP); 4.3 Case Study: Development of Molecular Tools Targeting Plant Kinases; 4.4 Conclusions; 4.5 Five-Year Viewpoint; References; 5 THE ARABIDOPSIS LOCALIZOME: SUBCELLULAR PROTEIN LOCALIZATION AND INTERACTIONS IN ARABIDOPSIS; 5.1 Protein Compartmentalization in Plant Cells; 5.2 Experimental Determination of Protein Localization5.3 In Vivo Imaging Approaches to Protein Localization and Interaction5.4 Plant Cell Cultures for Studying Protein Localization; 5.5 Protein-Protein Interaction In Vivo: FRET; 5.6 Perspectives: Integrating Predictive and Experimental Protein Localization Data; References; 6 SECRETOME: TOWARD DECIPHERING THE SECRETORY PATHWAYS AND BEYOND; 6.1 Introduction and Brief Bibliographic Review; 6.2 Methodology and Strategy; 6.3 A Case Study: In Planta and In Vitro Protein Profiles of Soluble and Secreted Proteins in Rice; 6.4 Conclusions; 6.5 Five-Year Viewpoint; References; 7 PEPTIDOMICS7.1 Introduction and Brief Bibliographic Review7.2 Separation Technology; 7.3 MS Technology; 7.4 Bioinformatics and Data Mining; 7.5 Differential Peptide Display; 7.6 ID LC-MALDI; 7.7 2D CA-RP-LC-ESI-MS; 7.8 Applications; 7.9 Peptides and Proteases; 7.10 Conclusions; 7.11 Five-Year Viewpoint; References; PART II COMPUTATIONAL PROTEOMICS; 8 BIOINFORMATICS IN GEL-BASED PROTEOMICS; 8.1 Introduction and Brief Bibliographic Review; 8.2 Methodology and Strategy; 8.3 Experimental Results and Applications; 8.4 Conclusions; 8.5 Five-Year Viewpoint; References; 9 BIOINFORMATICS IN MS-BASED PROTEOMICS9.1 Introduction9.2 Database Searching; 9.3 Peptide De Novo Sequencing; 9.4 Conclusions and Five-Year Viewpoint; References; PART III EXPRESSION PROTEOMICS; 10 AN OVERVIEW OF THE ARABIDOPSIS PROTEOME; 10.1 Introduction and Brief Bibliographic Review; 10.2 Methodology and Strategy; 10.3 Experimental Results and Applications; 10.4 Conclusions; 10.5 Five-Year Viewpoint; References; 11 RICE PROTEOME AT A GLANCE; 11.1 Introduction and Brief Bibliographic Review; 11.2 Methodology and Strategy; 11.3 Experimental Results and Applications; 11.4 Conclusions; 11.5 Five-Year Viewpoint; References12 PROTEOMICS OF LEGUME PLANTSConfidently face the challenges of proteomics research specific to plant science with the information in Plant Proteomics, which will introduce you to the techniques and methodologies required for the study of representative plant species. Read about proteomics studies in Arabidopsis, rice, and legumes and find information about common technologies like mass spectrometry and gel electrophoresis. Discover expression proteomics, functional proteomics, structural proteomics, bioinformatics, and systems biology, understand how to conduct proteomics studies in developing countries and underfWiley-Interscience series on mass spectrometry.Plant proteinsPlant proteomicsPlant proteins.Plant proteomics.572572.62Agrawal Ganesh Kumar1603701Rakwal Randeep1603702MiAaPQMiAaPQMiAaPQBOOK9910829861003321Plant proteomics3928191UNINA