03984nam 2200589Ia 450 991078401730332120200520144314.01-280-63005-197866106300590-08-045885-8(CKB)1000000000336820(EBL)269663(OCoLC)162597957(SSID)ssj0000225796(PQKBManifestationID)11187096(PQKBTitleCode)TC0000225796(PQKBWorkID)10230303(PQKB)10095229(Au-PeEL)EBL269663(CaPaEBR)ebr10139548(CaONFJC)MIL63005(MiAaPQ)EBC269663(EXLCZ)99100000000033682020050504d2006 uy 0engur|n|---|||||txtccrA practical handbook of preparative HPLC[electronic resource] /Donald A. WellingsOxford Elsevier20061 online resource (193 p.)Description based upon print version of record.1-85617-466-2 Includes bibliographical references and index.Cover; copyright; Contents; front matter; Preface; Foreword; Abbreviations; body; 1 The history and development of preparative HPLC; 2 Fluid dynamics, mass transport and friction; 3 Modes of chromatographic separation; Particle size; Pore structure, size and surface area; 3.1 Normal phase chromatography; 3.2 Reversed phase chromatography; 3.3 Chiral separations; Chirality; Optical purity; Separation of enantiomers; 3.4 Ion exchange chromatography; 3.5 Exclusion chromatography; 3.6 Affinity chromatography; 4 How to get started; 4.1 Packing a column; Column packing protocol for column type 1Slurry concentrationSlurry and column preparation; Column packing protocol for column type 2; 4.2 What and where!; 4.3 Product recovery; 4.4 Productivity; 5 Process development and optimization; 5.1 Sample self displacement for purification of a peptide; Analytical HPLC; Gradient selection; Loading study; Flow rate optimization; 5.2 Boxcar injections for chiral separations; 6 Documentation and record keeping; 6.1 Equipment qualification; 6.2 Process documentation; back matter; Appendices; PROCESS INSTRUCTIONS; CONTENTS; DEVIATION SHEET; OPERATION 1 PREPARATION SECTION; 1.01 Abbreviations1.02 Target Specifications1.03 Raw Materials; 1.04 Equipment; 1.05 Consumables; 1.06 In-Process Tests; Analytical HPLC Conditions; Analytical HPLC Strength Method; 1.07 Ancillary SOPs; 1.08 Cleaning; 1.09 Personnel and Responsibilities; 1.10 Safety; Safety Precautions; OPERATION 2 PRE-PURIFICATION COLUMN PREPARATION; OPERATION 3 PURIFICATION; PURIFICATION DEVIATION SHEET; EXAMPLE OF FRACTION RECONCILIATION DOCUMENT; OPERATION 1 SUMMARY; References; CHAPTER 1; CHAPTER 2; CHAPTER 3; CHAPTER 4; CHAPTER 5; CHAPTER 6; IndexThis book is a distillation of twenty years of practical experience of the high pressure liquid chromatography (HPLC) process. Deliberately steering clear of complex theoretical aspects, this book concentrates on the everyday problems associated with the technique, making it perfect for frequent use in the laboratory and for those in the pharmaceutical, agrochemical and biotechnology industries for the analysis and purification of drugs, small molecules, proteins and DNA.This book...Provides practical, hands-on advice based on years of experienceWill help ensure optimal design,High performance liquid chromatographyLiquid chromatographyHigh performance liquid chromatography.Liquid chromatography.543.84Wellings Donald A1543587MiAaPQMiAaPQMiAaPQBOOK9910784017303321A practical handbook of preparative HPLC3797140UNINA