02258nam 2200433 450 991055527350332120180321083429.01-118-69681-61-118-69674-31-118-69673-5(CKB)4330000000006821(MiAaPQ)EBC5724036(OCoLC)1089445751(EXLCZ)99433000000000682120190322d2019 uy 0engurcnu||||||||txtrdacontentcrdamediacrrdacarrierUnderstanding light microscopy /by Jeremy SandersonHoboken, NJ :Wiley,2019.1 online resource (850 pages)Royal Microscopical Society/John Wiley Series0-470-97375-7 Includes bibliographical references and index.Our eyes and the microscope -- Light -- Basic microscope optics -- Microscope design -- Ergonomics -- Optical aberrations of the microscope -- The microscope objective -- Condensers & eyepieces -- Illumination in the microscope -- Diffraction and image formation in microscopy -- Contrast generation & enhancement -- Reflected-light microscopy -- Polarised-light microscopy : part 1, theory -- Polarised-light microscopy : part 2, applied -- Fluorescence microscopy -- Fluorophores & fluorescent proteins -- Optical sectioning & confocal microscopy -- Operating the confocal microscope -- Light-sheet microscopy -- Bleed-through & spectral unmixing -- Deconvolution -- Multi-photon microscopy -- Total internal reflection fluorescence microscopy -- Frap and fret -- Colocalisation -- Super-resolution fluorescence microscopy -- Choosing a microscope platform and core imaging facilities -- Biological specimen preparation -- Materials specimen preparation -- Recording the image : part 1, theory -- Recording the image : part 2, applied.RMS-Wiley series.MicroscopyMicroscopy.570.28/2Sanderson J. B(Jeremy B.),1218598MiAaPQMiAaPQMiAaPQBOOK9910555273503321Understanding light microscopy2818093UNINA