03451nam 2200769z- 450 9910367745503321202102113-03921-701-1(CKB)4100000010106262(oapen)https://directory.doabooks.org/handle/20.500.12854/40355(oapen)doab40355(EXLCZ)99410000001010626220202102d2019 |y 0engurmn|---annantxtrdacontentcrdamediacrrdacarrierAdvances in Single Molecule, Real-Time (SMRT) SequencingMDPI - Multidisciplinary Digital Publishing Institute20191 online resource (128 p.)3-03921-700-3 PacBio's single-molecule real-time (SMRT) sequencing technology offers important advantages over the short-read DNA sequencing technologies that currently dominate the market. This includes exceptionally long read lengths (20 kb or more), unparalleled consensus accuracy, and the ability to sequence native, non-amplified DNA molecules. From fungi to insects to humans, long reads are now used to create highly accurate reference genomes by de novo assembly of genomic DNA and to obtain a comprehensive view of transcriptomes through the sequencing of full-length cDNAs. Besides reducing biases, sequencing native DNA also permits the direct measurement of DNA base modifications. Therefore, SMRT sequencing has become an attractive technology in many fields, such as agriculture, basic science, and medical research. The boundaries of SMRT sequencing are continuously being pushed by developments in bioinformatics and sample preparation. This book contains a collection of articles showcasing the latest developments and the breadth of applications enabled by SMRT sequencing technology.Advances in Single Molecule, Real-TimeBiology, life sciencesbicsscallele-specific analysisbone marrow cell subpopulationsCladobotryum protrusumcobweb diseasecytochrome P450 enzyme (CYP)de novo assemblyde novo genome assemblyDNA methylationfull length RNAseqgene expressionGloeostereum incarnatumGRCh38human reference genomehuman whole-genome sequencinginsect genomelong-read SMRT sequencinglow-input DNAmosquitomRNA isoformsmycoparasiten/anext generation sequencingoptical mappingPacBioPacBio single molecule real-time sequencingpopulation sequencingprotein isoformssecondary metabolitesingle molecule real-time sequencingSMRT sequencingstatistical methodsSwedish populationterpenoidTricoplusia niwhole genome sequencingBiology, life sciencesAmeur Adamauth1290162Hestand Matthew SauthBOOK9910367745503321Advances in Single Molecule, Real-Time (SMRT) Sequencing3021370UNINA