05582nam 2200661 a 450 991077895960332120230725054410.01-283-43385-09786613433855981-4354-86-4(CKB)2550000000079258(EBL)840651(SSID)ssj0000573494(PQKBManifestationID)12207160(PQKBTitleCode)TC0000573494(PQKBWorkID)10543458(PQKB)11427884(MiAaPQ)EBC840651(WSP)00008166(Au-PeEL)EBL840651(CaPaEBR)ebr10524650(CaONFJC)MIL343385(OCoLC)775586481(EXLCZ)99255000000007925820110928d2011 uy 0engur|n|---|||||txtccrScience and technology against microbial pathogens[electronic resource] research, development and evaluation : proceedings of the International Conference on Antimicrobial Research (ICAR2010), Valladolid, Spain, 3-5 November 2010 /editor, A. Mendez-VilasSingapore World Scientificc20111 online resource (447 p.)Description based upon print version of record.981-4354-85-6 Includes bibliographical references.Introduction; Contents; Antimicrobial Peptides; A new class of Scots pine antimicrobial proteins, which act by binding b-glucan Sanjeewani Sooriyaarachchi, Adrian Suárez Covarrubias, Wimal Ubhayasekera, Frederick O. Asiegbu and Sherry L. Mowbray; 1. Introduction; 2. Material and methods; 2.1 Cloning, expression and purification of Sp-AMP3; 2.2 Binding studies; 2.3 Antifungal activity; 2.4 Scanning electron microscopic studies; 2.5 Homology modeling; 3. Results and discussion; 4. Conclusions; ReferencesAntimicrobial aza- -peptides: Structure-activity relationship? B. Legrand, M. Laurencin, C. Zatylny-Gaudin, J. Henry, A. Bondon and M. Baudy Floc'h1. Introduction; 2. Results and Discussion; 3. Conclusion; References; Differential antimicrobial activities of Human Beta-Defensins against Methicillin Resistant (MRSA) and Methicillin sensitive (MSSA) Staphylococcus aureus N. D. S. Herathge, J. T. George and D. A. Rowley; Introduction; Materials and Methods; Results; Discussion; ReferencesIsolation of antimicrobial peptides from New Zealand spinach Tetragonia tetragonioides T. Neubauerová, M. Macková, T. Macek, M. Šanda, M. Králová, I. Doležílková and P. N. Holmsgaard1. General remarks; 2. Materials and methods; 2.1 Extraction of peptides and proteins from spinach leaves; 2.2 Purification of crude extract; 2.3 Reverse-phase chromatography purification (RP-HPLC); 2.4 Microorganisms and antimicrobial assay; 2.5 Antimicrobial activity; 3. Results; 3.1 Isolation of antimicrobial peptides from spinach; 3.2 Antimicrobial assay; 3.3 Characterization by MS; 4. Discussion; ReferencesLysostaphin: molecular changes that preserve staphylolytic activity S. Becker, J. Foster-Frey, A. Powell, H. Mohammadi, D. E. Kerr and D. M. Donovan1. Introduction; 2. Results and Discussion; 2.1 Effect of N- vs. C-terminal 6 x His tag on mLyso lytic activity; 2.2 Activity changes resulting from N125Q and N232Q mLyso mutations; 2.3 N-terminal deletions of mLyso between residue 1 and 31; Conclusions; References; Purification and characterization of antimicrobial peptides from fleshfly larvae haemolymph T. Neubauerová, M. Macková, T. Macek, M. Šanda and Z. Voburka; 1. General remarks2. Materials and methods2.1 Isolation of larval haemolymph; 2.2 Purification of antimicrobial peptides; 2.3 Ion-exchange and reversed-phase liquid chromatography (Ionex-FPLC, RP-HPLC); 2.4 Microorganisms and antimicrobial assay; 2.5 Tricine gel electrophoresis and electroblotting; 2.6 Mass spectrometry analysis; 3. Results and discussion; References; Structural and functional diversity of natural antimicrobial oligopeptides Aexander A. Zamyatnin; 1. Introduction; 2. Structure; 3. Functions; 4. Potential applications; ReferencesThe role of Gram-negative envelope LPS on the bactericidal properties of proteins and peptides: the case of eosinophil cationic protein D. Pulido, M. Torrent, M. V. Nogués and E. BoixThe aim of this book is to disseminate the most recent research in science and technology against microbial pathogens presented at the first edition of the ICAR Conference Series (ICAR2010) held in Valladolid, Spain, in November 2010. This volume is a compilation of 86 chapters written by active researchers that offer information and experiences and afford critical insights into anti-microbe strategies in a general context marked by the threat posed by the increasing antimicrobial resistance of pathogenic microorganisms. "Anti" is here taken in a wide sense as "against cell cycle, adhesion, orDrug resistance in microorganismsCongressesPathogenic bacteriaCongressesDrug resistance in microorganismsPathogenic bacteria579.3165616.9/041Méndez-Vilas A1489100International Conference on Antimicrobial ResearchMiAaPQMiAaPQMiAaPQBOOK9910778959603321Science and technology against microbial pathogens3709640UNINA