05649nam 2200733 a 450 991078072810332120230725041509.01-282-75824-19786612758249981-4277-19-3(CKB)2490000000001593(EBL)1679396(OCoLC)850858647(SSID)ssj0000419589(PQKBManifestationID)12130972(PQKBTitleCode)TC0000419589(PQKBWorkID)10382957(PQKB)10121739(MiAaPQ)EBC1679396(WSP)00000567 (Au-PeEL)EBL1679396(CaPaEBR)ebr10421998(CaONFJC)MIL275824(EXLCZ)99249000000000159320100105d2010 uy 0engur|n|---|||||txtccrHigh-performance liquid chromatography and mass spectrometry of porphyrins, chlorophylls and bilins[electronic resource] /Chang Kee LimRiver Edge, N.J. World Scientific20101 online resource (245 p.)Methods in chromatography ;v. 2Description based upon print version of record.981-02-3068-0 Includes bibliographical references and index.Contents; Preface; Chapter 1. Structure, Distribution, Biosynthesis and Function; 1.1. Introduction; 1.2. Nomenclature; 1.3. Biosynthesis of Porphyrins, Haem and Chlorophyll; 1.4. Biosynthesis of Bilins in Animals and Plants; 1.4.1. Degradation of haem to bile pigments; 1.4.2. Biosynthesis of bilins in plants, algae and cyanobacteria; 1.4.3. Degradation of chlorophyll in senescent higher plants; 1.5. Function of Porphyrins and Other Tetrapyrroles; References; Chapter 2. High-Performance Liquid Chromatography of Porphyrins; 2.1. Introduction; 2.2. HPLC of Porphyrin Methyl Esters2.3. HPLC of Porphyrin Free Acids2.3.1. Stationary phases for reversed-phase HPLC of porphyrins; 2.3.2. Mobile phases for reversed-phase HPLC of porphyrins; 2.3.3. Procedure for optimisation of porphyrin separation by reversed-phase HPLC; 2.3.4. Retention behaviour of porphyrins in reversed-phase HPLC; 2.4 HPLC of Porphyrinogens; References; Chapter 3. Mass Spectrometry of Porphyrins; 3.1. Introduction; 3.2. Fast Atom Bombardment (FAB) Mass Spectrometry of Porphyrins; 3.3. Laser Desorption/Ionisation Time-of-Flight (LDI-TOF) Mass Spectrometry of Porphyrins3.4 Electrospray Ionisation Mass Spectrometry (ESI-MS) and HPLC/ESI-MS of Porphyrins3.4.1. HPLC/ESI-MS of porphyrins; 3.4.2. ESI-MS/MS fragmentation of porphyrins; 3.4.2.1. ESI-MS/MS product ion spectrum and fragmentation pathways of uroporphyrin; 3.4.3. ESI-MS/MS fragmentation of hydroxyuroporphyrins; 3.4.3.1. ESI-MS/MS product ion spectrum and fragmentation pathways of meso-hydroxyuroporphyrin; 3.4.3.2. ESI-MS/MS product ion spectrum of hydroxyacetic acid uroporphyrin; 3.4.3.3. ESI-MS/MS product ion spectrum of α-hydroxypropionic acid uroporphyrin3.4.3.4. ESI-MS/MS product ion spectrum and fragmentation pathways of β-hydroxypropionic acid uroporphyrin3.4.3.5. ESI-MS/MS product ion spectra and fragmentation pathways of cis- and transhydroxyspirolactoneurochlorins; 3.4.3.6. ESI-MS/MS product ion spectra and fragmentation pathways of cis- and transdihydroxyurochlorins; 3.4.3.7. Characteristic ESI-MS/MS product ions of hydroxyuroporphyrins; 3.4.4. ESI-MS/MS product ion spectra of ketoacid uroporphyrins; 3.4.5. ESI-MS/MS of heptacarboxylic porphyrin; 3.4.6. ESI-MS/MS of hydroxyheptacarboxylic porphyrins3.4.7. ESI-MS/MS of ketoacid heptacarboxylic porphyrins and formyl heptacarboxylic porphyrin3.4.8. ESI-MS/MS product ion spectrum and fragmentation pattern of coproporphyrin; 3.4.9. ESI-MS/MS of hydroxymethylcoproporphyrin; 3.4.10. ESI-MS/MS of β-ketopropionic acid coproporphyrin and formylcoproporphyrin; 3.4.11. ESI-MS/MS product ion spectrum and fragmentation pattern of protoporphyrin; 3.4.12. HPLC/ESI-MS and MS/MS of meso-tetraphenylporphyrin derivatives; References; Chapter 4. Porphyrin Profiles in Blood, Urine and Faeces by HPLC and HPLC/ESI-MS; 4.1. Introduction4.2. Sample Collection and Preparation The porphyrins, chlorophylls, bilins and related tetrapyrroles are vital for all living organisms. Natural and synthetic tetrapyrroles are used extensively in foods, cosmetics, biotechnology, pharmaceuticals, diagnostics and medicine. Methods for their separation and characterization therefore, have a very wide area of applications. Yet, there is a dearth of books dedicated to HPLC and HPLC/MS of tetrapyrroles. Lim addresses this problem admirably by providing practical HPLC and HPLC/MS protocols coupled with in-depth chromatographic and mass spectrometric reference data. These are invaluableMethods in chromatography ;v. 2.PorphyrinsChlorophyllTetrapyrrolesHigh performance liquid chromatographyMass spectrometryPorphyrins.Chlorophyll.Tetrapyrroles.High performance liquid chromatography.Mass spectrometry.547/.593Lim Chang Kee1519850MiAaPQMiAaPQMiAaPQBOOK9910780728103321High-performance liquid chromatography and mass spectrometry of porphyrins, chlorophylls and bilins3758158UNINA01581nam0 22003371i 450 UON0021485720231205103349.51720-7029-347-520030730d1976 |0itac50 bafreFR|||| 1||||ˆL'‰échange symbolique et la mortJean BaudrillardParisGallimardc1976347 p.23 cm.001UON000419272001 Bibliotheque des Sciences HumainesMORTEUONC051298FIPsicologia socialeUONC017234FISIMBOLISMOUONC006896FIFRParisUONL002984302Interazione sociale21BaudrillardJeanUONV11908235505GallimardUONV246610650ITSOL20251003RICAUON00214857SIBA - SISTEMA BIBLIOTECARIO DI ATENEOSI Angl Smarrito Smarrito non disponibile SI LO 66814 5 non disponibile SmarritoSIBA - SISTEMA BIBLIOTECARIO DI ATENEOSI Angl VII 0333 SI LO 35828 5 0333 SIBA - SISTEMA BIBLIOTECARIO DI ATENEOSI VII 021 SI LO 29858 7 021 SIBA - SISTEMA BIBLIOTECARIO DI ATENEOSI B F 0078 SI SC 5861 5 0078 Echange symbolique et la mort18081UNIOR