05277nam 2200709 450 991045992800332120200520144314.00-19-756276-00-19-021374-41-68015-865-10-19-021373-6(CKB)3710000000329346(EBL)1911965(MiAaPQ)EBC1911965(StDuBDS)EDZ0002341503(Au-PeEL)EBL1911965(CaPaEBR)ebr11000834(CaONFJC)MIL688367(OCoLC)899942202(EXLCZ)99371000000032934620150115h20152015 uy 0engur|n|---|||||rdacontentrdamediardacarrierBioseparations science and engineering /Roger G. Harrison [and three others]Second edition.New York :Oxford University Press,2015.©20151 online resource (577 p.)Topics in Chemical Engineering : A Series of Textbooks and MonographsThis edition previously issued in print: 2015.1-322-57085-X 0-19-539181-0 Includes bibliographical references at the end of each chapters and index.Cover; Bioseparations Science and Engineering; Copyright; Dedication; Contents; Preface; 1 Introduction to Bioproducts and Bioseparations; 1.1 Instructional Objectives; 1.2 Broad Classification of Bioproducts; 1.3 Small Biomolecules; 1.3.1 Primary Metabolites; 1.3.2 Secondary Metabolites; 1.3.3 Summary of Small Biomolecules; 1.4 Macromolecules: Proteins; 1.4.1 Primary Structure; 1.4.2 Secondary Structure; 1.4.3 Tertiary Structure; Example 1.1 Effect of a Reducing Agent on Protein Structure and Mobility; 1.4.4 Quaternary Structure; 1.4.5 Prosthetic Groups and Hybrid Molecules1.4.6 Functions and Commercial Uses of Proteins1.4.7 Stability of Proteins; 1.4.8 Recombinant Protein Expression; 1.5 Macromolecules: Nucleic Acids and Oligonucleotides; 1.6 Macromolecules: Polysaccharides; 1.7 Particulate Products; 1.8 Introduction to Bioseparations: Engineering Analysis; 1.8.1 Stages of Downstream Processing; Example 1.2 Initial Selection of Purification Steps; 1.8.2 Basic Principles of Engineering Analysis; 1.8.3 Process and Product Quality; 1.8.4 Criteria for Process Development; 1.9 The Route to Market; 1.9.1 The Chemical and Applications Range of the Bioproduct1.9.2 Documentation of Pharmaceutical Bioproducts1.9.3 GLP and cGMP; 1.9.4 Formulation; 1.10 Summary; Nomenclature; Problems; References; 2 Analytical Methods and Bench Scale Preparative Bioseparations; 2.1 Instructional Objectives; 2.2 Specifications; 2.3 Assay Attributes; 2.3.1 Precision; 2.3.2 Accuracy; 2.3.3 Specificity; 2.3.4 Linearity, Limit of Detection, and Limit of Quantitation; 2.3.5 Range; 2.3.6 Robustness; 2.4 Analysis of Biological Activity; 2.4.1 Animal Model Assays; 2.4.2 Cell-Line-Derived Bioassays; 2.4.3 In vitro Biochemical AssaysExample 2.1 Coupled Enzyme Assay for Alcohol Oxidase2.5 Analysis of Purity; 2.5.1 Electrophoretic Analysis; Example 2.2 Estimation of the Maximum Temperature in an Electrophoresis Gel; 2.5.2 High-Performance Liquid Chromatography (HPLC); 2.5.3 Mass Spectrometry; 2.5.4 Coupling of HPLC with Mass Spectrometry; 2.5.5 Ultraviolet Absorbance; Example 2.3 Determination of Molar Absorptivity; 2.5.6 CHNO/Amino Acid Analysis (AAA); Example 2.4 Calculations Based on CHNO Analysis; 2.5.7 Protein Assays; 2.5.8 Enzyme-Linked Immunosorbent Assay; 2.5.9 Gas Chromatography; 2.5.10 DNA Hybridization2.5.11 ICP/MS (AES)2.5.12 Dry Weight; 2.6 Microbiology Assays; 2.6.1 Sterility; 2.6.2 Bioburden; 2.6.3 Endotoxin; 2.6.4 Virus, Mycoplasma, and Phage; 2.7 Bench Scale Preparative Separations; 2.7.1 Preparative Electrophoresis; 2.7.2 Magnetic Bioseparations; 2.8 Summary; Nomenclature; Problems; References; 3 Cell Lysis and Flocculation; 3.1 Instructional Objectives; 3.2 Some Elements of Cell Structure; 3.2.1 Prokaryotic Cells; 3.2.2 Eukaryotic Cells; 3.3 Cell Lysis; 3.3.1 Osmotic and Chemical Cell Lysis; 3.3.2 Mechanical Methods of Lysis; 3.4 Flocculation; 3.4.1 The Electric Double LayerExample 3.1 Dependence of the Debye Radius on the Type of ElectrolyteAn updated edition of a comprehensive and authoritative chemical engineering textbook on bioseparations science, updated to include new information on topics like moment analysis, chromatography, and evaporation.Topics in chemical engineering (Oxford University Press)BiomoleculesSeparationBiochemical engineeringSeparation (Technology)Extraction (Chemistry)Electronic books.BiomoleculesSeparation.Biochemical engineering.Separation (Technology)Extraction (Chemistry)660/.2842Harrison Roger G.1944-MiAaPQMiAaPQMiAaPQBOOK9910459928003321Bioseparations science and engineering2015915UNINA02660nam 2200613Ia 450 991078871220332120230725050451.01-282-65086-697866126508641-4438-2191-8(CKB)3390000000009235(EBL)1133087(OCoLC)830168251(SSID)ssj0000436879(PQKBManifestationID)11311444(PQKBTitleCode)TC0000436879(PQKBWorkID)10428871(PQKB)11603257(MiAaPQ)EBC1133087(Au-PeEL)EBL1133087(CaPaEBR)ebr10677027(CaONFJC)MIL265086(OCoLC)816564759(FINmELB)ELB132212(EXLCZ)99339000000000923520100923d2010 uy 0engur|n|---|||||txtccrFrom question to quest[electronic resource] literary-philosophical enquiries into the challenges of life /by Marian F. Sia and Santiago SiaNewcastle upon Tyne, UK Cambridge Scholars Pub.2010Newcastle upon Tyne, UK :Cambridge Scholars Pub.,2010.1 online resource (254 p.)Description based upon print version of record.1-4438-2159-4 Includes bibliographical references (p. [237]-242).OTHER BOOKS; TABLE OF CONTENTS; FOREWORD; PREFACE; INTRODUCTION; CHAPTER ONE; CHAPTER TWO; CHAPTER THREE; CHAPTER FOUR; CHAPTER FIVE; CHAPTER SIX; CHAPTER SEVEN; CHAPTER EIGHT; POSTSCRIPT; APPENDIX A; APPENDIX B; SELECTED BIBLIOGRAPHYIn facing up to life and its challenges, questions inevitably arise. Different situations provoke specific questions-mostly trivial but frequently fundamental-always seeking some kind of answer. While the transition from question to quest is a rather natural one for human beings and the need for answers is a serious human demand, the quest itself is significant, precisely because it is a human task. This book offers a number of literary-philosophical enquiries into these challenges of life. ...LiteraturePhilosophyLiteratureHistory and criticismTheory, etcLiteraturePhilosophy.LiteratureHistory and criticismTheory, etc.801Sia Marian F922313Sia Santiago1470429MiAaPQMiAaPQMiAaPQBOOK9910788712203321From question to quest3831546UNINA