04421nam 2200565 450 991080730640332120230808194351.01-119-33016-51-119-33017-31-119-33014-9(CKB)3710000000765789(EBL)4692004(MiAaPQ)EBC4692004(Au-PeEL)EBL4692004(CaPaEBR)ebr11268666(CaONFJC)MIL957429(OCoLC)956672082(EXLCZ)99371000000076578920160513d2016 uy| 0engur|n|---|||||rdacontentrdamediardacarrierLive cell assays from research to health and regulatory applications /Christophe FurgerHoboken, New Jersey :ISTE Ltd/John Wiley and Sons Inc,2016.1 online resource (285 p.)Biomedical engineering seriesDescription based upon print version of record.1-84821-858-3 Cover; Title Page; Copyright; Contents; Foreword; A researcher's view; A consultant's view; The author; Acknowledgments; Abbreviations; List of Cell Assays; Introduction; 1: Principles and Position; 1.1. Live cell assay principles; 1.2. Application areas; 1.3. Positioning; 1.3.1. Definition and typology of cell tests; 1.3.2. The regulatory and industrial dimension; 1.4. Market; 1.5. Competitive advantages; 1.5.1. Cells are live information models; 1.5.2. Development: high throughput; 1.5.3. Development: multiplex analysis; 1.5.4. Development: miniaturization1.5.5. Development: molecular engineering1.5.6. Development: standardization; 1.6. Can measurements of cells in culture be extrapolated to effects in the organism?; 1.6.1. Toxicokinetics; 1.6.2. Components of the immune system; 1.6.3. Biotransformation; 1.6.4. The macrocellular environment; 1.7. Limits; 1.7.1. Importance of cellular microenvironment; 1.7.2. Other limits; 2: History and State of the Art; 2.1. Origins of cell culture; 2.1.1. Pioneering studies; 2.1.2. Alexis Carrel; 2.1.3. Were Dr Carrel's cells immortal?; 2.2. The HeLa line and the first applications of cell culture2.2.1. A vaccine against poliomyelitis2.2.2. Cells in space; 2.2.3. Cell cloning; 2.3. New cell lines; 2.3.1. The CHO line; 2.3.2. An increasing number of cell lines; 2.4. Cross-contamination; 2.5. Cell lines, an ethical issue; 2.6. The first generation of cell assays (1969-1983); 2.6.1. The karyotype test; 2.6.2. The MTT assay; 2.6.3. The NRU test; 2.7. The first target of regulatory assays: genotoxicity (1983-1986); 2.7.1. Ames test (OECD guideline 471); 2.7.2. In vitro mammalian chromosome aberration test (OECD guideline 473)2.7.3. In vitro mammalian cell gene mutation test (OECD guideline 476)2.7.4. In vitro sister chromatid exchange assay in mammalian cells (OECD guideline no. 479); 2.7.5. DNA damage and repair, unscheduled DNA synthesis in mammalian cells (OECD guideline 482); 3: Cell Models and Technologies; 3.1. Fluorescence and bioluminescence; 3.1.1. Green fluorescent protein; 3.1.2. BRET; 3.1.3. FRET; 3.1.4. Other applications of GFP; 3.1.5. The reporter gene approach; 3.2. Impedance variation in cell population; 3.3. Optical signals modified by state of cells; 3.4. Cellular autofluorescence3.4.1. The case of chlorophyll3.5. The different cell models and culture modes available; 3.5.1. Immortalized lines; 3.5.2. Primary cells; 3.5.3. Three-dimensional cell culture; 4: Loss of Cell Homeostasis: Applications in Toxicity Measurement; 4.1. What relevant information to use in the living cell?; 4.2. Lysosomal activity; 4.3. Redox balance and oxidative stress; 4.4. Integrity of the plasma membrane; 4.5. Cellular efflux; 4.6. Homeostasis of ion exchanges; 4.6.1. The calcium ion; 4.6.2. Maintenance of membrane potential; 4.7. Metabolism and cell respiratory activity; 4.8. Genotoxicity4.9. ApoptosisMcGraw-Hill's biomedical engineering series.CellsBiological assayCells.Biological assay.571.6Furger Christophe960179MiAaPQMiAaPQMiAaPQBOOK9910807306403321Live cell assays4045072UNINA