03762oam 2200649I 450 991079996830332120230725024607.01-4398-5905-10-429-14677-91-4200-7350-810.1201/EBK1420073492 (CKB)2670000000035693(EBL)565953(OCoLC)660536815(SSID)ssj0000410739(PQKBManifestationID)11309157(PQKBTitleCode)TC0000410739(PQKBWorkID)10351518(PQKB)11679618(MiAaPQ)EBC565953(Au-PeEL)EBL565953(CaPaEBR)ebr10408382(CaONFJC)MIL693918(OCoLC)680620466(EXLCZ)99267000000003569320180331d2010 uy 0engur|n|---|||||txtccr3D cell-based biosensors in drug discovery programs microtissue engineering for high throughput screening /William S. KisaalitaBoca Raton [Fla.] :CRC Press,2010.1 online resource (392 p.)Description based upon print version of record.1-4398-5904-3 1-322-62636-7 1-4200-7349-4 Includes bibliographical references and index.Front Cover; Dedication; Contents; Preface; Author; Part I. Introduction; Chapter 1. Biosensors and Bioassays; Chapter 2. Target-Driven Drug Discovery; Part II. 3D versus 2D Cultures; Chapter 3. Comparative Transcriptional Profiling and Proteomics; Chapter 4. Comparative Structure and Function; Part III. Emerging Design Principles; Chapter 5. Chemical Microenvironmental Factors; Chapter 6. Spatial and Temporal Microenvironmental Factors; Chapter 7. Material Physical Property and Force Microenvironmental Factors; Chapter 8. Proteomics as a Promising Tool in the Search for 3D BiomarkersChapter 9. Readout Present and Near FutureChapter 10. Ready-to-Use Commercial 3D Plates; Part IV. Technology Deployment Challenges and Opportunities; Chapter 11. Challenges to Adopting 3D Cultures in HTS Programs; Chapter 12. Cases for 3D Cultures in Drug Discovery; Chapter 13. Ideal Case Study Design; Appendix A: Patents for 3D Scaffolds; Appendix B: Current Drug Targets; Appendix C: Popular Cell Lines in Drug Discovery; Appendix D: Stem Cells in Drug Discovery; Back CoverThis book is based upon cutting-edge research conducted in the authors lab (Cellular Bioengineering), which over the past decade has developed a number of sophisticated techniques to facilitate use of 3D cell based assays or biosensors. This book uses data from peer-reviewed publications to conclusively justify use of 3D cell cultures in cell-based biosensors (assays) for (HTS). The majority of assays performed in accelerated drug discovery processes are biochemical in nature, but there is a growing demand for live cell-based assays. Unlike biochemical ones, cellular assays are functional approximations of in vivo biological conditions and can provide more biologically relevant information--Provided by publisher.Pharmaceutical biotechnologyBiosensorsHigh throughput screening (Drug development)Pharmaceutical biotechnology.Biosensors.High throughput screening (Drug development)615/.19Kisaalita William Ssempa1953,1587366MiAaPQMiAaPQMiAaPQBOOK99107999683033213D cell-based biosensors in drug discovery programs3875086UNINA