03196nam 2200841z- 450 991040408430332120231214133245.03-03928-255-7(CKB)4100000011302295(oapen)https://directory.doabooks.org/handle/20.500.12854/50093(EXLCZ)99410000001130229520202102d2020 |y 0engurmn|---annantxtrdacontentcrdamediacrrdacarrierIn-Cell NMR Spectroscopy: Biomolecular Structure and FunctionMDPI - Multidisciplinary Digital Publishing Institute20201 electronic resource (152 p.)3-03928-254-9 This Special Issue examines state-of-the-art in-cell NMR spectroscopy as it relates to biological systems of increasing complexity. The compendia of research and recent innovations from prominent laboratories in the field of solid state and solution in-cell NMR spectroscopy, metabolomics and technology development are presented. The work establishes in-cell NMR spectroscopy as the premier method for determining the structures and interaction capabilities of biological molecules at high resolution within the delicately intricate interior of living cells, and the means of utilizing cells as living laboratories to directly assess the effects of exogenous and endogenous stimuli on cell physiology.]In-Cell NMR Spectroscopyprotein NMRtime-resolved NMRRibosomestructural calculation 4crystalline and amorphous starchin-cell NMRprotein dynamicsDNPprotein modificationTauspectrum reconstruction 3mRNAThioredoxinprotein structureprotein interactionsdrug discoveryprotein structure determination 1reviewenzyme activityMARK2 phosphorylationpost-translational modificationsDihydrofolate reductasemammalian cellstarget engagementnon-uniform sampling 2paramagnetic effectsprotein structure-functioncross-correlated relaxationstructure functionrRNA2D INADEQUATElipid membraneThymidylate synthasewhole cell NMRenzyme kineticsmagic-angle spinninglive cellsolid-state NMRAdenylate kinaseDNAin-situ NMRantimicrobial peptideNMR spectroscopyintrinsically disordered proteinsBurz David Sauth1278676Shekhtman AlexanderauthBOOK9910404084303321In-Cell NMR Spectroscopy: Biomolecular Structure and Function3013715UNINA