04501oam 2200625I 450 991046135410332120200520144314.00-429-16573-01-4398-4832-710.1201/b10919 (CKB)2670000000093650(EBL)726865(OCoLC)730914422(SSID)ssj0000522832(PQKBManifestationID)11320624(PQKBTitleCode)TC0000522832(PQKBWorkID)10538804(PQKB)11536211(MiAaPQ)EBC726865(Au-PeEL)EBL726865(CaPaEBR)ebr10473076(OCoLC)741123114(EXLCZ)99267000000009365020180331d2011 uy 0engur|n|---|||||txtccrGene biotechnology /William Wu. [et al.]3rd ed.Boca Raton :CRC Press,2011.1 online resource (562 p.)A CRC title.1-4398-4830-0 Includes bibliographical references.Front Cover; Contents; Preface; Authors; Chapter 1: Strategies for Novel Research Projects and/or Research Grant Funding; Chapter 2: Rapid Isolation of Specific cDNAs or Genes by PCR; Chapter 3: Construction and Screening of Subtracted and Complete Expression cDNA Libraries; Chapter 4: Subcloning of Genes or DNA Fragments; Chapter 5: Nonisotopic and Isotopic DNA or RNA Sequencing; Chapter 6: Bioinformation Superhighway and Computer Databases of Nucleic Acids and Proteins; Chapter 7: Characterization of DNA or Genes by Southern Blot HybridizationChapter 8: Gene Overexpression by Sense RNA in Mammalian SystemsChapter 9: Gene Underexpression in Cultured Cells and Animals by Antisense DNA and RNA Strategies; Chapter 10: Analysis of Gene Expression at Functional Genomic Level Using Northern Blotting or PCR; Chapter 11: Analysis of Gene Expression at Proteomic Level via Western Blotting; Chapter 12: Analysis of Cellular DNA or Abundance of mRNA by Radioactivity In Situ Hybridization; Chapter 13: Localization of DNA or Abundance of mRNA by Fluorescence In Situ HybridizationChapter 14: In Situ PCR Hybridization of Low Copy Genes and in Situ RT-PCR Detection of Low Abundance mRNAsChapter 15: Isolation and Characterization of Genes from Genomic DNA Libraries; Chapter 16: Mouse Stem Cells as a Model Mammalian Cell Line for Gene Expression; Chapter 17: Strategies for Gene Double Knockout; Chapter 18: Large-Scale Expression and Purification of Recombinant Proteins in Cultured Cells; Chapter 19: Quantitative Analysis of Functional Genome by Real-Time RT-PCR; Chapter 20: High-Throughput Analysis of Gene Expression by Cutting-Edge Technology-DNA Microarrays (Gene Chips)Chapter 21: Construction and Screening of Human Antibody Libraries Using Phage Display TechnologyChapter 22: Down-Regulation of Gene Expression in Mammalian Systems via siRNA Technology; Chapter 23: Strategies for Gene Cloning, Expression, and Identification of Protein-Protein Interaction; Chapter 24: Conditional Gene Knockout; Chapter 25: How to Write a Research Manuscript for Publication in an English Journal; Chapter 26: How to Protect Your Discovery and Invention : Patent 101; Chapter 27: Determination of Transgene Copy Numbers and Practical Biocalculation; Back CoverThe third edition of this popular reference covers a variety of techniques related to gene manipulation, including DNA isolation, preparation, screening, and analysis. Topics range from very basic methods to current and sophisticated technologies, including methodologies created and tested by the authors. Other topics include approaches to grant funding and SiRNA technology. The authors offer detailed, step-by-step explanations of protocols and helpful troubleshooting guides. This edition features new techniques for every chapter, as well as several new chapters--Provided by publisher.Genetic engineeringLaboratory manualsMolecular biologyLaboratory manualsElectronic books.Genetic engineeringMolecular biology660.6/5078Wu William.737012MiAaPQMiAaPQMiAaPQBOOK9910461354103321Gene biotechnology2101266UNINA04991nam 2200661Ia 450 991077749960332120230721031222.01-281-32003-X97866113200340-470-76277-20-470-98753-70-470-98731-6(CKB)1000000000412366(EBL)351362(OCoLC)437218623(SSID)ssj0000225897(PQKBManifestationID)11220442(PQKBTitleCode)TC0000225897(PQKBWorkID)10234565(PQKB)10421719(MiAaPQ)EBC351362(Au-PeEL)EBL351362(CaPaEBR)ebr10236576(CaONFJC)MIL132003(EXLCZ)99100000000041236620060426d2007 uy 0engur|n|---|||||rdacontentrdamediardacarrierPractical pediatric gastrointestinal endoscopy[electronic resource] /George Gershman, Marvin AmentMalden, MA ;Oxford Blackwell Pub.20071 online resource (218 p.)Description based upon print version of record.1-4051-3193-4 Includes bibliographical references and index.Practical Pediatric Gastrointestinal Endoscopy; Contents; Contributors; 1 Introduction; 2 Settings and Staff; The endoscopy unit; Pediatric endoscopy nurse; Disinfections of the endoscopes and accessories; Documentation; Further reading; 3 Equipment; Overview; Insertion tube; Video image capture; "Reading'' the image created on the CCD; Types of CCDs; History of endoscope CCD development; Shape of displayed image; Reproduction of color; Reproduction of motion; Transillumination; Laser therapy with video endoscopes; Image resolution; Color reproduction accuracy; TroubleshootingEndoscope reprocessingSummary; Further reading; 4 Patient Preparation; Pediatric-monitored sedation and anesthesia for diagnostic and therapeutic procedures in endoscopy; During sedation; Postsedation care; Specific sedation techniques; Further reading; 5 Diagnostic Upper Endoscopy Technique; Preparation for esophageal intubation; Assembling the equipment and preprocedure checkup; Endoscope handling; Technique of esophageal intubation; Indications for upper endoscopy; Push enteroscopy/jejunoscopy; Further reading; 6 Therapeutic Upper GI Endoscopy; Benign esophageal stricturePneumatic dilation in achalasiaForeign bodies; Endoscopic hemostasis; Percutaneous endoscopic gastrostomy; Nasojejunal tube placement; Further reading; 7 Pediatric Colonoscopy; Introduction; Indications for colonoscopy; Preparation of the patient for colonoscopy; Equipment; Magnetic imaging system; Informed consent and preprocedure preparation; Sedation for colonoscopy; Embryology of the colon; Endoscopic anatomy; Torque-steering technique; Exploration of the sigmoid colon and sigmoid-descending junction; Splenic flexure and transverse colon; Hepatic flexure, ascending colon, and cecumTerminal ileumComplications; Common pathology; Further reading; 8 Polypectomy; Basic principles of electrosurgery; Snare loops; Polypectomy routine; Safety routine; Safety conditions and techniques; Complications; Further reading; 9 Chromoendoscopy; Indications; Application technique; Recognition of the lesions; Further reading; 10 Wireless Capsule Endoscopy; History; The GIVEN M2A system; Localization; Suspected blood indicator; Indication for use; Diagnostic yield; Contraindications to capsule endoscopy; Pacemaker safety; Bowel preparation; Promotility agents; Endoscopic assistanceComplicationsOutcome of capsule endoscopy; Pediatric patients; Further reading; IndexPractical Pediatric Gastrointestinal Endoscopy is the only text available that provides detailed instructions of all practical aspects of diagnostic and therapeutic endoscopic procedures in children. It gives detailed descriptions of the investigation, diagnosis and therapeutic pediatric GI endoscopy treatments with special attention to the differences between instruments used for adults and for pediatric patients. The advice is based on unique personal experience of thousands of endoscopic or colonoscopic procedures, written by a team of authors with experience in performiPediatric gastroenterologyEndoscopic surgeryGastroenterologyEndoscopic surgeryPediatric gastroenterologyEndoscopic surgery.GastroenterologyEndoscopic surgery.618.92/3307545618.9263307545Gershman George923193Ament Marvin Earl1938-923195MiAaPQMiAaPQMiAaPQBOOK9910777499603321Practical pediatric gastrointestinal endoscopy2254155UNINA03187nam 2200625 a 450 991096008240332120251117091437.00-8232-4125-40-8232-5086-5(CKB)2550000000054744(EBL)3239590(OCoLC)763156431(SSID)ssj0000628073(PQKBManifestationID)11401336(PQKBTitleCode)TC0000628073(PQKBWorkID)10701874(PQKB)10281821(StDuBDS)EDZ0000054495(Au-PeEL)EBL3239590(CaPaEBR)ebr10497676(MiAaPQ)EBC3239590(EXLCZ)99255000000005474420110302d2011 uy 0engurcn|||||||||txtccrBetter off dead the evolution of the zombie as post-human /edited by Deborah Christie and Sarah Juliet Lauro1st ed.New York Fordham University Press20111 online resource (304 p.)0-8232-3446-0 Includes bibliographical references and index.Copyright ; Contents; Better Off Dead; And the Dead Shall Rise; ''They are not men . . . they are dead bodies!'': From Cannibal to Zombie and Back Again; ''We are the mirror of your fears'': Haitian Identity and Zombification; Undead Radio: Zombies and the Living Dead on 1930's and 1940's Radio Drama; The Zombie as Other: Mortality and the Monstrous in the Post-Nuclear Age; And the Dead Shall Walk; A Dead New World: Richard Matheson and the Modern Zombie; Nuclear Death and Radical Hope in Dawn of the Dead and On the Beach; Lucio Fulci and the Decaying Definition of Zombie NarrativesImitations of Life: Zombies and the Suburban Gothic All Dark Inside: Dehumanization and Zombification in Postmodern Cinema; And the Dead Shall Inherit the Earth; Slacker Bites Back: Shaun of the Dead Finds New Life for Deadbeats; Zombie Movies and the ''Millennial Generation''; ''Off the page and into your brains!'': New Millennium Zombies and the Scourge of Hopeful Apocalypses; Playing Dead: Zombies Invade Performance Art . . . and Your Neighborhood; Selected Biography ; Contributors ; IndexFrom comic books to video games, to internet applications & homemade films, zombies are all around us. Investigating the zombie from an interdisciplinary perspective, with an emphasis on deep analytical engagement with diverse kinds of texts, this volume addresses some of the more unlikely venues where zombies are found.ZombiesZombies in popular cultureZombies in literatureZombies in motion picturesZombies.Zombies in popular culture.Zombies in literature.Zombies in motion pictures.398.21Christie Deborah1891929Lauro Sarah Juliet886318MiAaPQMiAaPQMiAaPQBOOK9910960082403321Better off dead4536367UNINA