04231nam 2200709Ia 450 991081100770332120241213142137.097866133109729781283310970128331097X97801238554590123855454(CKB)2550000000063471(EBL)802466(OCoLC)769342081(SSID)ssj0000591978(PQKBManifestationID)11352048(PQKBTitleCode)TC0000591978(PQKBWorkID)10728666(PQKB)11568203(Au-PeEL)EBL802466(CaPaEBR)ebr10509200(CaONFJC)MIL331097(PPN)182565076(MiAaPQ)EBC802466(PPN)176592040(EXLCZ)99255000000006347120111109d2012 uy 0engurcn|||||||||txtrdacontentstirdacontentcrdamediacrrdacarrierMolecular biology techniques a classroom laboratory manual /Susan Carson, Heather Miller, D. Scott Witherow3rd ed.Amsterdam ;Boston Elsevier /Academic Pressc20121 online resource (xxvi, 200 pages) color illustrationsIncludes index.Previous edition: 2006.9780123855442 0123855446 Includes bibliographical references and index.Lab Session 1 Getting Oriented: Practicing with Micropipettes -- Lab Session 2 Purification and Digestion of Plasmid (Vector) DNA -- Lab Session 3 PCR Amplification of egfp and Completion of Vector Preparation -- Lab Session 4 Preparation of Insert DNA (egfp) PCR Product -- Lab Session 5 DNA Ligation and Transformation of Escherichia coli -- Lab Session 6 Colony Hybridization -- Lab Session 7 Characterization of Recombinant Clones: Part 1 -- Lab Session 8 Characterization of Recombinant Clones: Part 2 -- Lab Session 9 Characterization of Recombinant Clones: Part 3 -- Lab Session 10 Computational Analysis of DNA Sequence from a Positive Clone: Part 2 -- Lab Session 11 Expression of Fusion Protein from Positive Clones, SDS-PAGE and Western Blot: Part 1 -- Lab Session 12 Expression of Fusion Protein from Positive Clones, SDS-PAGE and Western Blot: Part 2 -- Lab Session 13 Extraction of Recombinant Protein from Escherichia coli Using a Glutathione Affinity Column -- Lab Session 14 Analysis of Purification Fractions -- Lab Session 15 Total RNA Purification -- Lab Session 16 Analysis of gst::egfp mRNA Levels by RT-qPCR: Part 1 -- Lab Session 17 Analysis of gst::egfp mRNA Levels by RT-qPCR: Part 2 -- Lab Session 18 Analysis of gst::egfp mRNA Levels by Semi-Quantitative RT-PCR: Part 1 -- Lab Session 19 Analysis of gst::egfp mRNA Levels by Semi-Quantitative RT-PCR: Part 2 -- Appendix 1 Equipment -- Appendix 2 Prep List -- Appendix 3 Preparation of Competent E. coli Cells -- Appendix 4 Pre-Lab Questions.This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein.The second edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester,Molecular biologyLaboratory manualsMolecular biologyTechniqueHandmooks, manuals, etcMolecular biologyMolecular biologyTechnique572.8/078572.8078572.8078Carson SueDr.1632298Miller Heather B1779216Witherow D. Scott1779215MiAaPQMiAaPQMiAaPQBOOK9910811007703321Molecular biology techniques4302466UNINA