03330nam 2200721z- 450 991036774550332120231214132950.03-03921-701-1(CKB)4100000010106262(oapen)https://directory.doabooks.org/handle/20.500.12854/40355(EXLCZ)99410000001010626220202102d2019 |y 0engurmn|---annantxtrdacontentcrdamediacrrdacarrierAdvances in Single Molecule, Real-Time (SMRT) SequencingMDPI - Multidisciplinary Digital Publishing Institute20191 electronic resource (128 p.)3-03921-700-3 PacBio’s single-molecule real-time (SMRT) sequencing technology offers important advantages over the short-read DNA sequencing technologies that currently dominate the market. This includes exceptionally long read lengths (20 kb or more), unparalleled consensus accuracy, and the ability to sequence native, non-amplified DNA molecules. From fungi to insects to humans, long reads are now used to create highly accurate reference genomes by de novo assembly of genomic DNA and to obtain a comprehensive view of transcriptomes through the sequencing of full-length cDNAs. Besides reducing biases, sequencing native DNA also permits the direct measurement of DNA base modifications. Therefore, SMRT sequencing has become an attractive technology in many fields, such as agriculture, basic science, and medical research. The boundaries of SMRT sequencing are continuously being pushed by developments in bioinformatics and sample preparation. This book contains a collection of articles showcasing the latest developments and the breadth of applications enabled by SMRT sequencing technology.Advances in Single Molecule, Real-TimeCladobotryum protrusumallele-specific analysislow-input DNAfull length RNAseqde novo genome assemblyde novo assemblyhuman reference genomeTricoplusia niPacBio single molecule real-time sequencingsecondary metaboliteprotein isoformsbone marrow cell subpopulationsDNA methylationmycoparasitehuman whole-genome sequencingGRCh38SMRT sequencingcytochrome P450 enzyme (CYP)mRNA isoformsnext generation sequencingcobweb diseaseSwedish populationmosquitolong-read SMRT sequencingwhole genome sequencingterpenoidinsect genomeoptical mappingGloeostereum incarnatumpopulation sequencingstatistical methodsgene expressionsingle molecule real-time sequencingPacBioAmeur Adamauth1290162Hestand Matthew SauthBOOK9910367745503321Advances in Single Molecule, Real-Time (SMRT) Sequencing3021370UNINA