03662nam 22006255 450 991063109350332120251009102811.09783031108365(electronic bk.)978303110835810.1007/978-3-031-10836-5(MiAaPQ)EBC7141577(Au-PeEL)EBL7141577(CKB)25360918100041(OCoLC)1351200448(PPN)266355021(DE-He213)978-3-031-10836-5(EXLCZ)992536091810004120221116d2022 u| 0engurcnu||||||||txtrdacontentcrdamediacrrdacarrierFlow Cytometry Today Everything You Need to Know about Flow Cytometry /by Claudio Ortolani1st ed. 2022.Cham :Springer International Publishing :Imprint: Springer,2022.1 online resource (556 pages)Biomedical and Life Sciences SeriesPrint version: Ortolani, Claudio Flow Cytometry Today Cham : Springer International Publishing AG,c2022 9783031108358 Includes bibliographical references and index.Chapter 1. General Principles -- Chapter 2. Signals – Scattering -- Chapter 3. Signals - Fluorescence, Phosphorescence, Impedance, Extinction -- Chapter 4. Fluidics -- Chapter 5. Light Sources -- Chapter 6. Optical Benches -- Chapter 7. Detectors and Electronics -- Chapter 8. Signal Analysis -- Chapter 9. The Cytometric File -- Chapter 10. Data Transformation -- Chapter 11. Data Representation -- Chapter 12. Data Analysis -- Chapter 13. Standards, Setup, Calibration, and Control Techniques -- Chapter 14. Fluorochromes – Overview -- Chapter 15. Fluorochromes Suitable for Antibody Conjugation -- Chapter 16. Fluorochromes That Bind Nucleic Acids -- Chapter 17. Fluorochromes for the Study of the Cell Features -- Chapter 18. Fluorescent Proteins -- Chapter 19. Spillover and Compensation -- Chapter 20. Artifacts -- Chapter 21. Cell Sorting -- Chapter 22. Non-Conventional Flow Cytometry -- Chapter 23. Statistics: A Cytometric Point of View.This book covers all the technical aspects of flow cytometry needed to set-up the instrument, solve problems encountered in daily work, or necessary for exam preparation. It provides the reader with an in-depth look at the device and its applications. Each component and its function is described in an easy-to-understand manner, giving the reader a sound basic knowledge of this instrument. The practical examples given, simplify and enhance the learning process. This book is a unique resource of knowledge for biomedical engineers and biotechnologists, flow cytometry operators, laboratory technicians and biomedical researchers, both biologists as well as medical doctors, and can also be a helpful tool for companies and manufacturers.Biomedical and Life Sciences SeriesCytologyBiologyTechniqueMedicineResearchBiologyResearchCell BiologyBiological TechniquesBiomedical ResearchCytology.BiologyTechnique.MedicineResearch.BiologyResearch.Cell Biology.Biological Techniques.Biomedical Research.910.5Ortolani C(Claudio),292311MiAaPQMiAaPQMiAaPQ9910631093503321Flow Cytometry Today2965719UNINA