02176nam 2200649 450 991046531750332120200520144314.00-8229-5969-00-8229-7328-6(CKB)3710000000221944(OCoLC)647182099(CaPaEBR)ebrary10908930(SSID)ssj0001334274(PQKBManifestationID)11739869(PQKBTitleCode)TC0001334274(PQKBWorkID)11405947(PQKB)11634276(MiAaPQ)EBC2038806(MdBmJHUP)muse32109(Au-PeEL)EBL2038806(CaPaEBR)ebr10908930(CaONFJC)MIL635885(EXLCZ)99371000000022194420140829h20052005 uy 0engurcnu||||||||txtccr(Re)Writing craft composition, creative writing, and the future of English studies /Tim MayersPittsburgh, Pennsylvania :University of Pittsburgh Press,2005.©20051 online resource (209 p.) Pittsburgh Series in Composition, Literacy, and CultureBibliographic Level Mode of Issuance: Monograph1-322-04634-4 0-8229-4256-9 Includes bibliographical references and index.Pittsburgh series in composition, literacy, and culture.English languageRhetoricStudy and teachingReport writingStudy and teaching (Higher)English philologyStudy and teachingCreative writing (Higher education)Electronic books.English languageRhetoricStudy and teaching.Report writingStudy and teaching (Higher)English philologyStudy and teaching.Creative writing (Higher education)808/.042/071Mayers Tim1039905MiAaPQMiAaPQMiAaPQBOOK9910465317503321Re)Writing craft2462384UNINA05731nam 2200733Ia 450 991013959360332120200520144314.01-283-28285-297866132828591-118-13703-51-118-13704-31-118-13701-9(CKB)2550000000054371(EBL)818767(OCoLC)761321884(SSID)ssj0000540463(PQKBManifestationID)11324647(PQKBTitleCode)TC0000540463(PQKBWorkID)10598118(PQKB)11093674(MiAaPQ)EBC818767(Au-PeEL)EBL818767(CaPaEBR)ebr10501256(CaONFJC)MIL328285(PPN)171925513(EXLCZ)99255000000005437120110601d2012 uy 0engur|n|---|||||txtccrProteomics of biological systems[electronic resource] protein phosphorylation using mass spectrometry techniques /Bryan M HamHoboken, N.J. John Wiley & Sons20121 online resource (376 p.)Description based upon print version of record.1-118-02896-1 Includes bibliographical references and index.PROTEOMICS OF BIOLOGICAL SYSTEMS: Protein Phosphorylation Using Mass Spectrometry Techniques; CONTENTS; PREFACE; ACKNOWLEDGMENTS; ABOUT THE AUTHOR; 1: Posttranslational Modification (PTM) of Proteins; 1.1 OVER 200 FORMS OF PTM OF PROTEINS; 1.2 THREE MAIN TYPES OF PTM STUDIED BY MS; 1.3 OVERVIEW OF NANO-ELECTROSPRAY/NANOFLOW LC-MS; 1.3.1 Definition and Description of MS; 1.3.2 Basic Design of Mass Analyzer Instrumentation; 1.3.3 ESI; 1.3.4 Nano-ESI; 1.4 OVERVIEW OF NUCLEIC ACIDS; 1.5 PROTEINS AND PROTEOMICS; 1.5.1 Introduction to Proteomics; 1.5.2 Protein Structure and Chemistry1.5.3 Bottom-Up Proteomics: MS of Peptides1.5.3.1 History and Strategy; 1.5.3.2 Protein Identification through Product Ion Spectra; 1.5.3.3 High-Energy Product Ions; 1.5.3.4 De Novo Sequencing; 1.5.3.5 Electron Capture Dissociation (ECD); 1.5.4 Top-Down Proteomics: MS of Intact Proteins; 1.5.4.1 Background; 1.5.4.2 GP Basicity and Protein Charging; 1.5.4.3 Calculation of Charge State and Molecular Weight; 1.5.4.4 Top-Down Protein Sequencing; 1.5.5 Systems Biology and Bioinformatics; 1.5.6 Biomarkers in Cancer; REFERENCES; 2: Glycosylation of Proteins; 2.1 PRODUCTION OF A GLYCOPROTEIN2.2 BIOLOGICAL PROCESSES OF PROTEIN GLYCOSYLATION2.3 N-LINKED AND O-LINKED GLYCOSYLATION; 2.4 CARBOHYDRATES; 2.4.1 Ionization of Oligosaccharides; 2.4.2 Carbohydrate Fragmentation; 2.4.3 Complex Oligosaccharide Structural Elucidation; 2.5 THREE OBJECTIVES IN STUDYING GLYCOPROTEINS; 2.6 GLYCOSYLATION STUDY APPROACHES; 2.6.1 MS of Glycopeptides; 2.6.2 Mass Pattern Recognition; 2.6.2.1 High Galactose Glycosylation Pattern; 2.6.3 Charge State Determination; 2.6.4 Diagnostic Fragment Ions; 2.6.5 High-Resolution/High-Mass Accuracy Measurement and Identification; 2.6.6 Digested Bovine FetuinREFERENCES3: Sulfation of Proteins as Posttranslational Modification; 3.1 GLYCOSAMINOGLYCAN SULFATION; 3.2 CELLULAR PROCESSES INVOLVED IN SULFATION; 3.3 BRIEF EXAMPLE OF PHOSPHORYLATION; 3.4 SULFOTRANSFERASE CLASS OF ENZYMES; 3.5 FRAGMENTATION NOMENCLATURE FOR CARBOHYDRATES; 3.6 SULFATED MUCIN OLIGOSACCHARIDES; 3.7 TYROSINE SULFATION; 3.8 TYROSYLPROTEIN SULFOTRANSFERASES TPST1 AND TPST2; 3.9 O-SULFATED HUMAN PROTEINS; 3.10 SULFATED PEPTIDE PRODUCT ION SPECTRA; 3.11 USE OF HIGHER ENERGY COLLISIONS; 3.12 ELECTRON CAPTURE DISSOCIATION (ECD); 3.13 SULFATION VERSUS PHOSPHORYLATION; REFERENCES4: Eukaryote PTM as Phosphorylation: Normal State Studies4.1 MASS SPECTRAL MEASUREMENT WITH EXAMPLES OF HELA CELL PHOSPHOPROTEOME; 4.1.1 Introduction; 4.1.2 Protein Phosphatase and Kinase; 4.1.3 Hydroxy-Amino Acid Phosphorylation; 4.1.4 Traditional Phosphoproteomic Approaches; 4.1.5 Current Approaches; 4.1.5.1 Phosphoproteomic Enrichment Techniques; 4.1.5.2 IMAC; 4.1.5.3 MOAC; 4.1.5.4 Methylation of Peptides prior to IMAC or MOAC Enrichment; 4.1.6 The Ideal Approach; 4.1.7 One-Dimensional (1-D) Sodium Dodecyl Sulfate (SDS) PAGE; 4.1.8 Tandem MS Approach; 4.1.8.1 pS Loss of Phosphate Group4.1.8.2 pT Loss of Phosphate GroupPhosphorylation is the addition of a phosphate (PO4) group to a protein or other organic molecule. Phosphorylation activates or deactivates many protein enzymes, causing or preventing the mechanisms of diseases such as cancer and diabetes. This book shows how to use mass spectrometry to determine whether or not a protein has been correctly modified by the addition of a phosphate group. It also provides a combination of detailed, step-by-step methodology for phosphoproteomic sample preparation, mass spectral instrumental analysis, and data interpretation approaches. Furthermore, it iProteomicsMethodologyPhosphorylationResearchMethodologyPhosphoproteinsSynthesisMass spectrometryBiological systemsResearchMethodologyProteomicsMethodology.PhosphorylationResearchMethodology.PhosphoproteinsSynthesis.Mass spectrometry.Biological systemsResearchMethodology.572/.62Ham Bryan M314951MiAaPQMiAaPQMiAaPQBOOK9910139593603321Proteomics of biological systems2104474UNINA00848cam0-2200313---450 99000555510040332120230922145132.00521222354000555510FED01000555510(Aleph)000555510FED0119990604d1983----km-y0itay50------baengGBy-------001yyPragmaticsStephen C. LevinsonCambridgeCambridge University Pressc1983XVI, 420 p.22 cmCambridge textbooks in linguisticsLinguisticaPragmaticaLevinson,Stephen C.157418ITUNINARICAUNIMARCBK990005555100403321401.45 LEV 1BIBL. 56556FLFBCFLFBCPragmatics51558UNINA01005cam0 22002771 450 SOBE0008402120250716085757.020250716d1967 |||||ita|0103 baitaIT<<Il >>Futurismo. Parte secondatesto di Maurizio CalvesiMilanoFabbri1967P. 120-192ill.32 cm<L'>arte moderna[direttore Franco Russoli]14001SOBE000840082001 L'*arte moderna / [direttore Franco Russoli]14<<Il >>FuturismoSOBA00034938299636Calvesi, Maurizio <1927-2020>AF0000982307036175ITUNISOB20250716RICAUNISOBUNISOBFondo|Calì183789SOBE00084021M 102 Monografia moderna SBNMFondo|Calì001396SI18378920250520CaliDonoIrovitoUNISOBUNISOB20250716085616.020250716085651.0rovitoFuturismo299636UNISOB