03886nam 2200553 a 450 991081629190332120240410170151.01-61324-220-4(CKB)2670000000094932(EBL)3019250(SSID)ssj0000521468(PQKBManifestationID)12175889(PQKBTitleCode)TC0000521468(PQKBWorkID)10523043(PQKB)11436599(MiAaPQ)EBC3019250(Au-PeEL)EBL3019250(CaPaEBR)ebr10670815(OCoLC)923660450(EXLCZ)99267000000009493220091222d2010 uy 0engur|n|---|||||txtccrChitosan hydrolysis by non-specific enzymes[electronic resource] /Wenshui Xia and Ping Liu1st ed.New York Nova Science Publishersc20101 online resource (78 p.)Environmental Science, Engineering and TechnologyDescription based upon print version of record.1-61668-152-7 Includes bibliographical references and index.Intro -- CHITOSAN HYDROLYSIS BY NON-SPECIFIC ENZYMES -- CHITOSAN HYDROLYSIS BY NON-SPECIFIC ENZYMES -- CONTENTS -- ABSTRACT -- Chapter 1 INTRODUCTION -- Chapter 2 CHARACTERIZATION OF CHITOSAN HYDROLYSIS BY NONSPECIFIC ENZYMES -- 2.1. CHARACTERIZATION OFCHITOSAN HYDROLYSIS BY NON-SPECIFIC CELLULASES -- (1) Temperature and pH -- (2) Molecular Weight -- (3) Reduction of the Viscosity -- (4) Kinetic Parameters -- (5) Metal Ions -- 2.2. CHARACTERIZATION OF CHITOSAN HYDROLYSIS BY NON-SPECIFIC LIPASE -- 2.3. Characterization of Chitosan Hydrolysis by Non-Specific Papain -- Chapter 3 MECHANISM OF NON-SPECIFIC ENZYMES TOWARD CHITOSAN -- 3.1. PURIFICATION AND CHARACTERIZATION OF CHITOSANOLYTIC COMPONENTS FROM NON -SPECIFIC ENZYMES -- 3.1.1. Purification and Characterization of a Bifunctional Enzyme with Chitosanolytic and Cellulolytic Activity from Commercial Cellulase -- 3.1.2. Purification and Characterization of a Bifunctional Chitosanase with Chitinase Activity from Commercial Lipase -- 3.2. ACTION MODE ANALYSIS OF BIFUNCTIONAL ENZYMES ON CHITOSAN -- 3.2.1. Substrate Specificity -- 3.2.2. Chitosan and Chitooligosaccharide Hydrolysis Analysis by CCBE -- 3.2.3. TLC Analysis of COS Hydrolysis Products by the Purified CNBE -- Chapter 4 STRUCTURE AND FUNCTION ANALYSIS OF BIFUNCTIONAL ENZYMES WITH CHITOSANOLYTIC ACTIVITY -- 4.1. AMINO ACID COMPOSITION OF PURIFIED CCBE AND CNBE -- 4.2. STRUCTURE ANALYSIS OF PURIFIED CCBE AND CNBE -- 4.2.1. N-Terminal Sequencing Analysis of CNBE -- 4.2.2. Sequencing Analysis of the Purified CCBE by MALDI-TOF Mass -- 4.3. DETERMINATION OF THE ACTIVE SITES OF CCBE AND CNBE BY CHEMICAL MODIFICATION -- 4.3.1. The Essential Carboxyl Group Modification using EDC -- 4.3.2. Modification of Tryptophan Residues using NBS -- 4.3.3. Modification of Histidine Residue using DEPC.4.3.4. Modification of Tyrosine, Threonine/Serine, Arginine, Methionine Residues Etc -- Chapter 5 IDENTIFICATION OF CCBE FROM T.VIRIDE BY MOLECULAR CLONING -- 5.1. DETERMINATION OF FERMENTATION CONDITION OF T.VIRIDE -- 5.2. CLONING AND SEQUENCE ANALYSIS OF CCBE GENE -- 5.3. EXPRESSION ANALYSIS OF CCBE SPLICING GENES IN PICHIA PATORIS -- ACKNOWLEDGMENTS -- REFERENCE -- INDEX -- Blank Page.Environmental Science, Engineering and TechnologyChitosanMetabolismChitosanMetabolism.612.3/9Xia Wenshui1687143Liu Ping1687144MiAaPQMiAaPQMiAaPQBOOK9910816291903321Chitosan hydrolysis by non-specific enzymes4060386UNINA