05468nam 2200673 450 991082612150332120200520144314.03-527-67341-53-527-67342-33-527-67339-3(CKB)3710000000355746(EBL)1953329(OCoLC)903357111(SSID)ssj0001467518(PQKBManifestationID)11872175(PQKBTitleCode)TC0001467518(PQKBWorkID)11518381(PQKB)10430308(MiAaPQ)EBC1953329(Au-PeEL)EBL1953329(CaPaEBR)ebr11019995(CaONFJC)MIL729454(PPN)19558290X(EXLCZ)99371000000035574620150226h20152015 uy 0engur|n|---|||||txtccrAnalyzing biomolecular interactions by mass spectrometry /edited by Jeroen Kool and Wilfried M. A. NiessenWeinheim, Germany :Wiley-VCH Verlag GmbH & Co.,2015.©20151 online resource (403 p.)Description based upon print version of record.1-322-98172-8 3-527-33464-5 Includes bibliographical references and index.""Analyzing Biomolecular Interactions by Mass Spectrometry""; ""Contents ""; ""List of Contributors ""; ""Preface ""; ""Abbreviations ""; ""Chapter 1 Introduction to Mass Spectrometry, a Tutorial ""; ""1.1 Introduction ""; ""1.2 Figures of Merit ""; ""1.2.1 Introduction ""; ""1.2.2 Resolution ""; ""1.2.3 Mass Accuracy """"1.2.4 General Data Acquisition in MS """"1.3 Analyte Ionization ""; ""1.3.1 Introduction ""; ""1.3.2 Electrospray Ionization ""; ""1.3.3 Matrix-Assisted Laser Desorption Ionization ""; ""1.3.4 Other Ionization Methods ""; ""1.3.5 Solvent and Sample Compatibility Issues """"1.4 Mass Analyzer Building Blocks """"1.4.1 Introduction ""; ""1.4.2 Quadrupole Mass Analyzer ""; ""1.4.3 Ion-Trap Mass Analyzer ""; ""1.4.4 Time-of-Flight Mass Analyzer ""; ""1.4.5 Fourier Transform Ion Cyclotron Resonance Mass Spectrometer ""; ""1.4.6 Orbitrap Mass Analyzer """"1.4.7 Ion Detection """"1.5 Tandem Mass Spectrometry ""; ""1.5.1 Introduction: ""Tandem-in-Time"" and ""Tandem-in-Space"" ""; ""1.5.2 Ion Dissociation Techniques ""; ""1.5.3 Tandem Quadrupole MS-MS Instruments ""; ""1.5.4 Ion-Trap MS n Instruments ""; ""1.5.5 Tandem TOF (TOF-TOF) Instruments """"1.5.6 Hybrid Instruments (Q-TOF, Q-LIT, IT-TOF) """"1.5.7 MS-MS and MSn in FT-ICR-MS ""; ""1.5.8 Orbitrap-Based Hybrid Systems ""; ""1.5.9 Ion-Mobility Spectrometry-Mass Spectrometry ""; ""1.6 Data Interpretation and Analytical Strategies ""; ""1.6.1 Data Acquisition in MS Revisited """"1.6.2 Quantitative Bioanalysis and Residue Analysis ""This monograph reviews all relevant technologies based on mass spectrometry that are used to study or screen biological interactions in general. Arranged in three parts, the text begins by reviewing techniques nowadays almost considered classical, such as affinity chromatography and ultrafiltration, as well as the latest techniques. The second part focusses on all MS-based methods for the study of interactions of proteins with all classes of biomolecules. Besides pull down-based approaches, this section also emphasizes the use of ion mobility MS, capture-compound approaches, chemical proteomiBiomoleculesAnalysisChemistry, AnalyticQuantitativeBiomoleculesAnalysis.Chemistry, AnalyticQuantitative.572.33Kool JeroenNiessen Wilfried M. A.MiAaPQMiAaPQMiAaPQBOOK9910826121503321Analyzing biomolecular interactions by mass spectrometry3960960UNINA