02605oam 2200601I 450 991080020000332120230803030144.00-429-11045-61-4398-4813-010.1201/b14930 (CKB)2670000000359900(EBL)1190579(SSID)ssj0000876789(PQKBManifestationID)11467572(PQKBTitleCode)TC0000876789(PQKBWorkID)10904439(PQKB)10259615(MiAaPQ)EBC1190579(Au-PeEL)EBL1190579(CaPaEBR)ebr10706067(CaONFJC)MIL502331(OCoLC)845257664(OCoLC)855711925(EXLCZ)99267000000035990020180331d2013 uy 0engur|n|---|||||txtccrPCR technology current innovations /edited by Tania Nolan, Stephen A. Bustin3rd ed.Boca Raton :Taylor & Francis,2013.1 online resource (457 p.)Description based upon print version of record.1-4398-4805-X Includes bibliographical references.pt. 1. Template preparation -- pt. 2. Reaction components -- pt. 3. Instruments -- pt. 4. Design, optimization, QC, and standardization -- pt. 5. Data analysis -- pt. 6. Applications.A technique used to amplify the number of copies of a specific region of DNA, the polymerase chain reaction (PCR) is at the forefront of the dramatic development of biochemistry. This text provides the tools for developing innovative approaches to using this leading technology. It includes theoretical considerations, discussions, and a selection of state-of-the-art techniques for mutation studies, clinical diagnosis, and the detection of food-borne pathogens. This edition also discusses the preparation of PCR experiments, includes examples of analytical PCR divided into qualitative and quantitative applications, and explores preparative methods that address DNA generation for further analysis and in vitro evolution--Provided by publisher.DNA polymerasesTransferasesDNA polymerases.Transferases.572.8/636Nolan Tania1588503Bustin Stephen A.1954-1588504MiAaPQMiAaPQMiAaPQBOOK9910800200003321PCR technology3878376UNINA