03276oam 2200553I 450 991079726770332120230807215624.00-429-16192-11-4822-3557-910.1201/b18477 (CKB)3710000000413966(EBL)1839844(SSID)ssj0001542352(PQKBManifestationID)16128996(PQKBTitleCode)TC0001542352(PQKBWorkID)14791684(PQKB)10574923(MiAaPQ)EBC1839844(OCoLC)908931921(EXLCZ)99371000000041396620180331h20152015 uy 0engur|n|---|||||txtccrCyclic nucleotide signaling /edited by Xiaodong ChengBoca Raton, Florida :CRC Press,[2015]©20151 online resource (282 p.)Methods in Signal Transduction SeriesDescription based upon print version of record.1-4822-3556-0 Includes bibliographical references at the end of each chapters.Front Cover; Contents; Series Preface; Preface; Editor; Contributors; Chapter 1: Discovery of Small Molecule EPAC Specific Modulators by High-Throughput Screening; Chapter 2: Cyclic Nucleotide Analogs as Pharmacological Tools for Studying Signaling Pathways; Chapter 3: High-Throughput FRET Assays for Fast Time-Dependent Detection of Cyclic AMP in Pancreatic β Cells; Chapter 4: Assessing Cyclic Nucleotide Recognition in Cells : Opportunities and Pitfalls for Selective Receptor Activation; Chapter 5: Monitoring Cyclic Nucleotides Using Genetically Encoded Fluorescent ReportersChapter 6: Structural Characterization of Epac by X-Ray CrystallographyChapter 7: Sensory Neuron cAMP Signaling in Chronic Pain; Chapter 8: Monitoring Real-Time Cyclic Nucleotide Dynamics in Subcellular Microdomains; Chapter 9: Identifying Complexes of Adenylyl Cyclase with A-Kinase Anchoring Proteins; Chapter 10: Assessing Cyclic Nucleotide Binding Domain Allostery and Dynamics by NMR Spectroscopy; Chapter 11: A Protocol for Expression and Purification of Cyclic Nucleotide-Free Protein in Escherichia coli; Chapter 12: Cyclic Nucleotide Analogues as Chemical Tools for Interaction AnalysisChapter 13: Dissecting the Physiological Functions of PKA Using Genetically Modified MiceBack Cover<P>Showcasing the recent progresses of the field, <B>Cyclic Nucleotide Signaling<I> </B></I>covers the major tools and methodologies used in various areas of research. The majority of the chapters are protocol oriented, with the goal of providing clear directions for laboratory use. Students and investigators new to the field will find this book particularly informative, as will scientists already actively researching nucleotide signaling.</P>Methods in signal transduction.Cyclic nucleotidesCyclic nucleotides.571.7/4Cheng XiaodongFlBoTFGFlBoTFGBOOK9910797267703321Cyclic nucleotide signaling3783577UNINA