06109nam 2200697 450 991045922200332120200520144314.01-60807-012-3(CKB)2670000000044609(EBL)946536(OCoLC)796382978(SSID)ssj0000423085(PQKBManifestationID)11281847(PQKBTitleCode)TC0000423085(PQKBWorkID)10440496(PQKB)11766329(MiAaPQ)EBC946536(Au-PeEL)EBL946536(CaPaEBR)ebr10412731(CaBNVSL)mat09100412(IEEE)9100412(EXLCZ)99267000000004460920200729d2010 uy engur|n|---|||||txtccrMethods in bioengineering alternatives to animal testing /Tim Maguire, Eric Novik, editorsBoston, Massachusetts :Artech House,©2010.[Piscataqay, New Jersey] :IEEE Xplore,[2010]1 online resource (298 p.)Methods in bioengineering seriesDescription based upon print version of record.1-60807-011-5 Includes bibliographical references and index.Contents; Preface; Chapter 1 Current Methods for Prediction of Human Hepatic Clearance Using In Vitro Intrinsic Clearance; 1.1 Introduction; 1.2 Materials; 1.3 Methods; 1.3.1 Thawing the hepatocytes; 1.3.2 Clearance study using a hepatocyte suspension; 1.3.3 Clearance study using a plated hepatocyte culture; 1.3.4 Clearance study using a plated hepatocyte culture under a flow condition; 1.3.5 Sampling for the clearance study; 1.3.6 Sample analysis using LC-MS/MS; 1.4 Data Acquisition, Anticipated Results, and Interpretation; 1.4.1 Hepatocyte suspension and plated hepatocyte system1.4.2 Physiologically based microfluidic systems1.5 Discussion and Commentary; 1.5.1 Hepatocyte suspension system; 1.5.2 Plated hepatocyte system; 1.5.3 Physiologically based microfluidic systems; 1.6 Summary; References; Chapter 2 Use of Permeability from Cultured Cell Linesand PAMPA System and Absorption from Experimental Animals for the Prediction of Absorption in Humans; 2.1 Introduction; 2.2 Materials; 2.3 Methods; 2.3.1 Cultured cell system; 2.3.2 PAMPA system; 2.3.3 In vivo absorption measurements; 2.4 Data Acquisition, Anticipated Results, and Interpretation; 2.4.1 Data analysis2.4.2 Results and interpretation2.5 Discussion and Commentary; 2.5.1 Cell culture and PAMPA systems; 2.5.2 Absorption in experimental animals; 2.5.3 Rats; 2.5.4 Dogs; 2.5.5 Monkeys; 2.6 Summary; References; Chapter 3 Aggregating Brain Cell Cultures for Neurotoxicity Tests; 3.1 Introduction; 3.2 Experimental Design; 3.3 Materials; 3.3.1 Animals; 3.3.2 Special equipment; 3.3.3 Reagents; 3.3.4 Preparation of solutions and media; 3.4 Methods; 3.4.1 Washing and sterilizing the glassware; 3.4.2 Cell isolation and culture preparation3.4.3 Maintenance of aggregating brain cell cultures (media replenishmentand subdivision)3.4.4 Preparation and treatment of replicate cultures; 3.4.5 Harvest of replicate cultures for various analytical procedures; 3.4.6 Examples of sample preparation and use for various analytical procedures; 3.4.7 Data Analysis; 3.5 Anticipated Results; 3.6 Discussion and Commentary; 3.7 Application Notes; 3.8 Summary Points; Acknowledgments; References; Chapter 4 Approaches Towards a Multiscale Model of Systemic Inflammation in Humans; 4.1 Introduction; 4.2 Materials4.2.1 Human endotoxin model and data collection4.3 Methods; 4.3.1 Transcriptional dynamics and intrinsic responses; 4.3.2 Modeling inflammation at the cellular level; 4.3.3 Modeling inflammation at the systemic level; 4.4 Results; 4.4.1 Elements of the multiscale host response model of human inflammation; 4.4.2 Estimation of relevant model parameters; 4.4.3 Qualitative assessment of the model; 4.5 Conclusions; Acknowledgments; References; Chapter 5 A Liposome Assay for Evaluating the Ocular Toxicity of Chemicals; 5.1 Introduction; 5.2 Experimental Design; 5.3 Materials; 5.4 Methods5.4.1 Preparation of calcein-loaded liposomesWritten and edited by recognized experts in the field, the new Artech House Methods in Bioengineering book series offers detailed guidance on authoritative methods for addressing specific bioengineering challenges. Offering a highly practical presentation of each topic, each book provides research engineers, scientists, and students with step-by-step procedures, clear examples, and effective ways to overcome problems that may be encountered. Providing alternatives to animal testing is one of the hottest topics in biomedical research, and this groundbreaking volume addresses this critical issues head on. This unique book presents techniques and methods at the forefront of scientific research that have the potential to replace certain whole animal tests. Moreover, this book provides a platform where other widely accepted techniques and scientific advancements can be collated into a concise set of methods that can be implemented within both academic and industrial communities.Publisher abstract.Artech House methods in bioengineering series.Animal experimentationMoral and ethical aspectsAnimal welfareMoral and ethical aspectsHuman-animal relationshipsMoral and ethical aspectsElectronic books.Animal experimentationMoral and ethical aspects.Animal welfareMoral and ethical aspects.Human-animal relationshipsMoral and ethical aspects.179.4Maguire Timothy J(Timothy John)988743Novik Eric988744CaBNVSLCaBNVSLCaBNVSLBOOK9910459222003321Methods in bioengineering2260798UNINA01846oam 2200565 450 991071548300332120210609093601.0(CKB)5470000002511211(OCoLC)755257644(OCoLC)1084521641(EXLCZ)99547000000251121120111002d1934 ua 0engur|||||||||||txtrdacontentcrdamediacrrdacarrierRoadside improvementWashington, D.C. :United States Department of Agriculture,1934.1 online resource (34 pages) illustrationsMiscellaneous publication / United States Department of Agriculture ;no. 191"By Wilbur H. Simonson and R.E. Royall"--Page 1.Publication pre-dates Federal Depository Library Program (FDLP) item numbers. No FDLP item number has been assigned.Includes bibliographical references (pages 32-35).Roadside improvementUnited StatesHighway engineeringUnited StatesHighway engineeringfastRoadside improvementfastUnited StatesfastRoadside improvementHighway engineeringHighway engineering.Roadside improvement.Simonson Wilbur H(Wilbur Herbert),1897-1414996Royall R. E.United States.Department of Agriculture.OCLCEOCLCEOCLCQOCLCOOCLCAOCLCQOCLCFOCLCQOCLCAINARCGPOBOOK9910715483003321Roadside improvement3515826UNINA01807nam 2200541Ia 450 991069726140332120230902161553.0(CKB)5470000002386307(OCoLC)646194592(EXLCZ)99547000000238630720100707d1996 ua 0engurbn|||||||||txtrdacontentcrdamediacrrdacarrierIncreased efficiency LED[electronic resource] /inventors, Claudio Oliviera Egalon, Robert S. Rogowski[Redacted ed.][Washington, DC] :[National Aeronautics and Space Administration],[1996]1 online resource illustrationsNASA case ;no. LAR 15184-1-SBTitle from PDF title screen (NASA, viewed July 6, 2010).NASA case ;LAR-15184-1.Light emitting diodesDiodes, SemiconductorLightTransmissionLight emitting diodesnasatSemiconductor diodesnasatTransmission efficiencynasatLight transmissionnasatTaperingnasatLight emitting diodes.Diodes, Semiconductor.LightTransmission.Light emitting diodes.Semiconductor diodes.Transmission efficiency.Light transmission.Tapering.Egalon Claudio Oliveira1421266Rogowski Robert S1398632United States.National Aeronautics and Space Administration.GPOGPOBOOK9910697261403321Increased efficiency LED3542088UNINA