04981nam 2200553 a 450 991078011460332120230421041550.01-283-66474-70-19-159161-00-585-48392-2(CKB)111087026789928(EBL)1043143(OCoLC)815671912(MiAaPQ)EBC1043143(Au-PeEL)EBL1043143(CaPaEBR)ebr10612575(EXLCZ)9911108702678992819970311d1997 uy 0engur|n|---|||||Subcellular fractionation[electronic resource] a practical approach /edited by J.M. Graham and D. RickwoodOxford ;New York IRL Press at Oxford University Pressc19971 online resource (360 p.)The practical approach seriesDescription based upon print version of record.0-19-963494-7 Includes bibliographical references and index.Cover; Contents; List of contributors; Abbreviations; 1. Homogenization of tissues and cells; 1. Introduction; 2. Aims of the homogenization procedure; 3. Influence of sample type; 4. Homogenization media; 5. Methods of homogenization; Type 1 homogenizers; Type 2 homogenizers; 6. Homogenization of tissues and cells; Mammalian liver; Brain; Muscle; Mammalian tissue culture cells; Plant organelles; Yeast; Other fungi and algae; Trypanosomes; Bacteria; References; 2. Isolation of subcellular fractions; 1. Introduction; 2. Composition of a tissue homogenate; 3. Properties of cell organellesFactors affecting organelle density and size4. Centrifugal methods for the separation of organelles; Separation by size; Separation by density; Density perturbation; 5. Non-centrifugal procedures; Immunoisolation; Separation by electrophoresis; 6. Identification of separated material; Marker enzymes; Introduced markers for endocytic and exocytic pathways; Characteristic non-enzymatic proteins; 7. Assessment of the purity of fractions; Purity and purification; Problems from cell heterogeneity within tissues; Problems arising from organelle fragmentationMissorting in the exocytic and endocytic pathways8. Fractionation problems; No separation; Aggregation following resuspension of a fraction; Poor recovery of markers; Damage to cell structures; 9. A systematic approach to cell fractionation; Preliminary studies; Determination of the properties of components of the homogenate; Method development; Simplification of the separation; References; 3. Isolation and characterization of nuclei and nuclear subfractions; 1. Introduction; 2. Methods of preparing purified nuclei; Types of cells and tissue samples; Homogenization mediaHomogenization methodsCentrifugation conditions; Assays of nuclear purity; 3. Methods for purifying metaphase chromosomes; 4. Isolation of nuclear subfractions; Preparation of nucleoli; Preparation of nuclear membranes; Isolation of nuclear matrix; Preparation of nucleoids; 5. Isolation of nucleoprotein complexes; Isolation of polynucleosomes of chromatin; Ribonucleoproteins; 6. Isolation of nuclear macromolecules; Isolation of nuclear proteins; Isolation of nuclear RNA; Isolation of DNA; 7. Functional assays of nuclei; Analysis of DNA-binding proteins; Transcription assays; References4. Subcellular fractionation of mitochondria1. Introduction; 2. Purification of mitochondria from various eukaryotic sources; Introduction; Protocols for purification of mitochondria from several eukaryotic sources; Further purification of mitochondrial fractions; 3. Determination of mitochondrial purity; Introduction; Use of the oxygen electrode to determine mitochondrial integrity; Determination of the integrity of the mitochondrial outer membrane; Glucose hexokinase trap method for estimation of P:O ratios; 4. Subfractionation of mitochondriaPreparation of submitochondrial particles by sonicationMany investigations into the structure and function of cells and tissues require the isolation of a particular membrane or subcellular component (organelle). This book covers all the necessary aspects, from breaking up the cells (homogenization), via a variety of separation techniques (the isolation and fractionation chapters), to characterization of the separated organelles.Practical approach series.Subcellular fractionationHandbooks, manuals, etcSubcellular fractionationLaboratory manualsSubcellular fractionationSubcellular fractionation571.6/5Graham J. M(John M.),1943-94317Rickwood D(David)92661MiAaPQMiAaPQMiAaPQBOOK9910780114603321Subcellular fractionation3731851UNINA01119nam0 22002411i 450 UON0018365220231205103143.46288-14-02628-920030730d1990 |0itac50 baitaIT|||| 1||||ˆIl ‰principio del non intervento negli affari internicontributo allo studio de lla tutela giuridica internazionale della potestà di governoRosario Sapienza MilanoGiuffrè1990 - 171 p. ; 22 cm001UON001719122001 Università di Catania. Pubblicazioni della Facoltà di Giurisprudenza 210 Milano Giuffrè126Diritto internazionale pubblicoStudiUONC036685FISAPIENZARosarioUONV1062890ITSOL20250620RICASIBA - SISTEMA BIBLIOTECARIO DI ATENEOUONSIUON00183652SIBA - SISTEMA BIBLIOTECARIO DI ATENEOSI PUB B 0176 SI SC 23584 5 0176 Principio del non intervento negli affari interni62893UNIOR01671nam0 22004333i 450 UFE079397820251003044426.08879593854978887959385420100301d2007 ||||0itac50 baitaitz01i xxxe z01nz01ncRDAcarrieriGeneticaPeter J. Russell2. edNapoliEdiSESc2007XXII, 813 p.ill., diagr., prosp.27 cmAll'ed. it. hanno collaborato: Giorgio Binelli ... [et al.].iGenetics : a molecular approachUSM1274887AQ1V0034541539377GeneticaFIRCFIC004036E572.8Genetica biochimica14572.8GENETICA BIOCHIMICA21QH 431CRussell, Peter J.AQ1V00345407062774Russel, Peter J.CFIV160037Russell, Peter J.Russel, Peter J.CFIV191876Russell, Peter J.Russel, Peter J.CFIV160037Russell, Peter J.Russel, Peter J.CFIV191876Russell, Peter J.ITIT-00000020100301IT-BN0095 IT-NA0079 NAP BNS.MOD La consegna dei documenti è effettuata dall'Ufficio DistribuzioneNAP 01SALA $UFE0793978Biblioteca Centralizzata di Ateneo 01SALA 572.8 RUS ig 0104 0000020425 VMA 1 v.A 2012091820120918 01 BNIGenetics : a molecular approach1539377UNISANNIO