05015nam 2200565 a 450 991045546830332120200520144314.01-283-66474-70-19-159161-00-585-48392-2(CKB)111087026789928(EBL)1043143(OCoLC)815671912(MiAaPQ)EBC1043143(Au-PeEL)EBL1043143(CaPaEBR)ebr10612575(EXLCZ)9911108702678992819970311d1997 uy 0engur|n|---|||||Subcellular fractionation[electronic resource] a practical approach /edited by J.M. Graham and D. RickwoodOxford ;New York IRL Press at Oxford University Pressc19971 online resource (360 p.)The practical approach seriesDescription based upon print version of record.0-19-963494-7 Includes bibliographical references and index.Cover; Contents; List of contributors; Abbreviations; 1. Homogenization of tissues and cells; 1. Introduction; 2. Aims of the homogenization procedure; 3. Influence of sample type; 4. Homogenization media; 5. Methods of homogenization; Type 1 homogenizers; Type 2 homogenizers; 6. Homogenization of tissues and cells; Mammalian liver; Brain; Muscle; Mammalian tissue culture cells; Plant organelles; Yeast; Other fungi and algae; Trypanosomes; Bacteria; References; 2. Isolation of subcellular fractions; 1. Introduction; 2. Composition of a tissue homogenate; 3. Properties of cell organellesFactors affecting organelle density and size4. Centrifugal methods for the separation of organelles; Separation by size; Separation by density; Density perturbation; 5. Non-centrifugal procedures; Immunoisolation; Separation by electrophoresis; 6. Identification of separated material; Marker enzymes; Introduced markers for endocytic and exocytic pathways; Characteristic non-enzymatic proteins; 7. Assessment of the purity of fractions; Purity and purification; Problems from cell heterogeneity within tissues; Problems arising from organelle fragmentationMissorting in the exocytic and endocytic pathways8. Fractionation problems; No separation; Aggregation following resuspension of a fraction; Poor recovery of markers; Damage to cell structures; 9. A systematic approach to cell fractionation; Preliminary studies; Determination of the properties of components of the homogenate; Method development; Simplification of the separation; References; 3. Isolation and characterization of nuclei and nuclear subfractions; 1. Introduction; 2. Methods of preparing purified nuclei; Types of cells and tissue samples; Homogenization mediaHomogenization methodsCentrifugation conditions; Assays of nuclear purity; 3. Methods for purifying metaphase chromosomes; 4. Isolation of nuclear subfractions; Preparation of nucleoli; Preparation of nuclear membranes; Isolation of nuclear matrix; Preparation of nucleoids; 5. Isolation of nucleoprotein complexes; Isolation of polynucleosomes of chromatin; Ribonucleoproteins; 6. Isolation of nuclear macromolecules; Isolation of nuclear proteins; Isolation of nuclear RNA; Isolation of DNA; 7. Functional assays of nuclei; Analysis of DNA-binding proteins; Transcription assays; References4. Subcellular fractionation of mitochondria1. Introduction; 2. Purification of mitochondria from various eukaryotic sources; Introduction; Protocols for purification of mitochondria from several eukaryotic sources; Further purification of mitochondrial fractions; 3. Determination of mitochondrial purity; Introduction; Use of the oxygen electrode to determine mitochondrial integrity; Determination of the integrity of the mitochondrial outer membrane; Glucose hexokinase trap method for estimation of P:O ratios; 4. Subfractionation of mitochondriaPreparation of submitochondrial particles by sonicationMany investigations into the structure and function of cells and tissues require the isolation of a particular membrane or subcellular component (organelle). This book covers all the necessary aspects, from breaking up the cells (homogenization), via a variety of separation techniques (the isolation and fractionation chapters), to characterization of the separated organelles.Practical approach series.Subcellular fractionationHandbooks, manuals, etcSubcellular fractionationLaboratory manualsElectronic books.Subcellular fractionationSubcellular fractionation571.6/5Graham J. M(John M.),1943-94317Rickwood D(David)92661MiAaPQMiAaPQMiAaPQBOOK9910455468303321Subcellular fractionation2238356UNINA05659nam 2200757 450 991079463530332120200520144314.03-11-040320-X10.1515/9783110403169(CKB)4210000000000159(EBL)1787185(SSID)ssj0001516814(PQKBManifestationID)12649770(PQKBTitleCode)TC0001516814(PQKBWorkID)11494864(PQKB)11256567(DE-B1597)444152(OCoLC)907305523(DE-B1597)9783110403169(MiAaPQ)EBC1787185(Au-PeEL)EBL1787185(CaPaEBR)ebr11049493(CaONFJC)MIL807605(OCoLC)908080002(EXLCZ)99421000000000015920150501h20152015 uy| 0engur|nu---|u||utxtccrColonialism and missionary linguistics /edited by Klaus Zimmermann and Birte Kellermeier-RehbeinBerlin :De Gruyter,[2015]©20151 online resource (276 p.)Koloniale und Postkoloniale Linguistik / Colonial and Postcolonial Linguistics ;volume 5Description based upon print version of record.3-11-036048-9 3-11-040316-1 Includes bibliographical references and index.Front matter --Contents --Preface --References --From missionary linguistics to colonial linguistics --Missionary descriptions in a colonial context --Case in selected grammars of Swahili --The first missionary linguistics in Fernando Po --Imagined communities, invented tribe? --Pre-colonial language policy of the Rhenish Mission Society perceived as the type of Gustav Warneck’s mission doctrine? --Reducing languages to writing --Transculturation, assimilation, and appropriation in the missionary representation of Nahuatl --Connections between the scientific discourse and the frontier missions in the surroundings of the Viceroyalty of New Granada --Examples of transcultural processes in two colonial linguistic documents on Jebero (Peru) --Index of Persons (including authors) --Index of Languages --Index of SubjectsA lot of what we know about “exotic languages” is owed to the linguistic activities of missionaries. They had the languages put into writing, described their grammar and lexicon, and worked towards a standardization, which often came with Eurocentric manipulation. Colonial missionary work as intellectual (religious) conquest formed part of the Europeans' political colonial rule, although it sometimes went against the specific objectives of the official administration. In most cases, it did not help to stop (or even reinforced) the displacement and discrimination of those languages, despite oftentimes providing their very first (sometimes remarkable, sometimes incorrect) descriptions. This volume presents exemplary studies on Catholic and Protestant missionary linguistics, in the framework of the respective colonial situation and policies under Spanish, German, or British rule. The contributions cover colonial contexts in Latin America, Africa, and Asia across the centuries. They demonstrate how missionaries dealing with linguistic analyses and descriptions cooperated with colonial institutions and how their linguistic knowledge contributed to European domination.Ein Großteil des Wissens über „exotische“ Sprachen verdanken wir den linguistischen Aktivitäten der Missionare. Ihr Beitrag belief sich v.a. auf die Verschriftlichung, die Beschreibung der Grammatik und Lexik sowie auf die Standardisierung, was in manchen Fällen mit einer eurozentristischen Manipulation der beschriebenen Sprachen einherging. Insgesamt war die koloniale Missionierung als geistige (religiöse) Eroberung Teil der politischen Kolonialherrschaft, auch wenn sie manchmal im Widerstreit zur Kolonialverwaltung stand. Die Verdrängung und Diskriminierung der Sprachen hat sie jedenfalls, trotz der meistens ersten, z.T. bewundernswerten, z.T. fehlerhaften Beschreibungen selten aufhalten können, bisweilen sogar daran mitgewirkt. Der Band bietet exemplarische Studien über katholische und protestantische Missionarslinguistik unter den Bedingungen der jeweils geltenden Kolonialpolitik unter spanischer, deutscher und britischer Verwaltung in Hispanoamerika, Afrika und Asien in verschiedenen Jahrhunderten. Sie zeigen, wie sprachbeschreibend tätige Missionare mit den Kolonialverwaltungen kooperierten und mit ihrem Wissen zur Herrschaft beitrugen.Koloniale und postkoloniale Linguistik ;Band 5.MissionsLinguistic workCommunication, InternationalColoniesHistoryLanguage and languagesStudy and teachingHistoryColonial Linguistics.Historical Grammar.Missionary Linguistics.MissionsLinguistic work.Communication, International.ColoniesHistory.Language and languagesStudy and teachingHistory.400Zimmermann KlausKellermeier-Rehbein BirteInternational Conference on Missionary Linguistics(7th :2012 :Bremen, Germany)MiAaPQMiAaPQMiAaPQBOOK9910794635303321Colonialism and missionary linguistics3825042UNINA