00750nam0-2200253 --450 991077628100332120240129115706.020240129d1920----kmuy0itay5050 bafreFR 001yy<<La >>pomme de terreconseils pratiques pour améliorer sa culturepar S. MottetParisLibraire Agricole de la Maison Rustique192072 p.ill.25 cmPatataColtivazione211.84itaMottet,Séraphin Joseph1585946ITUNINAREICATUNIMARCBK9910776281003321A PAT 707434/2024FAGBCFAGBCPomme de terre3871887UNINA03809nam 22007095 450 991025402970332120200630070650.03-319-32723-210.1007/978-3-319-32723-5(CKB)3710000000653174(EBL)4515774(SSID)ssj0001665730(PQKBManifestationID)16455710(PQKBTitleCode)TC0001665730(PQKBWorkID)15000233(PQKB)11530661(DE-He213)978-3-319-32723-5(MiAaPQ)EBC4515774(PPN)193445018(EXLCZ)99371000000065317420160427d2016 u| 0engur|n|---|||||txtccrUsing Mass Spectrometry for Biochemical Studies on Enzymatic Domains from Polyketide Synthases /by Matthew Jenner1st ed. 2016.Cham :Springer International Publishing :Imprint: Springer,2016.1 online resource (189 p.)Springer Theses, Recognizing Outstanding Ph.D. Research,2190-5053Description based upon print version of record.3-319-32722-4 Includes bibliographical references at the end of each chapters.Introduction -- Materials and Methods -- Substrate Specificity of Ketosynthase Domains Part I: β-Branched Acyl Chains -- Substrate Specificity of Ketosynthase Domains Part II: Amino Acid-Containing Acyl Chains -- Synthesis of Acyl-Acyl Carrier Proteins and their use in Studying Polyketide Synthase Enzymology -- Substrate Specificity of Ketosynthase Domains Part III: Elongation-Based Substrate Specificity.This thesis reports studies on the substrate specificity of crucial ketosynthase (KS) domains from trans-AT Polyketide Synthases (PKSs). Using a combination of electrospray ionisation-mass spectrometry (ESI-MS) and simple N-acetyl cysteamine (SNAC) substrate mimics, Matthew Jenner has successfully studied the specificity of a range of KS domains from the bacillaene and psymberin PKSs with regard to the initial acylation step of KS-catalysis. The findings in this thesis provide important insights into mechanisms of KS specificity and show that mutagenesis can be used to expand the repertoire of acceptable substrates for future PKS engineering. The documentation of this research is a useful reference and guideline for students starting a PhD in this field.Springer Theses, Recognizing Outstanding Ph.D. Research,2190-5053Mass spectrometryEnzymologyBiochemical engineeringMedical biochemistryMass Spectrometryhttps://scigraph.springernature.com/ontologies/product-market-codes/C11010Enzymologyhttps://scigraph.springernature.com/ontologies/product-market-codes/L14070Biochemical Engineeringhttps://scigraph.springernature.com/ontologies/product-market-codes/C12029Medical Biochemistryhttps://scigraph.springernature.com/ontologies/product-market-codes/H35005Mass spectrometry.Enzymology.Biochemical engineering.Medical biochemistry.Mass Spectrometry.Enzymology.Biochemical Engineering.Medical Biochemistry.572.45Jenner Matthewauthttp://id.loc.gov/vocabulary/relators/aut1059892MiAaPQMiAaPQMiAaPQBOOK9910254029703321Using Mass Spectrometry for Biochemical Studies on Enzymatic Domains from Polyketide Synthases2509383UNINA