02975nam 2200709z- 450 991037278540332120231214133532.03-03928-023-6(CKB)4100000010163772(oapen)https://directory.doabooks.org/handle/20.500.12854/42862(EXLCZ)99410000001016377220202102d2020 |y 0engurmn|---annantxtrdacontentcrdamediacrrdacarrierCell-Free Synthetic BiologyMDPI - Multidisciplinary Digital Publishing Institute20201 electronic resource (152 p.)3-03928-022-8 Cell-free synthetic biology is in the spotlight as a powerful and rapid approach to characterize and engineer natural biological systems. The open nature of cell-free platforms brings an unprecedented level of control and freedom for design compared to in vivo systems. This versatile engineering toolkit is used for debugging biological networks, constructing artificial cells, screening protein library, prototyping genetic circuits, developing new drugs, producing metabolites, and synthesizing complex proteins including therapeutic proteins, toxic proteins, and novel proteins containing non-standard (unnatural) amino acids. The book consists of a series of reviews, protocols, benchmarks, and research articles describing the current development and applications of cell-free synthetic biology in diverse areas.genomically engineered E. coliin vitro transcription-translation (TX-TL)tPatissue plasminogen activatorin vitro translationenzymesDNA origamiantimicrobialscell-free metabolic engineering (CFME)cell-free transcription-translationin vitro protein synthesiscell-free metabolic engineeringE. coli crude extract preparationchaperonesischemic strokemicrosomessonicationcell-free protein synthesisgrowth factorsartificial cellunnatural proteincell-free synthetic biologyrapid prototypingdrug developmentunnatural amino acideukaryotic lysatescell-free protein expression (CFPE)riboregulatorsynthetic biologymathematical modelCFPScell-free protein synthesis (CFPS)bioconversion platformcolicinsprotein productionHong Seok Hoonauth1291812BOOK9910372785403321Cell-Free Synthetic Biology3021952UNINA