04425nam 2201081z- 450 991055750230332120231214133036.0(CKB)5400000000044533(oapen)https://directory.doabooks.org/handle/20.500.12854/68280(EXLCZ)99540000000004453320202105d2021 |y 0engurmn|---annantxtrdacontentcrdamediacrrdacarrier3D Stem Cell CultureBasel, SwitzerlandMDPI - Multidisciplinary Digital Publishing Institute20211 electronic resource (186 p.)3-03943-803-4 3-03943-804-2 Recently, stem cells have been drawing increasing interest in basic and translational research that aims to understand stem cell biology and generate new therapies for various disorders. Many stem cells can be cultured in 2D relatively easily using tissue culture plastic. However, many of these cultures do not represent the natural conditions of stem cells in the body. In the body, microenvironments include numerous supporting cells and molecules. Therefore, researchers and clinicians have sought ideal stem cell preparations for basic research and clinical applications, which may be attainable through 3D culture of stem cells. The 3D cultures mimic the conditions of the natural environment of stem cells better, as cells in 3D cultures exhibit many unique and desirable characteristics that could be beneficial for therapeutic interventions. 3D stem cell cultures may employ supporting structures, such as various matrices or scaffolds, in addition to stem cells, to support complex structures. This book brings together recent research on 3D cultures of various stem cells to increase the basic understanding of stem cell culture techniques and also to highlight stem cell preparations for possible novel therapeutic applications.Research & information: generalbicsscBiology, life sciencesbicsschematopoiesishematopoietic stem cellsstem cell culture2D culture3D cultureembryonic stem cellsthree-dimensionalself-assembling scaffoldpluripotencyculture conditionsexpansiongrowthnichehuman cortical progenitorssilicon pillarscell growthhiPSC-derived neural progenitorscerebral cortexcarcinogenprotein phosphatase 2A (PP2A)intestinal tumorintestinal organoidLgr5+ crypt stem cellmouse embryonic stem celldifferentiation protocolureteric bud progenitor cells3D kidney organoidsintestinal organoidscanine intestinedifferentiationorganoid cultureinduced pluripotent stem cellsneurospheresneurite outgrowthneurotoxicityhBM-MSCscytokinestenogenic markerscyclic strain3D microenvironmentPLGA carriersbioreactorcardiac microtissuesiPSC-derived cardiomyocytescardiac fibroblastscardiac fibrosiscardiac rhythmTGF-β signallingdrug screeningin vitro modelstem cell3Dculture conditionregenerative medicinescaffoldorganoidadipose tissue-derived mesenchymal stem cellsstromal vascular fractionplatelet rich plasmaplatelet concentratesveterinary regenerative medicineResearch & information: generalBiology, life sciencesYlostalo Joni Hedt1289237Ylostalo Joni HothBOOK99105575023033213D Stem Cell Culture3021124UNINA