04501nam 2200577 a 450 991078646480332120230120012138.00-323-15913-31-299-19350-1(CKB)2670000000284022(EBL)1129861(OCoLC)829460454(SSID)ssj0000785562(PQKBManifestationID)12281984(PQKBTitleCode)TC0000785562(PQKBWorkID)10795385(PQKB)10300824(MiAaPQ)EBC1129861(EXLCZ)99267000000028402219890825d1990 uy 0engur|n|---|||||txtccrBiotechnology[electronic resource] a laboratory course /Jeffrey M. Becker, Guy A. Caldwell, Eve Ann ZachgoSan Diego Academic Pressc19901 online resource (251 p.)Description based upon print version of record.0-12-084560-1 Includes bibliographical references and index.Front Cover; Biotechnology: A Laboratory Course; Copyright Page; Dedication; Table of Contents; Preface; Acknowledgments; Suggested Schedule for Exercises; Record Keeping and Safety Rules; Format of Student Laboratory Records; Ten Dos and Don'ts of Record Keeping; Criteria for Grading the Laboratory Notebook; Safety Rules in the Laboratory; Chapter 1. Measurement of pH; Introduction; Reagents/Supplies; Equipment; Procedure; References; Questions; Chapter 2. Use of Micropipettors and Spectrophotometers; Introduction; Reagents/Supplies; Equipment; Procedure; References; QuestionsChapter 3. Aseptic Technique: Transferring a CultureIntroduction; Reagents/Supplies; Equipment; Procedure; Questions; Chapter 4. Establishing a Pure Culture: The Streak Plate; Introduction; Reagents/Supplies; Equipment; Procedure; Questions; Chapter 5. Preparation of Culture Media; Introduction; Reagents/Supplies; Equipment; Procedure; References; Questions; Chapter 6. The Growth Curve; Introduction; Reagents/Supplies; Equipment; Procedure; Data Collection and Results; References; Questions; Chapter 7. Isolation of Plasmid DNA from Escherichia coli: The Mini-Prep; IntroductionReagents/SuppliesEquipment; Procedure; References; Questions; Chapter 8. Purification, Concentration, and Quantitation of DNA; Introduction; PART A PURIFICATION OF PLASMID DNA BY SOLVENT EXTRACTION; Reagents/Supplies; Equipment; Procedure; PART Β CONCENTRATION OF PLASMID DNA; Reagents/Supplies; Equipment; Procedure; PART C QUANTITATION OF DNA; Reagents/Supplies; Equipment; Procedure; References; Questions; Chapter 9. Isolation of Plasmid DNA: The Maxi-Prep; Introduction; Reagents/Supplies; Equipment; Procedure; References; QuestionsChapter 10. Restriction Digestion and Agarose Gel ElectrophoresisIntroduction; Reagents/Supplies; Equipment; Procedure; References; Questions; Chapter 11. Southern Transfer; Introduction; Reagents/Supplies; Equipment; Procedure; References; Questions; Chapter 12. Preparation, Purification, and Hybridization of Probe; Introduction; Reagents/Supplies; Equipment; Procedure; References; Questions; Chapter 13. Transformation of Saccharomyces cerevisiae; Introduction; Reagents/Supplies; Equipment; Procedure; References; Questions; Chapter 14. Transformation of Escherichia coli by Plasmid DNAIntroductionReagents/Supplies; Equipment; Procedure; References; Questions; Chapter 15. Protein Assays; Introduction; PART A LOWRY PROTEIN ASSAY; Reagents/Supplies; Equipment; Procedure; PART Β BRADFORD PROTEIN ASSAY; Reagents/Supplies; Equipment; Procedure; References; Question; Chapter 16. β-Galaetosidase Assay; Introduction; Reagents/Supplies; Equipment; Procedure; References; Questions; Chapter 17. Determination of β-Galactosidase in Permeabilized Yeast Cells; Introduction; Reagents/Supplies; Equipment; Procedure; References; QuestionsChapter 18. Assay of β-Galactosidase in Cell ExtractsBiotechnologyBiotechnologyLaboratory manualsBiotechnology660.6Becker Jeffrey M621784Caldwell Guy A1500534Zachgo Eve Ann1500535MiAaPQMiAaPQMiAaPQBOOK9910786464803321Biotechnology3727277UNINA