05678nam 2200721Ia 450 991082909340332120200520144314.01-283-33226-497866133322641-118-13538-51-118-13539-31-118-13536-9(CKB)2550000000064527(EBL)818454(OCoLC)768230312(SSID)ssj0000538448(PQKBManifestationID)11331452(PQKBTitleCode)TC0000538448(PQKBWorkID)10559164(PQKB)10142768(MiAaPQ)EBC818454(Au-PeEL)EBL818454(CaPaEBR)ebr10510539(CaONFJC)MIL333226(EXLCZ)99255000000006452720110611d2012 uy 0engur|n|---|||||txtccrLC-NMR and other hyphenated NMR techniques overview and applications /Maria Victoria Silva Elipe1st ed.Hoboken, NJ Wileyc20121 online resource (240 p.)THEi Wiley ebooksDescription based upon print version of record.0-470-54834-7 Includes bibliographical references and index.LC-NMR and Other Hyphenated NMR Techniques: Overview and Applications; Contents; Preface; Abbreviations, Symbols, and Units; 1. Basic Concepts of NMR Spectroscopy; 1.1 Introduction; 1.2 Basic Knowledge Regarding the Physics of NMR Spectroscopy; 1.3 Basic Parameters for NMR Interpretation; 1.3.1 Chemical Shift; 1.3.2 Spin-Spin Coupling Constants; 1.3.3 Spin Systems; 1.3.4 Signal Intensities; 1.3.5 Bond Correlations; 1.3.6 Spatial Correlations; 1.3.7 Other Topics; 1.4 Conclusions; References; 2. Historical Development of NMR and LC-NMR; 2.1 Introduction; 2.2 Historical Development of NMR2.3 Historical Development of LC-NMR2.4 Historical Development of Other Analytical Techniques Hyphenated with NMR; 2.5 Current Trends; References; 3. Basic Technical Aspects and Operation of LC-NMR and LC-MS-NMR; 3.1 Introduction; 3.2 Technical Considerations Regarding LC-NMR; 3.2.1 Solvent Compatibility; 3.2.2 Solvent Suppression; 3.2.3 NMR Flow Cell; 3.2.4 LC-NMR Sensitivity; 3.3 Technical Considerations Regarding LC-MS-NMR; 3.3.1 Deuterated Solvents; 3.4 Modes of Operation of LC-NMR; 3.4.1 On-Flow Mode; 3.4.2 Stop-Flow Mode; 3.4.3 Time-Sliced Mode; 3.4.4 Loop Collection Mode3.5 Modes of Operation of LC-MS-NMR3.5.1 On-Flow Mode; 3.5.2 Stop-Flow Mode; 3.6 Other Modes of Operation; 3.7 Challenging Considerations; 3.7.1 Air Bubbles; 3.7.2 Carryover with and Without an Autosampler; 3.7.3 Sample Solubility and Precipitation; 3.7.4 Flow Cell and System Cleaning; 3.7.5 Flow Rate and Magnetic Susceptibility; 3.7.6 Quantitation; 3.8 Conclusions; References; 4. Applications of LC-NMR; 4.1 Introduction; 4.2 Applications of LC-NMR; 4.2.1 Natural Products; 4.2.2 Drug Metabolism; 4.2.3 Drug Discovery; 4.2.4 Impurity Characterization; 4.2.5 Degradation Products4.2.6 Food Analysis4.2.7 Polymers; 4.2.8 Metabolomics and Metabonomics; 4.2.9 Isomers, Tautomers, and Chiral Compounds; 4.2.10 Others Areas; 4.3 Conclusions and Future Trends; References; 5. Applications of LC-MS-NMR; 5.1 Introduction; 5.2 Applications of LC-MS-NMR; 5.2.1 Natural Products; 5.2.2 Drug Metabolism; 5.2.3 Drug Discovery and Development; 5.2.4 Metabolomics and Metabonomics; 5.2.5 Others Areas; 5.3 Conclusions and Future Trends; References; 6. Hyphenation of NMR with Other Analytical Separation Techniques; 6.1 Introduction; 6.2 GC-NMR; 6.3 GPC-NMR; 6.4 SEC-NMR; 6.5 SFC-NMR6.6 SFE-NMR6.7 CE-NMR; 6.8 CEC-NMR; 6.9 CZE-NMR; 6.10 cITP-NMR; 6.11 CapLC-NMR; 6.12 SPE-NMR; 6.13 SPE-MS-NMR; 6.14 Conclusions and Future Trends; References; 7. Special Topics and Applications Related to LC-NMR; 7.1 Introduction; 7.2 Off-Line Versus Online NMR for Structural Elucidation; 7.2.1 Cases Solved Off-Line; 7.2.2 Cases Solved Online; 7.3 Analysis of Chiral Molecules by NMR; 7.3.1 Classical Approach: Off-Line; 7.3.2 Nonclassical Approach: Online; 7.4 Monitoring Chemical Reactions In Situ; 7.4.1 Classical Approach: Off-Line; 7.4.2 Nonclassical Approach: Online7.5 Analysis of Mixtures Off-Line, Online, and by Other NMR MethodologiesThis practical guide provides a basic overview of the pros and cons of NMR spectroscopy as both a hyphenated and non-hyphenated technique. The book begins with a description of basic NMR concepts for the structural elucidation of organic compounds and then details the historical development of NMR and hyphenated NMR in the structural elucidation world, followed by applications of hyphenated NMR as LC-NMR and LC-MS-NMR in industry and academia. It also contains updated information on the latest advancements and applications of LC-NMR in such areas as degradation products, drug metabolism, foodTHEi Wiley ebooks.Nuclear magnetic resonance spectroscopyIndustrial applicationsOrganic compoundsAnalysisDrug developmentNuclear magnetic resonance spectroscopyIndustrial applications.Organic compoundsAnalysis.Drug development.543/.66SCI078000bisacshSilva Elipe Maria V.1963-1604270MiAaPQMiAaPQMiAaPQBOOK9910829093403321LC-NMR and other hyphenated NMR techniques3929047UNINA05778nam 22008533u 450 991082160460332120240401191618.03-527-64411-3(CKB)3190000000022655(EBL)1422497(SSID)ssj0000667290(PQKBManifestationID)11378771(PQKBTitleCode)TC0000667290(PQKBWorkID)10674742(PQKB)10746160(MiAaPQ)EBC1422497(OCoLC)779616372(EXLCZ)99319000000002265520131104d2013|||| u|| |engur|n|---|||||txtccrBioelectrochemistry Fundamentals, Applications and Recent Developments1st ed.Hoboken Wiley20131 online resource (413 p.)Advances in Electrochemical Science and Engineering ;v. 13Description based upon print version of record.3-527-32885-8 Advances in Electrochemical Science and Engineering, Volume 13; Contents; Preface; List of Contributors; 1: Amperometric Biosensors; 1.1 Introduction; 1.1.1 Definition of the Term "Biosensor"; 1.1.2 Milestones and Achievements Relevant to Biosensor Research and Development; 1.1.3 "First-Generation" Biosensors; 1.1.4 "Second-Generation" Biosensors; 1.1.5 "Third-Generation" Biosensors; 1.1.6 Reagentless Biosensor Architectures; 1.1.7 Parameters with a Major Impact on Overall Biosensor Response; 1.1.8 Application Areas of Biosensors; 1.2 Criteria for "Good" Biosensor Research1.3 Defining a Standard for Characterizing Biosensor Performances1.4 Success Stories in Biosensor Research; 1.4.1 Direct ET Employed for Biosensors and Biofuel Cells; 1.4.2 Direct ET with Glucose Oxidase; 1.4.3 Mediated ET Employed for Biosensors and Biofuel Cells; 1.4.4 Nanomaterials and Biosensors; 1.4.4.1 Modification of Macroscopic Transducers with Nanomaterials; 1.4.4.2 Nanometric Transducers; 1.4.4.3 Modification of Biomolecules with Nanomaterials; 1.4.5 Implanted Biosensors for Medical Research and Health Check Applications1.4.6 Nucleic Acid-Based Biosensors: Nucleic Acid Chips, Arrays, and Microarrays1.4.7 Immunosensors; 1.4.7.1 Labeled Approaches; 1.4.7.2 Nonlabeled Approaches; 1.5 Conclusion; Acknowledgments; Abbreviations; Glossary; References; 2: Imaging of Single Biomolecules by Scanning Tunneling Microscopy; 2.1 Introduction; 2.2 Interfacial Electron Transfer in Molecular and Protein Film Voltammetry; 2.2.1Theoretical Notions of Interfacial Chemical and Bioelectrochemical Electron Transfer; 2.2.2 Nuclear Reorganization Free Energy2.2.3 Electronic Tunneling Factor in Long-Range Interfacial (Bio)electrochemical Electron Transfer2.3 Theoretical Notions in Bioelectrochemistry towards the Single-Molecule Level; 2.3.1 Biomolecules in Nanoscale Electrochemical Environment; 2.3.2 Theoretical Frameworks and Interfacial Electron Transfer Phenomena; 2.3.2.1 Redox (Bio)molecules in Electrochemical STM and Other Nanogap Configurations; 2.3.2.2 New Interfacial (Bio)electrochemical Electron Transfer Phenomena2.4 In Situ Imaging of Bio-related Molecules and Linker Molecules for Protein Voltammetry with Single-Molecule and Sub-molecular Resolution2.4.1 Imaging of Nucleobases and Electronic Conductivity of Short Oligonucleotides; 2.4.2 Functionalized Alkanethiols and the Amino Acids Cysteine and Homocysteine; 2.4.2.1 Functionalized Alkanethiols as Linkers in Metalloprotein Film Voltammetry; 2.4.2.2 In Situ STM of Cysteine and Homocysteine; 2.4.2.3 Theoretical Computations and STM Image Simulations; 2.4.3 Single-Molecule Imaging of Bio-related Small Redox Molecules2.5 Imaging of Intermediate-Size Biological Structures: Lipid Membranes and InsulinBioelectrochemistry is a fast growing field at the interface between electrochemistry and other sciences such as biochemistry, analytical chemistry and medicinal chemistry. In the recent years, the methods and the understanding of the fundamentals have seen significant progress, which has led to rapid development in the field.Here, the expert editors have carefully selected contributions to best reflect the latest developments in this hot and rapidly growing interdisciplinary topic. The resulting excellent and timely overview of this multifaceted field covers recent methodological advaAdvances in Electrochemical Science and Engineering ;v. 13Bioelectric energy sourcesBioelectrochemistryEnergy metabolism -- PhysiologyHuman Anatomy & PhysiologyHILCCHealth & Biological SciencesHILCCAnimal BiochemistryHILCCBioelectric energy sources.Bioelectrochemistry.Energy metabolism -- Physiology.Human Anatomy & PhysiologyHealth & Biological SciencesAnimal Biochemistry541.3541.37CHE 140fstubCHE 802fstubVE 6300.3rvkVE 6350rvkVN 6050rvkWD 2600rvkCHE802fstubVE6300.3rvkVE6350rvkVN6050rvkAlkire Richard C969181Kolb Dieter M884241Lipkowski Jacek1613178Ross Phil N1613179AU-PeELAU-PeELAU-PeELBOOK9910821604603321Bioelectrochemistry3942340UNINA