03128nam 2200757z- 450 9910372785403321202102113-03928-023-6(CKB)4100000010163772(oapen)https://directory.doabooks.org/handle/20.500.12854/42862(oapen)doab42862(EXLCZ)99410000001016377220202102d2020 |y 0engurmn|---annantxtrdacontentcrdamediacrrdacarrierCell-Free Synthetic BiologyMDPI - Multidisciplinary Digital Publishing Institute20201 online resource (152 p.)3-03928-022-8 Cell-free synthetic biology is in the spotlight as a powerful and rapid approach to characterize and engineer natural biological systems. The open nature of cell-free platforms brings an unprecedented level of control and freedom for design compared to in vivo systems. This versatile engineering toolkit is used for debugging biological networks, constructing artificial cells, screening protein library, prototyping genetic circuits, developing new drugs, producing metabolites, and synthesizing complex proteins including therapeutic proteins, toxic proteins, and novel proteins containing non-standard (unnatural) amino acids. The book consists of a series of reviews, protocols, benchmarks, and research articles describing the current development and applications of cell-free synthetic biology in diverse areas.History of engineering and technologybicsscantimicrobialsartificial cellbioconversion platformcell-free metabolic engineeringcell-free metabolic engineering (CFME)cell-free protein expression (CFPE)cell-free protein synthesiscell-free protein synthesis (CFPS)cell-free synthetic biologycell-free transcription-translationCFPSchaperonescolicinsDNA origamidrug developmentE. coli crude extract preparationenzymeseukaryotic lysatesgenomically engineered E. coligrowth factorsin vitro protein synthesisin vitro transcription-translation (TX-TL)in vitro translationischemic strokemathematical modelmicrosomesn/aprotein productionrapid prototypingriboregulatorsonicationsynthetic biologytissue plasminogen activatortPaunnatural amino acidunnatural proteinHistory of engineering and technologyHong Seok Hoonauth1291812BOOK9910372785403321Cell-Free Synthetic Biology3021952UNINA