05153nam 2200685Ia 450 991045797060332120200520144314.01-281-18650-397866111865000-08-052819-8(CKB)1000000000384305(EBL)331913(OCoLC)476132220(SSID)ssj0000112459(PQKBManifestationID)11129190(PQKBTitleCode)TC0000112459(PQKBWorkID)10086928(PQKB)11664529(MiAaPQ)EBC331913(PPN)170265250(Au-PeEL)EBL331913(CaPaEBR)ebr10216739(CaONFJC)MIL118650(EXLCZ)99100000000038430519950908d1996 uy 0engur|n|---|||||txtccrBiotechnology[electronic resource] a laboratory course /Jeffrey M. Becker, Guy A. Caldwell, Eve Ann Zachgo2nd ed.San Diego Academic Pressc19961 online resource (283 p.)Description based upon print version of record.0-12-084562-8 Includes bibliographical references and index.Front Cover; Biotechnology: A Laboratory Course; Copyright Page; Contents; Preface to the Second Edition; Preface to the First Edition; Acknowledgments; Suggested Schedule for Exercises; Introductory Notes: Record Keeping and Safety Rules; Format of Student Laboratory Records; The Ten Commandments of Record Keeping; Safety Rules in the Laboratory; Exercise 1. Aseptic Technique and Establishing Pure Cultures: The Streak Plate and Culture Transfer; Exercise 2. Preparation of Culture Media; Exercise 3. The Growth Curve; Exercise 4. Isolation of Plasmid DNA from Escherichia coli: The Mini-PrepExercise 5. Purification, Concentration, and Quantitation of DNAExercise 6. Large-Scale Isolation of Plasmid DNA by Column Chromatography; Exercise 7. Amplification of a lacZ Gene Fragment by the Polymerase Chain Reaction; Exercise 8. Restriction Digestion and Agarose Gel Electrophoresis; Exercise 9. Southern Transfer; Exercise 10. Preparation, Purification, and Hybridization of Probe; Exercise 11. Transformation of Saccharomyces cerevisiae; Exercise 12. Isolation of Plasmid from Yeast and Escherichia coil Transformation; Exercise 13. Protein AssaysExercise 14. Qualitative Assay for b-Galactosidase in Yeast ColoniesExercise 15. Determination of b-Galactosidase in Permeabilized Yeast Cells; Exercise 16. Assay of b-Galactosidase in Cell Extracts; Exercise 17. b-Galactosidase Purification; Exercise 18. Western Blot: Probe of Protein Blot with Antibody to b-Galactosidase; Appendix 1. Alternative Protocols and Experiments; Exercise 1A Isolation and Characterization of Auxotrophic Yeast Mutants; Exercise 2A Measurement of pH; Exercise 3A Use of the Spectrophotometer; Exercise 6A Isolation of Plasmid DNA: The Maxi-PrepExercise 10A Colony HybridizationAppendix 2. Buffer Solutions; Appendix 3. Preparation of Buffers and Solutions; Appendix 4. Properties of Some Common Concentrated Acids and Bases; Appendix 5. Use of Micropipettors; Appendix 6. Safe Handling of Microorganisms; Appendix 7. List of Cultures; Appendix 8. Storage of Cultures and DNA; Appendix 9. Sterilization Methods; Appendix 10. Preparation of Stock Solutions for Culture Media; Appendix 11. Growth in Liquid Medium; Appendix 12. Determination of Viable Cells; Appendix 13. Determination of Cell Mass; Appendix 14. Determination of Cell NumberAppendix 15. Nomenclature of StrainsAppendix 16. Glassware and Plasticware; Appendix 17. Preparation of Tris and EDTA; Appendix 18. Basic Rules for Handling Enzymes; Appendix 19. Effects of Common Contaminants on Protein Assays; Appendix 20. Manufacturers' and Distributors' Addresses; Appendix 21. Surfing the Bionet: World Wide Web Addresses; Glossary; IndexThe objectives of this Second Edition of Biotechnology: A Laboratory Course remain unchanged: to create a text that consists of a series of laboratory exercises that integrate molecular biology with protein biochemistry techniques while providing a continuum of experiments. The course begins with basic techniques and culminates in the utilization of previously acquired technical experience and experimental material. Two organisms, Sacchaomyces cerevisiae and Escherichia coli, a single plasmid, and a single enzyme are the experimental material, yet the procedures andBiochemical engineeringBiotechnologyLaboratory manualsElectronic books.Biochemical engineering.Biotechnology660.6660/.6/078 20Becker Jeffrey M621784Caldwell Guy A952474Zachgo Eve Ann952475MiAaPQMiAaPQMiAaPQBOOK9910457970603321Biotechnology2153260UNINA