05331nam 2200709 450 991082459330332120220111004710.01-119-01179-51-119-01180-91-119-01402-6(CKB)3710000000366205(EBL)1895913(SSID)ssj0001573276(PQKBManifestationID)16227077(PQKBTitleCode)TC0001573276(PQKBWorkID)14840558(PQKB)11300028(MiAaPQ)EBC1895913(Au-PeEL)EBL1895913(CaPaEBR)ebr11027517(CaONFJC)MIL770120(OCoLC)905919673(PPN)190960620(EXLCZ)99371000000036620520150312h20152015 uy 0engurcnu||||||||txtccrPhotonicsVolume IVBiomedical photonics, spectroscopy, and microscopy. scientific foundations, technology, and applications /edited by David L. Andrews, School of Chemical Sciences University of East Anglia Norwich, UK ; contributors, Thomas Aabo [and thirty three others]Hoboken, New Jersey :Wiley,2015.©20151 online resource (602 p.)A Wiley-Science Wise Co-Publication"A Wiley-Science Wise Co-Publication"--Cover.1-118-22555-4 Includes bibliographical references at the end of each chapters and index.Photonics; Contents; List of Contributors; Preface; 1 Fluorescence; 1.1 Introduction; 1.2 Spectra; 1.2.1 Background and Theory; 1.2.2 Experimental; 1.2.3 Application Example-Melanin Spectra; 1.3 Quantum Yield; 1.3.1 Theory; 1.3.2 Experimental; 1.3.3 Application Example-ThT Detection of Sheet Structure; 1.4 Lifetime; 1.4.1 Theory; 1.4.2 Experimental; 1.4.3 Application Example-In Vivo Glucose Sensing; 1.5 Quenching; 1.5.1 Theory; 1.5.2 Application-Metal Ion Quenching; 1.6 Anisotropy; 1.6.1 Theory; 1.6.2 Experimental; 1.6.3 Application Example-Nanoparticle Metrology; 1.7 Microscopy1.7.1 Systems and Techniques 1.7.2 Application Example-Gold Nanorods in Cells; 1.8 Conclusions; Acknowledgments; 2 Single-Molecule Detection and Spectroscopy; 2.1 Introduction; 2.2 Experimental Setups; 2.2.1 Principles; 2.2.2 Correction of Aberrations; 2.2.3 Polarization Structure at the Focus; 2.2.4 Various Microscopy Methods; 2.3 Fluorescence Spectroscopy; 2.3.1 Introduction, Signal-to-Noise Ratio; 2.3.2 Sample Preparation; 2.3.3 Orientation; 2.3.4 Blinking; 2.3.5 Bleaching; 2.3.6 Superresolution; 2.4 Fluorescence Correlation Spectroscopy; 2.4.1 Photon Counting Histograms, Burst Analysis2.4.2 Fluorescence Correlation Spectroscopy 2.4.3 Multiparameter Analysis; 2.5 Fluorescence Excitation Spectroscopy; 2.5.1 Zero-Phonon Line and Phonon Wing; 2.5.2 Inhomogeneous Broadening; 2.5.3 Hole-Burning [30]; 2.5.4 Single-Molecule Spectroscopy; 2.5.5 Scope of Low-Temperature Single-Molecule Spectroscopy; 2.6 Other Detection Methods; 2.6.1 General Considerations on Signal, Background, and Noise; 2.6.2 Dark-Field Scattering, Total Internal Reflection; 2.6.3 Absorption, Extinction, Interference-Based Methods; 2.6.4 Pump-Probe and Photothermal Detections; 2.7 Conclusion; AcknowledgmentsReferences 3 Resonance Energy Transfer; 3.1 Introduction; 3.2 History of RET; 3.2.1 The First Experiments; 3.2.2 Early Developments of Theory; 3.2.3 Förster Theory; 3.3 The Photophysics of RET; 3.3.1 Primary Excitation Processes; 3.3.2 Coupling of Electronic Transitions; 3.3.3 Dissipation and Line Broadening; 3.3.4 Förster Equation; 3.3.5 Orientation Dependence; 3.3.6 Polarization Features; 3.3.7 Diffusion Effects; 3.3.8 Long-Range Transfer; 3.3.9 Dexter Transfer; 3.4 Investigative Applications of RET in Molecular Biology; 3.4.1 Spectroscopic Ruler; 3.4.2 Conformational Change3.4.3 Intensity-Based Imaging 3.4.4 Lifetime-Based Imaging; 3.4.5 Other Applications; 3.5 The Role of RET in Light-Harvesting Complexes; 3.5.1 Introduction; 3.5.2 Photosynthetic Excitons; Acknowledgments; References; 4 Biophotonics of Photosynthesis; 4.1 Introduction; 4.2 Structure of Pigment-Protein Complexes and Structure-Function Relationships; 4.2.1 Photosystem I (PS I) and Photosystem II (PS II); 4.2.2 PCs of Purple Bacteria; 4.3 Key Concepts in Physics of Pigment-Protein Complexes; 4.3.1 Excitons; 4.3.2 Excitation Energy Transfer4.3.3 Homogeneous and In homogeneous Broadening, Zero-Phonon Lines (ZPLs), and Phonon Sidebands (PSBs)Comprehensive and accessible coverage of the whole of modern photonics Emphasizes processes and applications that specifically exploit photon attributes of light Deals with the rapidly advancing area of modern opticsA Wiley-Science Wise Co-PublicationPhotonicsSpectrum analysisMicroscopyPhotonics.Spectrum analysis.Microscopy.610.28Andrews David L.Aabo ThomasMiAaPQMiAaPQMiAaPQBOOK9910824593303321Photonics2026196UNINA