05145nam 2200613 450 991078742950332120230807213602.01-910190-04-7(CKB)3710000000355112(EBL)1977391(SSID)ssj0001466834(PQKBManifestationID)11831245(PQKBTitleCode)TC0001466834(PQKBWorkID)11504597(PQKB)10032986(MiAaPQ)EBC1977391(MiAaPQ)EBC5897829(Au-PeEL)EBL5897829(OCoLC)904518280(EXLCZ)99371000000035511220191011d2015 uy 0engur|n|---|||||txtccrAdvanced vaccine research methods for the decade of vaccines /edited by Fabio Bagnoli and Rino RappuoliNorfolk, England :Caister Academic Press,[2015]©20151 online resource (474 p.)Description based upon print version of record.1-910190-03-9 Includes bibliographical references and index.Contents; Contributors; Current Books of Interest; Preface; Part I: Innovative Technologies and Approaches in Vaccine Research; 1: Deep Sequencing in Vaccine Research, Development and Surveillance ; Impact of DNA sequencing on vaccines research and development; Deep sequencing potential to transform vaccinology; 2: New Bioinformatics Algorithms Applied to Deep Sequencing Projects ; Introduction; Better understating of host and pathogen genomes; Computational approaches to study adaptive immune responses; Better understanding of transcriptomeEpigenomics: understanding of inheritable chemical changesMetagenomics: finding new pathogens and strains; Bioinformatics challenges; 3: Comparative Genomics Approaches for Tracking the Emergence and Spread of Disease-associated Bacteria; Background; Comparative genomics of disease-associated bacteria; Assays for identification of disease-associated bacteria based on insights from comparative genomics; Conclusions; 4: Quantitative Proteomics in Vaccine Research; Introduction; Quantitative proteomics based on gel electrophoresis; Quantitative proteomics based on differential mass tagLabel-free technologies and targeted proteomicsMass spectrometry for viral vaccinology; Proteomic technology for vaccine development ; Combining proteomics and system biology to improve vaccines; Concluding remarks; 5: Structural Biology in Vaccine Research; Introduction; Experimental mapping and computational prediction of epitopes; Examples of structural vaccinology for bacterial and viral pathogens; Concluding remarks and future directions; 6: Cellular Screens to Interrogate the Human T- and B-cell Repertoires and Design Better Vaccines ; IntroductionGeneration of naive B- and T-cell repertoires7: Novel Strategies of Vaccine Administration: The Science Behind Epidermal and Dermal Immunization; Introduction; The skin layers; Skin antigen-presenting cells and other players in vaccination; Principles and methods in skin immunization; Vaccine formulation and immunity; Immunological advantage of epidermal and dermal immunization; 8: Toll-like Receptors as Targets to Develop Novel Adjuvants; Introduction; Innate immunity; TLR-based adjuvants in high-priority vaccines; Lessons learned from studies of TLR agonists as adjuvantsSafety considerations of TLR agonistsChallenges for the next decade; 9: The Importance of Cell-mediated Immunity for Bacterial Vaccines ; Introduction; T-cell responses to bacterial infection; Targeting cellular immunity for novel anti-bacterial vaccine design; 10: T-cell-inducing Vaccines; Introduction; How can we measure T-cell responses?; Technologies for inducing T-cell responses by vaccination; Heterologous prime-boost immunization; Successful clinical development of T-cell vaccines; 11: Exploiting the Mutanome for Personalized Cancer ImmunotherapyCancer mutations as therapeutic targetsSince the publication of our first book book ""Vaccine Design: Innovative Approaches and Novel Strategies"" in 2011, the field of vaccinology has advanced significantly. The application of new sophisticated 'omics' technologies and the use of pioneering approaches have yielded a wealth of new data. This new book aims to distill the most important new findings to provide a timely overview of the field. Written by leading experts in the field, each chapter affords a critical insight to a particular topic, reviews current research, discusses future direction and aims to stimulate discussion. The VaccinesResearchDrug developmentVaccinesResearch.Drug development.615.372Bagnoli FabioRappuoli RinoMiAaPQMiAaPQMiAaPQBOOK9910787429503321Advanced vaccine research3743866UNINA